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Inheritance of faba bean resistance to Broomrape, genetic diversity and QTL mapping analysis

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Abstract

Six faba bean parents and their F1 and F2 generations were used in this investigation to study the genetic system controlling resistance of faba bean (Vicia faba L.) to broomrape (Orobanche crenata). Most of the F1 hybrids were tolerant to broomrape. In the F2 generation, the population P5 × P6 (Assiut 125 × Romy 12) gave the highest value of relative yield and tolerance index. Heterosis and inbreeding depression were only positive in number of tillers/plant and seed yield/plant characters. The results indicated that the additive effect was more important than the dominance one (D > H1) only for No. of pods/plant in the F1 generation. Moreover, the narrow-sense heritability was low for most of the studied traits. Three molecular marker systems, namely RAPD, ISSR and SRAP were used for identification and estimation of the genetic diversity among the six faba bean genotypes. The three molecular markers generated DNA unique bands for all genotypes. Only, eight DNA fragments were related to Orobanche tolerance. Clearly and reproducible polymorphic markers were subjected to QTL analysis. The linkage analysis showed that, out of 34 marker loci segregated in the F2 population, 29 (85.29%) were mapped on three linkage groups. QTL analysis using SIM method performed for the 29 markers assigned to LG-1, LG-2 and LG-3 with the eight traits, number of tillers/plant, plant height, number of pods/plant, seed yield/plant, number of broomrape spikes per plant, height of broomrape spikes, relative yield and tolerance index, showing 12 putative QTLs for all traits except number of tillers/plant. From this study, it is clear that P5 × P6 (Assiut 125 × Romy12) population could be considered promising for selection for resistance to broomrape infestation.

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Acknowledgement

The authors would like to deeply appreciate the support provided by the Faculty of Agriculture, Assiut University, Egypt.

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  1. Department of Genetics, The Faculty of Agriculture, Assiut University, Assiut, 71526, Egypt

    Bahaa E. S. Abd El-Fatah

  2. Department of Vegetables, The Faculty of Agriculture, Assiut University, Assiut, 71526, Egypt

    Dalia M. T. Nassef

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Abd El-Fatah, B.E.S., Nassef, D.M.T. Inheritance of faba bean resistance to Broomrape, genetic diversity and QTL mapping analysis. Mol Biol Rep 47, 11–32 (2020). https://doi.org/10.1007/s11033-019-05101-1

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