Issue 3, 2020
From the journal:

Environmental Science: Nano

The adsorption behaviour of carbon nanodots modulated by cellular membrane potential†

Shuyuan Zhang,‡a   Changqing Xiao,‡e   Hang He,a   Ziqiang Xu, ORCID logo *a   Beibei Wang,b   Xueqin Chen,a   Cao Li,a   Bingbing Jianga  and  Yi Liu*bcd  

* Corresponding authors

a Ministry-of-Education Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei Key Laboratory of Polymer Materials, School of Materials Science & Engineering, Hubei University, Wuhan 430062, China
E-mail: ziqiang.xu@hubu.edu.cn

b Key Laboratory of Analytical Chemistry for Biology and Medicine (MOE), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P. R. China
E-mail: yiliuchem@whu.edu.cn

c Guangxi Key Laboratory of Natural Polymer Chemistry, College of Chemistry and Material Sciences, Nanning Normal University, Nanning 530001, P. R. China

d Key Laboratory of Coal Conversion and New Carbon Materials of Hubei Province, College of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan 430081, P. R. China

e School of Chemistry and Life Sciences, Hubei University of Education, Wuhan 430205, P. R. China

Abstract

Carbon nanodots (CDs) are fluorescent nanomaterials that have attracted widespread attention for biomedical applications. Since different cells possess different membrane potentials, the influence of the trans-membrane potential on the adsorption behaviour needs to be explored extensively. It was found that fewer CDs can be adsorbed by depolarized cells with decreased membrane potential, as compared to hyperpolarized cells with enhanced membrane potential that promotes the adsorption of CDs. A similar adsorption trend was observed for two cell lines, namely NIH3T3 and MCF-7. Since phosphocholine is the primary component of the cellular membrane, the adsorption behaviour of CDs is further complemented by the interactions between CDs and three kinds of liposomes with different surface charges via the thermodynamic and kinetic perspectives. The adsorption constants between negatively charged CDs and liposomes are positively related to their membrane potential, which is similar to the adsorption rate constants. It was further found that the adsorption of CDs onto liposomes was initiated by electrostatic attraction forces. The membrane integrity and fluidity of liposomes exposed to CDs have also been characterized. The membrane potential of cells is, therefore, particularly important when predicting the relative cytotoxicity of CDs and developing CDs-based nanomaterials for biomedical applications.

Graphical abstract: The adsorption behaviour of carbon nanodots modulated by cellular membrane potential

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C9EN00991D
Article type
Paper
Submitted
02 Sep 2019
Accepted
30 Dec 2019
First published
03 Jan 2020

Environ. Sci.: Nano, 2020,7, 880-890

Permissions

Request permissions

The adsorption behaviour of carbon nanodots modulated by cellular membrane potential

S. Zhang, C. Xiao, H. He, Z. Xu, B. Wang, X. Chen, C. Li, B. Jiang and Y. Liu, Environ. Sci.: Nano, 2020, 7, 880 DOI: 10.1039/C9EN00991D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements