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Development and application of an indirect ELISA for the serological detection of duck Tembusu virus infection based on the NS1 protein antigen

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Abstract

Duck Tembusu virus (DTMUV) has caused significant economic losses in China since 2010. However, there is still a lack of effective methods to diagnose the disease caused by this virus, and especially to differentiate infection from vaccination. In this study, we established a novel indirect enzyme-linked immunosorbent assay (iELISA) and performed a retrospective serological survey for DTMUV in Anhui province, China. Our results show that the iELISA displayed high specificity sensitivity, and with no serological cross-reaction with other duck pathogens. These findings indicate that the newly developed iELISA could be a useful screening tool for large-scale monitoring of the epidemiology of DTMUV infection in ducks.

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Funding

This study was supported by the Key Research Project of National Science and Technology (no. 2016YFD0500805 and no. 2018YFD0500100), the Key Project of Department of Science and Technology in Anhui Province (no. 11070303024) and the Key Project of the Educational Commission of Anhui Province of China (no. KJ2012A124).

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Author notes

  1. Qi Zhou and Zhuangli Bi contributed equally to this article.

Authors and Affiliations

  1. College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, China

    Qi Zhou, Zhuangli Bi, Dongdong Yin, Xiangxue Gu, Zejun Xu, Rong Huang, Xue Xing & Guijun Wang

  2. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China

    Zhuangli Bi

  3. Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, Hefei, 230036, China

    Dongdong Yin, Kezong Qi & Guijun Wang

Authors
  1. Qi Zhou
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  2. Zhuangli Bi
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  3. Dongdong Yin
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  4. Xiangxue Gu
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Corresponding authors

Correspondence to Kezong Qi or Guijun Wang.

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All authors declare that they have no competing interests.

Ethical approval

We confirm that all animal experiments were carried out in accordance with the guidelines of the Guide for the Care and Use of Laboratory Animal of the Institutional Animal Care and Use Committee (IACUC) set by Anhui Agricultural University. All animal experiments were approved by the Animal Care and Use Committee of Anhui Agricultural University (approval number AHAU2016-31).

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Zhou, Q., Bi, Z., Yin, D. et al. Development and application of an indirect ELISA for the serological detection of duck Tembusu virus infection based on the NS1 protein antigen. Arch Virol 165, 709–714 (2020). https://doi.org/10.1007/s00705-019-04495-4

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  • DOI: https://doi.org/10.1007/s00705-019-04495-4

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