Abstract
An effective evaluation model was established to digitize the quality of cocoa butter equivalents (CBEs) based on determinations of total and sn-2 fatty acid compositions and triacylglycerol (TAG) profiles and the “deducting score” principle. Similarity scores for selected fats and oils calculated from the model revealed differences between them and parallel cocoa butter compositions. For CBE1 and CBE2, total similarity scores were 90.6 and 90.0, whereas those of mango (76.3), dhupa (84.1), sal fat (84.7), kokum (78.3), palm mid fractions (PMF, 77.9), shea butter (64.0), illipe butter (89.7) and Pentadesma butyracea butter (67.2), respectively. Similarity scores were found to agree with physical properties, including polymorphism, crystal morphology, crystallization or melting behaviors, and solid fat content. The present study provides an accurate means of assessing CBE quality and hopefully will contribute to the development of commercial CBEs.
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References
Adhikari P, Shin JA, Lee JH, Hu JN, Hwang K, Lee KT. Enzymatic production of trans-free hard fat stock from fractionated rice bran oil, fully hydrogenated soybean oil, and conjugated linoleic acid. J. Food Sci. 74: E87–E96 (2009)
Barrett CB, Dallas MS, Padley FB. The quantitative analysis of triglyceride mixtures by thin layer chromatography on silica impregnated with silver nitrate. J. Am. Oil Chem. Soc. 40: 580–584 (1963)
Bootello MA, Hartel RW, Garcés R, Martínez-Force E, Salas JJ. Evaluation of high oleic-high stearic sunflower hard stearins for cocoa butter equivalent formulation. Food Chem. 134: 1409–1417 (2012)
Bracco U. Effect of triglyceride structure on fat absorption. Am. J. Clin. Nutr. 60: 1002S–1009S (1994)
Buchgraber M, Ulberth F, Anklam E. Cluster analysis for the systematic grouping of genuine cocoa butter and cocoa butter equivalent samples based on triglyceride patterns. J. Agric. Food Chem. 52: 3855–3860 (2004)
Coleman MH. Further studies on the pancreatic hydrolysis of some natural fats. J. Am. Oil Chem. Soc. 38: 685–688 (1961)
Compton DL, Laszlo JA, Eller FJ, Taylor SL. Purification of 1, 2-diacylglycerols from vegetable oils: comparison of molecular distillation and liquid CO2 extraction. Ind. Crop. Prod. 28: 113–121 (2008)
Gan LJ, Yang D, Shin JA, Kim SJ, Hong ST, Lee JH, Sung CK, Lee KT. Oxidative comparison of emulsion systems from fish oil-based structured lipid versus physically blended lipid with purple-fleshed sweet potato (Ipomoea batatas L.) extracts. J. Agric. Food Chem. 60: 467–475 (2011)
Garti N, Widlak NR. Cocoa Butter and Related Compounds. 1st ed. AOCS Press, Champaign, IL (2012)
Gunstone FD, Harwood JL, Dijkstra AJ. The Lipid Handbook. 3rd ed. CRC Press, Boca Raton, FL (2007)
Hatzakis E, Agiomyrgianaki A, Kostidis S, Dais P. High-resolution NMR spectroscopy: an alternative fast tool for qualitative and quantitative analysis of diacylglycerol (DAG) oil. J. Am. Oil Chem. Soc. 88: 1695–1708 (2011)
Jia CH, Shin JA, Lee KT. Effects of caffeic acid phenethyl ester and 4-vinylcatechol on the stabilities of oil-in-water emulsions of stripped soybean oil. J. Agric. Food Chem. 63: 10280–10286 (2015)
Larsson K. Classification of glyceride crystal forms. Acta Chem. Scand. 20: 2255–2260 (1966)
Lee JH, Lee KT, Akoh CC, Chung SK, Kim MR. Antioxidant evaluation and oxidative stability of structured lipids from extravirgin olive oil and conjugated linoleic. Acid. J. Agric. Food Chem. 54: 5416–5421 (2006)
Lipp M, Simoneau C, Ulberth F, Anklam E, Crews C, Brereton P, De Greyt W, Schwack W, Wiedmaier C. Composition of genuine cocoa butter and cocoa butter equivalents. J. Food Compos. Anal. 14: 399–408 (2001)
Miura S, Konishi H. Crystallization behavior of 1, 3-dipalmitoyl-2-oleoyl-glycerol and 1-palmitoyl-2, 3-dioleoyl-glycerol. Eur. J. Lipid Sci. Technol. 103: 804–809 (2001).
Petersson B, Anjou K, Sandström L. Pulsed NMR method for solid fat content determination in tempering fats, Part I: Cocoa butters and equivalents. Eur. J. Lipid Sci. Technol. 87: 225–230 (1985)
Qin XL, Zhong JF, Wang YH, Yang B, Lan DM, Wang FH. 1, 3-Dioleoyl-2-palmitoylglycerol-rich human milk fat substitutes: Production, purification, characterization and modeling of the formulation. Eur. J. Lipid Sci. Technol. 116: 282–290 (2014)
Shin JA, Akoh CC, Lee KT. Production and physicochemical properties of functional-butterfat through enzymatic interesterification in a continuous reactor. J. Agric. Food Chem. 57: 888–900 (2009)
Sonwai S, Kaphueakngam P, Flood A. Blending of mango kernel fat and palm oil mid-fraction to obtain cocoa butter equivalent. J. Food Sci. Technol. 51: 2357–2369 (2014)
Sridhar R, Lakshminarayana G, Kaimal TNB. Modification of selected Indian vegetable fats into cocoa butter substitutes by lipase-catalyzed ester interchange. J. Am. Oil Chem. Soc. 68: 726–730 (1991)
Sun XY, Bi YL, Yang GL. Composition and properties analysis of cocoa butter replacer, cocoa butter equivalent and cocoa butter. China Oils Fats 32: 38–42 (2007)
Swern D. Bailey’s industrial oil and fat products. 2nd ed. Wiley, Indianapolis, IN (1982)
Talbot G, Slager H. Cocoa butter equivalents and improvers-Their use in chocolate and chocolate-coated confectionery. Agro Food Ind. Hi-Tech. 19: 28–29 (2008)
Tautorus CL, McCurdy AR. Effect of randomization on oxidative stability of vegetable oils at two different temperatures. J. Am. Oil Chem. Soc. 67: 525–530 (1990)
Tchobo FP, Piombo G, Pina M, Soumanou MM, Villeneuve P, Sohounhloue DCK. Enzymatic synthesis of cocoa butter equivalent through transesterification of Pentadesma Butyracea butter. J. Food Lipids 16: 605–617 (2009)
Tietz RA, Hartel RW. Effects of minor lipids on crystallization of milk fat-cocoa butter blends and bloom formation in chocolate. J. Am. Oil Chem. Soc. 77: 763–771 (2000)
Timms RE, Stewart IM. Cocoa butter, a unique vegetable fat. Lipid Technol. 5: 101–107 (1999)
Van Malssen KF, Peschar R, Schenk H. Real-time X-ray powder diffraction investigations on cocoa butter. II. The relationship between melting behavior and composition of β-cocoa butter. J. Am. Oil Chem. Soc. 73: 1217–1223 (1996)
Wang YH, Mai QY, Qin XL, Yang B, Wang ZL, Chen HT. Establishment of an evaluation model for human milk fat substitutes. J. Agric. Food Chem. 58: 642–649 (2009).
Zou XQ, Huang JH, Jin QZ, Guo Z, Liu YF, Cheong LZ, Xu XB, Wang XG. Model for human milk fat substitute evaluation based on triacylglycerol composition profile. J. Agric. Food Chem. 61: 167–175 (2012)
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Jia, CH., Shin, JA. & Lee, KT. Evaluation model for cocoa butter equivalents based on fatty acid compositions and triacylglycerol patterns. Food Sci Biotechnol 28, 1649–1658 (2019). https://doi.org/10.1007/s10068-019-00630-8
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DOI: https://doi.org/10.1007/s10068-019-00630-8