Abstract
Mitogen-activated protein kinase-associated protein 1 (MAPKAP1) is a unique component of the mechanistic target of rapamycin (MTOR) pathway which plays a pivotal role in carcinogenesis. The role of enhancer variant in carcinogenesis receives increased attentions. However, the significance of enhancer variants of MAPKAP1 in glioma has not yet been investigated. The associations of enhancer variants of MAPKAP1 with glioma susceptibility were evaluated in a cohort of 400 glioma patients and 651 controls. The function of glioma susceptibility locus was examined by a set of biochemical assays. We found that an enhancer variant of MAPKAP1 rs473426 was associated with a significantly increased risk of glioma in a dominant manner (OR 1.53, 95% CI 1.13–2.06; P = 0.006). The association for rs1339499 located in the same enhancer approached the borderline of significance after multiple testing correction (OR 0.74, 95% CI 0.56–0.98; P = 0.037). Furthermore, cumulative associations of rs473426 and rs1339499 with glioma risk were observed (P = 0.011). Functional analyses showed that the risk allele rs473426 C downregulated the regulatory activity of enhancer by reducing the binding affinity of a transcriptional activator NFΙC, which resulted in lower gene expression both in vitro and in vivo. These results demonstrate for the first time that enhancer variant of MAPKAP1 confers susceptibility to glioma by downregulation of MAPKAP1 expression, and provide further evidence highlighting MAPKAP1 as a cancer suppressor in glioma carcinogenesis.
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Abbreviations
- MAPKAP1:
-
Mitogen-activated protein kinase-associated protein 1
- MTOR:
-
Mechanistic target of rapamycin
- GWAS:
-
Genome-wide association study
- MTORC2:
-
MTOR complex 2
- CHS:
-
Southern Han Chinese
- MAF:
-
Minor allelic frequency
- LD:
-
Linkage disequilibrium
- EMSA:
-
Electrophoretic mobility-shift assay
- ChIP:
-
Chromatin immunoprecipitation assay
- OR:
-
Odds ratio
- CI:
-
Confidence interval
References
1000 Genomes Project Consortium et al (2015) A global reference for human genetic variation. Nature 526:68
Adel Fahmideh M, Schwartzbaum J, Frumento P, Feychting M (2014) Association between DNA repair gene polymorphisms and risk of glioma: a systematic review and meta-analysis. Neuro Oncol 16:807–814
Auton A et al (2015) A global reference for human genetic variation. Nature 526:68–74
Barrett JC (2009) Haploview: visualization and analysis of SNP genotype data. Cold Spring Harb Protoc. https://doi.org/10.1101/pdb.ip71
Barrett T et al (2013) NCBI GEO: archive for functional genomics data sets—update. Nucleic Acids Res 41:D991–995
Cameron AJ, Linch MD, Saurin AT, Escribano C, Parker PJ (2011) mTORC2 targets AGC kinases through Sin1-dependent recruitment. Biochem J 439:287–297
Cheng J, Zhang D, Kim K, Zhao Y, Zhao Y, Su B (2005) Mip1, an MEKK2-interacting protein, controls MEKK2 dimerization and activation. Mol Cell Biol 25:5955–5964
Flicek P et al (2014) Ensembl 2014. Nucleic Acids Res 42:D749–755
Frias MA, Thoreen CC, Jaffe JD, Schroder W, Sculley T, Carr SA, Sabatini DM (2006) mSin1 is necessary for Akt/PKB phosphorylation, and its isoforms define three distinct mTORC2s. Curr Biol 16:1865–1870
Guertin DA, Sabatini DM (2007) Defining the role of mTOR in cancer. Cancer Cell 12:9–22
Guertin DA et al (2009) mTOR complex 2 is required for the development of prostate cancer induced by Pten loss in mice. Cancer Cell 15:148–159
Hernandez DG et al (2012) Integration of GWAS SNPs and tissue specific expression profiling reveal discrete eQTLs for human traits in blood and brain. Neurobiol Dis 47:20–28
Herz HM (2016) Enhancer deregulation in cancer and other diseases. BioEssays 38:1003–1015
Hietakangas V, Cohen SM (2008) TOR complex 2 is needed for cell cycle progression and anchorage-independent growth of MCF7 and PC3 tumor cells. BMC Cancer 8:282
Hu Z, Wang Y, Wang Y, Zang B, Hui H, You Z, Wang X (2017) Epigenetic activation of SIN1 promotes NSCLC cell proliferation and metastasis by affecting the epithelial-mesenchymal transition. Biochem Biophys Res Commun 483:645–651
Huang L, Xu W, Yan D, Dai L, Shi X (2016) Identification of expression quantitative trait loci of RPTOR for susceptibility to glioma. Tumour Biol 37:2305–2311
Inoki K, Corradetti MN, Guan KL (2005) Dysregulation of the TSC-mTOR pathway in human disease. Nat Genet 37:19–24
International HapMap Consortium (2003) The International HapMap Project. Nature 426:789–796
Jacinto E et al (2006) SIN1/MIP1 maintains rictor-mTOR complex integrity and regulates Akt phosphorylation and substrate specificity. Cell 127:125–137
Laplante M, Sabatini DM (2012) mTOR signaling in growth control and disease. Cell 149:274–293
Lapointe S, Perry A, Butowski NA (2018) Primary brain tumours in adults. Lancet 392:432–446
Liu P et al (2015) PtdIns(3,4,5)P3-dependent activation of the mTORC2 kinase complex. Cancer Discov 5:1194–1209
Louis DN et al (2007) The 2007 WHO classification of tumours of the central nervous system. Acta Neuropathol 114:97–109
Masri J, Bernath A, Martin J, Jo OD, Vartanian R, Funk A, Gera J (2007) mTORC2 activity is elevated in gliomas and promotes growth and cell motility via overexpression of rictor. Cancer Res 67:11712–11720
Masui K et al (2013) mTOR complex 2 controls glycolytic metabolism in glioblastoma through FoxO acetylation and upregulation of c-Myc. Cell Metab 18:726–739
McNamara S (2012) Treatment of primary brain tumours in adults. Nurs Stand 27:42–47
Ostrom QT, Gittleman H, Stetson L, Virk S, Barnholtz-Sloan JS (2018) Epidemiology of intracranial gliomas. Prog Neurol Surg 30:1–11
Paten B, Herrero J, Beal K, Fitzgerald S, Birney E (2008) Enredo and Pecan: genome-wide mammalian consistency-based multiple alignment with paralogs. Genome Res 18:1814–1828
Schroder WA, Buck M, Cloonan N, Hancock JF, Suhrbier A, Sculley T, Bushell G (2007) Human Sin1 contains Ras-binding and pleckstrin homology domains and suppresses Ras signalling. Cell Signal 19:1279–1289
Tatebe H et al (2017) Substrate specificity of TOR complex 2 is determined by a ubiquitin-fold domain of the Sin1 subunit. eLife 6:10
Visel A, Minovitsky S, Dubchak I, Pennacchio LA (2007) VISTA Enhancer Browser–a database of tissue-specific human enhancers. Nucleic Acids Res 35:D88–92
Wang D, Wu P, Wang H, Zhu L, Zhao W, Lu Y (2016) SIN1 promotes the proliferation and migration of breast cancer cells by Akt activation. Biosci Rep 36:e00424
Wenzelides S, Altmann H, Wendler W, Winnacker EL (1996) CTF5–a new transcriptional activator of the NFI/CTF family. Nucleic Acids Res 24:2416–2421
Xiao Q et al (2014) 9q33.3, a stress-related chromosome region, contributes to reducing lung squamous cell carcinoma risk. J Thorac Oncol 9:1041–1047
Xu J, Li X, Yang H, Chang R, Kong C, Yang L (2013) SIN1 promotes invasion and metastasis of hepatocellular carcinoma by facilitating epithelial-mesenchymal transition. Cancer 119:2247–2257
Yang Q, Inoki K, Ikenoue T, Guan KL (2006) Identification of Sin1 as an essential TORC2 component required for complex formation and kinase activity. Genes Dev 20:2820–2832
Yang HC, Lin CW, Chen CW, Chen JJ (2014) Applying genome-wide gene-based expression quantitative trait locus mapping to study population ancestry and pharmacogenetics. BMC Genomics 15:319
Yang G, Murashige DS, Humphrey SJ, James DE (2015) A positive feedback loop between Akt and mTORC2 via SIN1 phosphorylation. Cell Rep 12:937–943
Zoncu R, Efeyan A, Sabatini DM (2011) mTOR: from growth signal integration to cancer, diabetes and ageing. Nat Rev Mol Cell Biol 12:21–35
Funding
This work was funded by National Natural Science Foundation, P.R.C (Grant Number 81301772); Joint Funds for the Innovation of Science and Technology of Fujian province, P.R.C (Grant Number 2016Y9016); Natural Science Foundation of Fujian Province, P.R.C (Grant Number 2014J05087); and Startup Fund for Scientific Research of Fujian Medical University (Grant Number 2017XQ1085).
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LMH, WSX, DFY, XY, XS, SZ, HLH and LD participated in the study design. LMH, WSX, DFY, XY, SZ, HLH and LD performed the experiment. LMH, WSX, DFY, XY, XS, SZ and LD were involved in data collection and data interpretation. LMH, WSX, DFY, XY and LD participated in the statistical analyses. LMH and LD wrote the manuscript. All authors read and approved the final manuscript.
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Huang, L., Xu, W., Yan, D. et al. Genetic Predisposition to Glioma Mediated by a MAPKAP1 Enhancer Variant. Cell Mol Neurobiol 40, 643–652 (2020). https://doi.org/10.1007/s10571-019-00763-8
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DOI: https://doi.org/10.1007/s10571-019-00763-8