Abstract
Main conclusion
Components of the photosynthetic electron transport chain in pea (Pisum sativum L.) leaves under in vivo conditions showed the following sensitivity to the inhibitory action of chromium(VI): intersystem electron transport > photosystem I > photosystem II.
Abstract
Inhibitory effects of chromium (VI) (K2Cr2O7, Cr) on the light reactions of photosynthesis were studied in vivo in Pisum sativum L. by using Multi-function Plant Efficiency Analyser (M-PEA-2). Photosynthetic parameters related to photosystem (PS) II, PSI and intersystem electron carriers were calculated from the light-induced kinetics of prompt chlorophyll a fluorescence (OJIP transient), delayed chlorophyll a fluorescence (DF), and 820 nm modulated reflection (MR). We showed that the I2 step of DF induction is sensitive to inhibition of the Q0 site of the cytochrome b6f complex. Such parameters as δRo of the JIP test related to the functional state of photosynthetic reactions beyond the PQ pool, Vred of the MR induction assigned to the overall rate of P700+ and plastocyanin reduction, and I2 step of the DF induction were significantly altered in the presence of low-dose Cr(VI). Moderate doses of Cr affected mainly PSI-related parameters including Vox and ΔMR parameters of the MR induction, whereas high-dose treatment influenced JIP test parameters φPo(= FV/FM) and ψEo related to PSII. The obtained results showed that the earliest Cr(VI) effect on the photosynthetic electron transport chain manifests itself by inhibition of the intersystem electron transport, rather, at the level of the cytochrome b6f complex. Inhibitory effects of Cr on PSI were more pronounced than those on PSII. Sensitivity of the used kinetic parameters toward the functional state of photosynthetic reactions makes this approach suitable for early diagnostics of toxic action of pollutants on plants.
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Abbreviations
- Chl:
-
Chlorophyll
- PS:
-
Photosystem
- PQ:
-
Plastoquinone
- QA and QB :
-
Primary and secondary electron acceptors in PSII
- PET:
-
Photosynthetic electron transport
- O, J, I, P:
-
Particular steps in the Chl fluorescence induction curve
- DF:
-
Delayed Chl fluorescence
- MR:
-
820 nm modulated reflection, M-PEA: Multi-function Plant Efficiency Analyser
- M-PEA:
-
Multi-function Plant Efficiency Analyser
- HM:
-
Heavy metal
- Cr:
-
Chromium
- PPFD:
-
Photosynthetic photon flux density
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Acknowledgements
The authors thank Dr. N. Seifullina for growing plants, A. Maslakov for assistance with modeling, and Dr. E. Tyystjarvi for help with manuscript preparation. The authors acknowledge the support from the Russian Foundation for Basic Research (projects 17-04-00676 and 19-04-00999).
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Todorenko, D., Timofeev, N., Kovalenko, I. et al. Chromium effects on photosynthetic electron transport in pea (Pisum sativum L.). Planta 251, 11 (2020). https://doi.org/10.1007/s00425-019-03304-1
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DOI: https://doi.org/10.1007/s00425-019-03304-1