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Interaction of phytohormone-producing rhizobia with sugarcane mini-setts and their effect on plant development

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Abstract

Aims

Rhizobia are common members of plant microbiomes. This study aimed to evaluate if rhizobia can colonize sugarcane mini-setts and if and how they affect sprouting and morphological traits of plant development.

Methods

Bradyrhizobium sacchari strain BR 10280T and Rhizobium sp. strain BR 10268 were inoculated on sugarcane mini-setts and sprouting and plant development were evaluated. Bacterial production of hormones and their effect on plant growth were studied using HPLC and GC-MS / SIM and sugarcane sprouting assays. Colonization was investigated by colony counting and CLSM. Sequence analysis of recA, gyrB and rpoB genes was applied to refine the phylogenetic classification of strain BR 10268.

Results

BR 10268 had a positive effect on sugarcane mini-sett sprouting and shoot and root growth at 30 and 75 days after transplantation (DAT) and colonized sprouting mini-sett tissues. Few significant effects were observed for B. sacchari BR 10280T. Cell-free BR 10268 supernatant was found to contain gibberellins (GAs) and low concentrations of indole-3-acetic acid (IAA) and trans-zeatin; treatment of mini-setts with GA3 (0.15 or 1.5 μg mL−1) affected sprouting in a similar manner as BR 10268 inoculation. BR 10268 was found to be phylogenetically close to R. freirei.

Conclusions

Rhizobium sp. BR 10268 positively affects sprouting and growth of sugarcane, probably via production of plant hormonal substances, notably gibberellins, thus naturally occurring rhizobia may have potential as sugarcane inoculants.

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Abbreviations

CFU:

Colony forming units

TY:

Tryptone yeast extract

YMA:

Yeast extract mannitol agar

DAI:

Days after inoculation

DAT:

Days after transplanting

HPLC:

High performance liquid chromatography

GC-MS / SIM :

Gas chromatography-mass spectrometry in selective ionic monitoring mode

O.D.:

Optical density

GAx/GAs:

Gibberellin x / gibberellins

CLSM:

Confocal laser scanning microscopy

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Acknowledgements

This study was supported by Embrapa, National Council for Scientific and Technological Development (CNPq, grants 420746/2016-1 and 308898/2017-6) and Newton Fund grant BB/N013476/1 “Understanding and Exploiting Biological Nitrogen Fixation for Improvement of Brazilian Agriculture”, co-funded by the Biotechnology and Biological Sciences Research Council (BBSRC) and the Brazilian National Council for State Funding Agencies (CONFAP). The first author received a fellowship from Coordination of Superior Level Staff Improvement (CAPES). We thank Dr. Philip Poole for providing the plasmid pLMB426.

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  1. Natália S. Ferreira and Gustavo F. Matos contributed equally to this work.

Authors and Affiliations

  1. Curso de Pós-graduação em Fitossanidade e Biotecnologia Aplicada, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil

    Natália S. Ferreira

  2. Curso de Pós-graduação em Fitotecnia, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil

    Gustavo F. Matos

  3. Departamento de Biologia, Centro de Ciências Biológicas e da Saúde, Universidade Estadual da Paraíba, Campina Grande, PB, Brazil

    Carlos H. S. G. Meneses

  4. Embrapa Agrobiologia, Seropédica, RJ, Brazil

    Veronica M. Reis, Janaina R. C. Rouws, Stefan Schwab, José I. Baldani & Luc F. M. Rouws

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Ferreira, N.S., Matos, G.F., Meneses, C.H.S.G. et al. Interaction of phytohormone-producing rhizobia with sugarcane mini-setts and their effect on plant development. Plant Soil 451, 221–238 (2020). https://doi.org/10.1007/s11104-019-04388-0

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