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Disturbed Gastrointestinal Contractility in a Polycystic Ovary Syndrome Rat Model

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A Correction to this article was published on 20 April 2020

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Abstract

Background

Polycystic ovary syndrome (PCOS), a common hormonal disorder in women, affects 4–18% of women of reproductive age worldwide. A higher prevalence of irritable bowel syndrome was found in women with PCOS. However, the effects and mechanism of PCOS on stomach and colon contractility remain unclear.

Aims

This study aims to evaluate the correlation between PCOS and gastrointestinal disorder.

Methods

Four-week-old female rats were subcutaneously implanted with pellets containing 7.5 mg of dihydrotestosterone for 13 weeks to create PCOS rat models. After vaginal smears, the estrus cycle stage was evaluated. Oral glucose tolerance test was performed after 90 days of treatment. All animals were killed at 17 weeks. The rats were fasted overnight and then anesthetized before decapitation, and the stomach fundus and colon were surgically removed and cultured in oxygenated Krebs solution. Acetylcholine and carbachol were used to evaluate the cholinergic system on contractility.

Results

The basal and stomach fundus responded with a reduced frequency and contractility in response to acetylcholine in the PCOS group. Moreover, no difference was found in the spontaneous stomach contractility induced by carbachol in both groups. Lower maximal colon muscle contractility was also found in response to acetylcholine stimulation in PCOS rats. Furthermore, lower maximal muscle contractility was found in response to extracellular calcium levels. MLC20 phosphorylation was also reduced in the gastrointestinal tissue in PCOS rats.

Conclusions

PCOS induces gastroparesis and reduces gastrointestinal muscle contractility. This effect is, at least partly, through reducing the responsiveness of acetylcholine and MLC20 phosphorylation.

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Change history

  • 20 April 2020

    The original version of the article unfortunately contained an error in the legend of Figure��5B. Corrected version of Figure��5 is given below.

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Funding

This study was funded by a grant (107-2320-B-254 -001) from the National Science Council, Taipei, Taiwan, ROC.

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Authors and Affiliations

  1. Department of Nursing, Ching Kuo Institute of Management and Health, No. 336, Fuxsin Rd., Zhongshan Dist., Keelung City, 20301, Taiwan, Republic of China

    Kai-Lee Wang

  2. Department of Physiology, School of Medicine, National Yang-Ming University, Taipei, Taiwan, Republic of China

    Kai-Lee Wang & Paulus S. Wang

  3. School of Nutrition and Health Sciences, Taipei Medical University, Taipei, 11031, Taiwan, Republic of China

    Shih-Min Hsia & Po-Han Lin

  4. Department of Medical Research and Education, Taipei Veterans General Hospital, Taipei, Taiwan, Republic of China

    Paulus S. Wang

  5. PhD Program of Aging, College of Medicine, China Medical University, Taichung, Taiwan, Republic of China

    Paulus S. Wang

  6. Department of Biotechnology, Asia University, Taichung, Taiwan, Republic of China

    Paulus S. Wang

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  1. Kai-Lee Wang
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  2. Shih-Min Hsia
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Contributions

Study concepts, study design, data acquisition, data analysis and interpretation, statistical analysis, and manuscript preparation: K.-L. Wang. Animal preparation: P.-H. Lin. Manuscript editing and review: S.-M. Hsia and P. S. Wang.

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Correspondence to Kai-Lee Wang.

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All applicable international, national, and institutional guidelines for the care and use of animals were followed.

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Wang, KL., Hsia, SM., Wang, P.S. et al. Disturbed Gastrointestinal Contractility in a Polycystic Ovary Syndrome Rat Model. Dig Dis Sci 65, 2834–2843 (2020). https://doi.org/10.1007/s10620-019-06001-x

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