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Isolation and characterization of the novel Virgibacillus-infecting bacteriophage Mimir87

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Abstract

The novel bacterial virus Mimir87, infecting the salt-tolerant bacterium Virgibacillus halotolerans, was isolated from worker honey bees. Mimir87 has an elongated head and a long non-contractile tail consistent with members of the Siphoviridae phage family. The phage genome comprises 48,016 base pairs and encodes 68 predicted proteins, to 34 of which a function could be assigned from homology analysis. The phage encodes two metabolism-related transporter proteins previously not observed in bacteriophage genomes. Mimir87 displays some relatedness to several Bacillus and Paenibacillus viruses; however, the overall sequence dissimilarity suggests Mimir87 to be a representative of a new phage genus.

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Acknowledgements

We would like to express our gratitude for the valuable feedback and advice given on the draft version of the manuscript to Professor Rob Lavigne (Katholieke Universiteit Leuven). We also sincerely thank Dr. Janis Rumnieks (Latvian Biomedical Research and Study Centre) for his critical review of the final version of the manuscript.

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Author notes

  1. Nikita Zrelovs and Elina Cernooka contributed equally.

Authors and Affiliations

  1. Latvian Biomedical Research and Study Centre, Ratsupites 1 k-1, Riga, LV-1067, Latvia

    Nikita Zrelovs, Elina Cernooka, Andris Dislers & Andris Kazaks

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  1. Nikita Zrelovs
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  2. Elina Cernooka
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  3. Andris Dislers
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  4. Andris Kazaks
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Correspondence to Andris Kazaks.

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Zrelovs, N., Cernooka, E., Dislers, A. et al. Isolation and characterization of the novel Virgibacillus-infecting bacteriophage Mimir87. Arch Virol 165, 737–741 (2020). https://doi.org/10.1007/s00705-019-04516-2

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  • DOI: https://doi.org/10.1007/s00705-019-04516-2

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