Abstract
Human ovarian cyst glycoproteins (HOC, cyst gps) isolated from pseudomucinous type of human ovarian cyst fluids is one of the richest and pioneer sources for studying biosynthesis, structures and functional roles of blood group ABH, Lea,b,x,y, sLea and sLex active glycoproteins. After 70+ years of exploration, four top highlights are shared. (i) an updated concept of glycotopes and their internal structures in cyst gps was composited; (ii) the unknown codes of new genes in secreted cyst gps were unlocked as Lex and Ley; (iii) recognition profiles of cyst glycans and a sialic acid-rich (18%) glycan with lectins and antibodies were shown. (iv) Co-expression of Blood Group A/ A-Leb/y and B/B-Leb/y active Glycotopes in the same glycan chains were isolated and illustrated. These are the most advanced achievements since 1980.
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Abbreviations
- HOC or cyst gp:
-
Human ovarian cyst glycoprotein
- RF:
-
Recognition factor
- Le a, b, x, y :
-
Lea,Leb, Lex, Ley
- sLe a, sLe x :
-
Sialyl Lea and Sialyl Lex
- GBP(CBP):
-
Glycan (Carbohydrate) binding Protein
- SA:
-
Sialic acid
- GP or gp:
-
Glycoprotein
- ELLSA and ELLSIA:
-
Enzyme-linked lectinosorbent assay; and inhibition assay
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Acknowledgements
This work was supported by grants from the Chang Gung Medical Research plan (CMRPD No. 180482 and BMR 0008) Kwei-san, Tao-yuan, Taiwan, and the National Science Council (NSC 97-2628-B-182-002-MY3 and 97-2320-B-182-020-MY3) Taipei, Taiwan.
The author would like to thanks Drs. Khoo KH and Yu S. Y. for their long term contributions in the field of structural identification. Yang Z MD’s assistance to make the data analyzed and organized.
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This review is dedicated to the memory of Dr. E.A. Kabat (Columbia University College of Physicians and Surgeons, New York, USA) and Dr. W.M. Watkins (Royal Postgradunte Medical School, London, UK), who were pioneers in the field of human blood active glycotopes.
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Wu, A.M. Glycan structures and their recognition roles in the human blood group ABH/Ii, Lea, b, x, y and Sialyl Lea,x active cyst glycoproteins. Glycoconj J 36, 495–507 (2019). https://doi.org/10.1007/s10719-019-09887-x
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DOI: https://doi.org/10.1007/s10719-019-09887-x