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Brachychiton populneus as a novel source of bioactive ingredients with therapeutic effects: antioxidant, enzyme inhibitory, anti-inflammatory properties and LC–ESI-MS profile

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Abstract

Brachychiton populneus is one of the unexploited Tunisian plants, traditionally eaten as food and used for medicinal purposes. The present study aimed to investigate the phytochemical components of the seeds, leaves and flowers from B. populneus using three different solvents and to explore their antioxidant, anti-inflammatory and neuroprotective effects. Further, this study was focused on the identification of phenolic compounds from the most active extract. In vitro, all extracts showed strong antioxidant property by DPPH, ferrous ion chelating and lipid peroxidation-inhibiting assays, noticeable anti-inflammatory activity by protein denaturation and membrane stabilization methods and important neuroprotective effects by acetylcholinesterase inhibitory test. In vivo, B. populneus (50, 100 and 200 mg/kg, i.p.) showed significant dose–response anti-inflammatory effects against carrageenan-induced paw edema. With respect to the phenolic profile, the leaf methanol extract presented eight phenolic acids, one flavone and four flavonoids, with salvianolic acid B (820.3 mg/kg), caffeic acid (224.03 mg/kg), syringic acid (100.2 mg/kg) and trans-ferulic acid (60.02 mg/kg) as the major compounds. The results of the current study suggested that B. populneus could be a precious source of health-benefitting biomolecules and may be developed as new antioxidant, anti-inflammatory and AChE inhibitors.

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References

  • Abdel-Megeed A, Salem MZ, Ali HM, Gohar YM (2013) Brachychiton diversifolius as a source of natural products: antibacterial and antioxidant evaluation of extracts of wood branches. J Pure Appl Microbiol 7:1843–1850

    Google Scholar 

  • Abdul-Hafeez EY, Karamova NS, Ilinskaya ON (2014) Antioxidant activity and total phenolic compound content of certain medicinal plants. Int J Biosci 5:213–222

    Article  CAS  Google Scholar 

  • Altunkaya A, Gökmen V (2008) Effect of various inhibitors on enzymatic browning, antioxidant activity and total phenol content of fresh lettuce (Lactuca sativa). Food Chem 107:1173–1179

    Article  CAS  Google Scholar 

  • Batool R, Khan MR, Zai JA, Ali S, Maryam S, Naz I, Bibi S (2018) Brachychiton populneus (Schott & Endl.) R. Br. ameliorate carbon tetrachloride induced oxidative stress through regulation of endoplasmic reticulum stress markers and inflammatory mediators in Sprague–Dawley male rats. Biomed Pharmacother 107:1601–1610

    Article  PubMed  Google Scholar 

  • Bekir J, Mars M, Souchard JP, Bouajila J (2013) Assessment of antioxidant, anti-inflammatory, anti-cholinesterase and cytotoxic activities of pomegranate (Punica granatum) leaves. Food Chem Toxicol 55:470–475

    Article  CAS  PubMed  Google Scholar 

  • Bhatta S, Ratti C, Poubelle PE, Stevanovic T (2018) Nutrients, antioxidant capacity and safety of hot water extract from sugar maple (Acer saccharum M.) and red maple (Acer rubrum L.) bark. Plant Foods Hum Nutr 73:25–33

    Article  CAS  PubMed  Google Scholar 

  • Botsaris G, Orphanides A, Yiannakou E, Gekas V, Goulas V (2015) Antioxidant and antimicrobial effects of Pistacia lentiscus L. extracts in pork sausages. Food Technol Biotechnol 53:472

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Bufalo MC, Ferreira I, Costa G, Francisco V, Liberal J, Cruz MT, Sforcin JM (2013) Propolis and its constituent caffeic acid suppress LPS-stimulated pro-inflammatory response by blocking NF-kappaB and MAPK activation in macrophages. J Ethnopharmacol 149:84–92

    Article  CAS  PubMed  Google Scholar 

  • Carter P (1971) Spectrophotometric determination of serum iron at the submicrogram level with a new reagent (ferrozine). Anal Biochem 40:450–458

    Article  CAS  PubMed  Google Scholar 

  • Chahbani A, Fakhfakh N, Balti MA, Mabrouk M, El-Hatmi H, Zouari N, Kechaou N (2018) Microwave drying effects on drying kinetics, bioactive compounds and antioxidant activity of green peas (Pisum sativum L.). Food Biosci 25:32–38

    Article  CAS  Google Scholar 

  • Chan SW, Lee CY, Yap CF, Mustapha WAW, Ho CW (2009) Optimisation of extraction conditions for phenolic compounds from limau purut (Citrus hystrix) peels. Int Food Res J 16:203–213

    Google Scholar 

  • Chen HQ, Jin ZY, Wang XJ, Xu XM, Deng L, Zhao JW (2008) Luteolin protects dopaminergic neurons from inflammation-induced injury through inhibition of microglial activation. Neurosci Lett 448:175–179

    Article  CAS  PubMed  Google Scholar 

  • Chen T, Liu W, Chao X, Zhang L, Qu Y, Huo J, Fei Z (2011) Salvianolic acid B attenuates brain damage and inflammation after traumatic brain injury in mice. Brain Res Bull 84:163–168

    Article  CAS  PubMed  Google Scholar 

  • Chen PX, Zhang H, Marcone MF, Pauls KP, Liu R, Tang Y, Zhang B, Renaud JB, Tsao R (2017) Anti-inflammatory effects of phenolic-rich cranberry bean (Phaseolus vulgaris L.) extracts and enhanced cellular antioxidant enzyme activities in Caco-2 cells. J Funct Foods 38:675–685

    Article  CAS  Google Scholar 

  • Corina D, Delia M, Ersilia A, Claudia F, Istvan O, Andrea B, Oana C (2019) Phytochemical characterization and evaluation of the antimicrobial, antiproliferative and pro-apoptotic potential of Ephedra alata Decne. Hydroalcoholic extract against the MCF-7 breast cancer cell line. Molecules 24:13

    Google Scholar 

  • Elfalleh W, Kirkan B, Sarikurkcu C (2019) Antioxidant potential and phenolic composition of extracts from Stachys tmolea: an endemic plant from Turkey. Ind Crop Prod 127:212–216

    Article  CAS  Google Scholar 

  • Ellman GL, Courtney KD, Andres V Jr, Featherstone RM (1961) A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 7:88–95

    Article  CAS  PubMed  Google Scholar 

  • Fang N, Yu S, Prior RL (2002) LC/MS/MS characterization of phenolic constituents in dried plums. J Agric Food Chem 50:3579–3585

    Article  CAS  PubMed  Google Scholar 

  • Farag MA, Abou Zeid AH, Hamed MA, Kandeel Z, El-Rafie HM, El-Akad RH (2015) Metabolomic fingerprint classification of Brachychiton acerifolius organs via UPLC-qTOF-PDA-MS analysis and chemometrics. Nat Prod Res 29:116–124

    Article  CAS  PubMed  Google Scholar 

  • Ferrali M, Signorini C, Ciccoli L, Comporti M (1992) Iron release and membrane damage in erythrocytes exposed to oxidizing agents, phenylhydrazine, divicine and isouramil. Biochem J 285:295–301

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Figueroa JG, Borrás-Linares I, Lozano-Sánchez J, Segura-Carretero A (2018) Comprehensive characterization of phenolic and other polar compounds in the seed and seed coat of avocado by HPLC-DAD-ESI-QTOF-MS. Food Res Int 105:752–763

    Article  CAS  PubMed  Google Scholar 

  • Garcıa MD, Fernandez MA, Alvarez A, Saenz MT (2004) Antinociceptive and anti-inflammatory effect of the aqueous extract from leaves of Pimenta racemosa var. ozua (Mirtaceae). J Ethnopharmacol 91:69–73

    Article  PubMed  Google Scholar 

  • Gasmi A, Benabderrahim MA, Guasmi F, Elfalleh W, Triki T, Zammouri T, Ferchichi A (2019) Phenolic profiling, sugar composition and antioxidant capacity of arta (Calligonum comosum L.), a wild Tunisian desert plant. Ind Crop Prod 130:436–442

    Article  CAS  Google Scholar 

  • Goh CW, Aw CC, Lee JH, Chen CP, Browne ER (2011) Pharmacokinetic and pharmacodynamic properties of cholinesterase inhibitors donepezil, tacrine, and galantamine in aged and young Lister hooded rats. Drug Metab Dispos 39:402–411

    Article  CAS  PubMed  Google Scholar 

  • Gori A, Ferrini F, Marzano MC, Tattini M, Centritto M, Baratto MC, Pogni R, Brunetti C (2016) Characterisation and antioxidant activity of crude extract and polyphenolic rich fractions from C. incanus leaves. Int J Mol Sci 17:1344

    Article  CAS  PubMed Central  Google Scholar 

  • Ha SK, Moon E, Lee P, Ryu JH, Oh MS, Kim SY (2012) Acacetin attenuates neuroinflammation via regulation the response to LPS stimuli in vitro and in vivo. Neurochem Res 37:1560–1567

    Article  CAS  PubMed  Google Scholar 

  • Irawaty W, Ayucitra A (2018) Assessment on antioxidant and in vitro antidiabetes activities of different fractions of Citrus hystrix peel. Int Food Res J 25:2467–2477

    CAS  Google Scholar 

  • Jia Z, Tang M, Wu J (1999) The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64:555–559

    Article  Google Scholar 

  • Khan AS (2017) Antipyretic and analgesic activities of some economically important woody plants. In: Medicinally important trees. Springer, Cham, pp 159–185

  • Loram LC, Fuller A, Fick LG, Cartmell T, Poole S, Mitchell D (2007) Cytokine profiles during carrageenan-induced inflammatory hyperalgesia in rat muscle and hind paw. J Pain 8:127–136

    Article  CAS  PubMed  Google Scholar 

  • Mohammed A, Liman ML, Atiku MK (2013) Chemical composition of the methanolic leaf and stem bark extracts of Senna siamea Lam. J. Pharmacogn Phytother 5:98–100

    CAS  Google Scholar 

  • Mokbli S, Sbihi HM, Nehdi IA, Romdhani-Younes M, Tan CP, Al-Resayes SI (2018) A comparative study of Brachychiton populneus seed and seed-fiber oils in Tunisia. Waste Biomass Valorization 9:635–643

    Article  CAS  Google Scholar 

  • Oboh G, Agunloye OM, Akinyemi AJ, Ademiluyi AO, Adefegha SA (2013) Comparative study on the inhibitory effect of caffeic and chlorogenic acids on key enzymes linked to Alzheimer’s disease and some pro-oxidant induced oxidative stress in rats’ brain-in vitro. Neurochem Res 38:413–419

    Article  CAS  PubMed  Google Scholar 

  • Ojha D, Mukherjee H, Mondal S, Jena A, Dwivedi VP, Mondal KC, Malhotra B, Samanta A, Chattopadhyay D (2014) Anti-inflammatory activity of Odina wodier Roxb, an Indian folk remedy, through inhibition of toll-like receptor 4 signaling pathway. PLoS ONE 9:e104939

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Okpo SO, Fatokun F, Adeyemi OO (2001) Analgesic and anti-inflammatory activity of Crinum glaucum aqueous extract. J Ethnopharmacol 78:207–211

    Article  CAS  PubMed  Google Scholar 

  • Ooi KL, Muhammad TST, Tan ML, Sulaiman SF (2011) Cytotoxic, apoptotic and anti-α-glucosidase activities of 3,4-di-O-caffeoyl quinic acid, an antioxidant isolated from the polyphenolic-rich extract of Elephantopus mollis Kunth. J Ethnopharmacol 135:685–695

    Article  CAS  PubMed  Google Scholar 

  • Pandey KB, Rizvi SI (2009) Plant polyphenols as dietary antioxidants in human health and disease. Oxid Med Cell Longev 2:270–278

    Article  PubMed  PubMed Central  Google Scholar 

  • Parejo I, Jauregui O, Sánchez-Rabaneda F, Viladomat F, Bastida J, Codina C (2004) Separation and characterization of phenolic compounds in fennel (Foeniculum vulgare) using liquid chromatography–negative electrospray ionization tandem mass spectrometry. J Agric Food Chem 52:3679–3687

    Article  CAS  PubMed  Google Scholar 

  • Perez V, Chang ET (2014) Sodium-to-potassium ratio and blood pressure, hypertension, and related factors. Adv Nutr 5:712–741

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Rao KS, Jones GP, Rivett DE, Tucker DJ (1989) Fatty acid and amino acid compositions of Brachychiton discolor, Brachychiton diversifolius, and Brachychiton acerifolius seeds. J Agric Food Chem 37:916–917

    Article  CAS  Google Scholar 

  • Říha M, Karlíčková J, Filipský T, Macáková K, Rocha L, Bovicelli P, Mladěnka P (2014) In vitro evaluation of copper-chelating properties of flavonoids. RSC Adv 4:32628–32638

    Article  CAS  Google Scholar 

  • Rjeibi I, Feriani A, Ben Saad A, Sdayria J, Saidi I, Ncib S, Hfaiedh N (2018) Lycium europaeum extract: a new potential antioxidant source against cisplatin-induced liver and kidney injuries in mice. Oxid Med Cell Longev. https://doi.org/10.1155/2018/1630751

  • Ruiz-Ruiz JC, Matus-Basto AJ, Acereto-Escoffié P, Segura-Campos MR (2017) Antioxidant and anti-inflammatory activities of phenolic compounds isolated from Melipona beecheii honey. Food Agric Immunol 28:1424–1437

    Article  CAS  Google Scholar 

  • Sadique J, Al-Rqobahs WA, Bughaith EI, Gindi AR (1989) The bioactivity of certain medicinal plants on the stabilization of RBC membranesystem. Fitoterapia 60:525–532

    Google Scholar 

  • Salem MZM, Ali HM, Mansour MM (2014) Fatty acid methyl esters from air-dried wood, bark, and leaves of Brachychiton diversifolius R. Br: antibacterial, antifungal, and antioxidant activities. BioResources 9:3835–3845

    Google Scholar 

  • Seibert K, Zhang Y, Leahy K, Hauser S, Masferrer J, Perkins W, Lee L, Isakson P (1994) Pharmacological and biochemical demonstration of the role of cyclooxygenase 2 in inflammation and pain. Proc Natl Acad Sci 91:12013–12017

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Škerget M, Kotnik P, Hadolin M, Hraš AR, Simonič M, Knez Ž (2005) Phenols, proanthocyanidins, flavones and flavonols in some plant materials and their antioxidant activities. Food Chem 89:191–198

    Article  CAS  Google Scholar 

  • Sofowora A (1996) Medicinal plants and traditional medicine in Africa. Karthala, Paris, p 380

  • Su XY, Wang ZY, Liu JR (2009) In vitro and in vivo antioxidant activity of Pinus koraiensis seed extract containing phenolic compounds. Food Chem 117:681–686

    Article  CAS  Google Scholar 

  • Thabet AA, Youssef FS, El-Shazly M, El-Beshbishy HA, Singab ANB (2018) Validation of the antihyperglycaemic and hepatoprotective activity of the flavonoid rich fraction of Brachychiton rupestris using in vivo experimental models and molecular modelling. Food Chem Toxicol 114:302–310

    Article  CAS  PubMed  Google Scholar 

  • Tlili N, Kirkan B, Sarikurkcu C (2019) LC–ESI–MS/MS characterization, antioxidant power and inhibitory effects on α-amylase and tyrosinase of bioactive compounds from hulls of Amygdalus communis: the influence of the extracting solvents. Ind Crop Prod 128:147–152

    Article  CAS  Google Scholar 

  • Ullah HA, Zaman S, Juhara F, Akter L, Tareq SM, Masum EH, Bhattacharjee R (2014) Evaluation of antinociceptive, in-vivo & in-vitro anti-inflammatory activity of ethanolic extract of Curcuma zedoaria rhizome. BMC Compl Altern Med 14:346

    Article  Google Scholar 

  • Uysal S, Aumeeruddy-Elalfi Z, Zengin G, Aktumsek A, Mocan A, Custodio L, Soković M (2018) Insight into the biological properties and phytochemical composition of Ballota macrodonta Boiss. et Balansa,—an endemic medicinal plant from Turkey. Ind Crop Prod 113:422–428

    Article  CAS  Google Scholar 

  • Verma S, Singh A, Mishra A (2013) Gallic acid: molecular rival of cancer. Environ Toxicol Pharmacol 35:473–485

    Article  CAS  PubMed  Google Scholar 

  • Wolfe K, Wu X, Liu RH (2003) Antioxidant activity of apple peels. J Agric Food Chem 51:609–614

    Article  CAS  PubMed  Google Scholar 

  • Wu CT, Deng JS, Huang WC, Shieh PC, Chung MI, Huang GJ (2019) Salvianolic acid C against acetaminophen-induced acute liver injury by attenuating inflammation, oxidative stress, and apoptosis through inhibition of the Keap1/Nrf2/HO-1 signaling. Oxid Med Cell Longev. https://doi.org/10.1155/2019/9056845

  • Xu Q, Wang Y, Guo S, Shen Z, Wang Y, Yang L (2014) Anti-inflammatory and analgesic activity of aqueous extract of Flos populi. J Ethnopharmacol 152:540–545

    Article  PubMed  Google Scholar 

  • Yakoub ARB, Abdehedi O, Jridi M, Elfalleh W, Nasri M, Ferchichi A (2018) Flavonoids, phenols, antioxidant, and antimicrobial activities in various extracts from Tossa jute leave (Corchorus olitorus L.). Ind Crop Prod 118:206–213

    Article  CAS  Google Scholar 

  • Yıldırım A, Mavi A, Kara AA (2001) Determination of antioxidant and antimicrobial activities of Rumex crispus L. extracts. J Agric Food Chem 49:4083–4089

    Article  CAS  PubMed  Google Scholar 

  • Zeid AHA, Farag MA, Hamed MAA, Kandil ZAA, El-Akad RH, El-Rafie HM (2017) Flavonoid chemical composition and antidiabetic potential of Brachychiton acerifolius leaves extract. Asian Pac J Trop Biomed 7:389–396

    Article  Google Scholar 

  • Zhang Q, Hu XF, Xin MM, Liu HB, Sun LJ, Morris-Natschke SL, Lee KH (2018) Antidiabetic potential of the ethyl acetate extract of Physalis alkekengi and chemical constituents identified by HPLC-ESI-QTOF-MS. J Ethnopharmacol 225:202–210

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

The authors would like to thank Mr. Zied Tlili, a teacher of English for Specific Purposes at the Higher Institute of Business Administration of Gafsa, Tunisia, for high-quality language editing which significantly contributed to the completion of this work.

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Authors and Affiliations

  1. Research Unit of Macromolecular Biochemistry and Genetics, Faculty of Sciences of Gafsa, 2112, Gafsa, Tunisia

    Ilhem Rjeibi, Sami Souid & Najla Hfaiedh

  2. Faculty of Sciences of Gafsa, University of Gafsa, 2112, Gafsa, Tunisia

    Anouar Ben Saad

  3. Unit of Common Services, Faculty of Sciences Gafsa, University of Gafsa, 2112, Gafsa, Tunisia

    Sana Ncib

  4. Laboratory of Animal Ecophysiology, Faculty of Science of Sfax, University of Sfax, 3018, Sfax, Tunisia

    Mohamed Salah Allagui

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  1. Ilhem Rjeibi
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Rjeibi, I., Ben Saad, A., Ncib, S. et al. Brachychiton populneus as a novel source of bioactive ingredients with therapeutic effects: antioxidant, enzyme inhibitory, anti-inflammatory properties and LC–ESI-MS profile. Inflammopharmacol 28, 563–574 (2020). https://doi.org/10.1007/s10787-019-00672-8

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