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Genetic variability of pig and human rotavirus group A isolates from Slovakia

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Abstract

The aim of this work was the genetic typing of RVA isolates originating from pigs and human patients in Slovakia. Seventy-eight rectal swabs from domestic pigs and 30 stool samples from humans were collected. The whole VP7 (G genotypes), VP6 (I genotypes) and partial VP4 (P genotypes) ORFs were amplified by RT-PCR. Genetic variability was higher amongst porcine sequences, where four G genotypes (G3, G4, G5, G11), two P genotypes (P[6], P[13]) and one I5 genotype were detected. Human RVA strains were represented by two G genotypes (G1, G3), two I genotypes (I1, I2), and one P genotype (P[8]). Genetic analysis did not show a relationship between Slovakian porcine and human RVA strains, but phylogenetic grouping of some Slovakian porcine sequences with Hungarian human sequences in both G and P genotypes was observed.

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Acknowledgments

We would like to thank Peter Nettleton, Edinburgh, for critical reading of the manuscript and correction of English grammar. This work was supported by projects APVV-15-0415 from The Slovak Research and Development Agency and Medical University Park in Košice (MediPark, Košice) ITMS: 26220220185 supported by Operational Programme Research and Development (OP VaV-2012/2.2/08-RO) (contract no. OPVaV/12/2013).

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Authors and Affiliations

  1. Department of Epizootiology and Preventive Veterinary Medicine, University of Veterinary Medicine and Pharmacy, Komenskeho 73, 041 81, Kosice, Slovakia

    S. Salamunova, A. Jackova, R. Mandelik & S. Vilcek

  2. Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy, Komenskeho 73, 041 81, Kosice, Slovakia

    T. Csank

  3. Clinic of Pigs, University of Veterinary Medicine and Pharmacy, Komenskeho 73, 041 81, Kosice, Slovakia

    J. Novotny

  4. Department of Clinical Microbiology, F.D. Roosevelt University Hospital, Nám. L. Svobodu 1, 975 17, Banská Bystrica, Slovakia

    Z. Beckova & L. Helmova

  5. St. Elizabeth University of Health and Social sciences, Palackého 1, 810 00, Bratislava, Slovakia

    Z. Beckova

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  1. S. Salamunova
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  2. A. Jackova
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Correspondence to S. Vilcek.

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Salamunova, S., Jackova, A., Csank, T. et al. Genetic variability of pig and human rotavirus group A isolates from Slovakia. Arch Virol 165, 463–470 (2020). https://doi.org/10.1007/s00705-019-04504-6

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  • DOI: https://doi.org/10.1007/s00705-019-04504-6

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