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Skeletal muscle cell transplantation: models and methods

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Journal of Muscle Research and Cell Motility Aims and scope Submit manuscript

Abstract

Xenografts of skeletal muscle are used to study muscle repair and regeneration, mechanisms of muscular dystrophies, and potential cell therapies for musculoskeletal disorders. Typically, xenografting involves using an immunodeficient host that is pre-injured to create a niche for human cell engraftment. Cell type and method of delivery to muscle depend on the specific application, but can include myoblasts, satellite cells, induced pluripotent stem cells, mesangioblasts, immortalized muscle precursor cells, and other multipotent cell lines delivered locally or systemically. Some studies follow cell engraftment with interventions to enhance cell proliferation, migration, and differentiation into mature muscle fibers. Recently, several advances in xenografting human-derived muscle cells have been applied to study and treat Duchenne muscular dystrophy and Facioscapulohumeral muscular dystrophy. Here, we review the vast array of techniques available to aid researchers in designing future experiments aimed at creating robust muscle xenografts in rodent hosts.

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  1. Department of Physiology, University of Maryland School of Medicine, 655 W. Baltimore St., Baltimore, MD, 21201, USA

    Amber L. Mueller & Robert J. Bloch

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Mueller, A.L., Bloch, R.J. Skeletal muscle cell transplantation: models and methods. J Muscle Res Cell Motil 41, 297–311 (2020). https://doi.org/10.1007/s10974-019-09550-w

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