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Molecular mapping of major QTL conferring resistance to orange wheat blossom midge (Sitodiplosis mosellana) in Chinese wheat varieties with selective populations

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Abstract

Key message

Two novel midge resistance QTL were mapped to a 4.9-Mb interval on chromosome arm 4AL based on the genetic maps constructed with SNP markers.

Abstract

Orange wheat blossom midge (OWBM) is a devastating insect pest affecting wheat production. In order to detect OWBM resistance genes and quantitative trait loci (QTL) for wheat breeding, two recombinant inbred line (RIL) populations were established and used for molecular mapping. A total of seven QTL were detected on chromosomes 2D, 4A, 4D and 7D, respectively, of which positive alleles were all from the resistant parents except for the QTL on 7D. Two stable QTL (QSm.hbau-4A.2-1 and QSm.hbau-4A.2-2) were detected in both populations with the LOD scores ranging from 5.58 to 29.22 under all three environments, and they explained a combined phenotypic variation of 24.4–44.8%. These two novel QTL were mapped to a 4.9-Mb physical interval. The single-nucleotide polymorphism (SNP) markers AX-109543456, AX-108942696 and AX-110928325 were closely linked to the QTL and could be used for marker-assisted selection (MAS) for OWBM resistance in wheat breeding programs.

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Abbreviations

ANOVA:

Analysis of variance

EST:

Expressed sequence tag

ICIM:

Inclusive composite interval mapping

KASP:

Kompetitive allele specific PCR

LOD:

Logarithm of odds

MAS:

Marker-assisted selection

NIL:

Near-isogenic line

QTL:

Quantitative trait loci

RIL:

Recombinant inbred line

SNP:

Single-nucleotide polymorphism

SSR:

Simple sequence repeat

OWBM:

Orange wheat blossom midge

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Acknowledgements

We are grateful to Prof. Xinming Yang (Institute of Crop Sciences, Chinese Academy of Agricultural Sciences) for supplying the pedigree of some wheat varieties, Dr. Guangyao Zhao (Institute of Crop Sciences, Chinese Academy of Agricultural Sciences) for his advice in comparative genomic analysis, Prof. Zhengang Qu (Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences) for his advice in the identification of OWBM resistance, Dr. Yanru Cui (Hebei Agricultural University) for her help in selective population analysis and her careful review for this paper.

Funding

This work was supported by National Natural Science Foundation of China (31371617).

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Authors and Affiliations

  1. North China Key Laboratory for Crop Germplasm Resources of Education Ministry, College of Agronomy, Hebei Agricultural University, Baoding, 071000, Hebei, People’s Republic of China

    Lijing Zhang, Miaomiao Geng, Zhe Zhang, Yue Zhang, Shumin Wen, Guiru Liu & Ruihui Wang

  2. School of Agriculture and Environment, Faculty of Science, and the Institute of Agriculture, The University of Western Australia, Perth, 6009, Australia

    Guijun Yan

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  1. Lijing Zhang
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  2. Miaomiao Geng
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  3. Zhe Zhang
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  4. Yue Zhang
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  5. Guijun Yan
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  6. Shumin Wen
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  7. Guiru Liu
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Contributions

LZ and RW designed and conducted the study. MG, GY, SW and GL provided advice to the authors. SW, GL and RW performed RIL population construction. GL and RW performed OWBM invasion treatment and analyzed resistance index. LZ and ZZ performed experimental material collection for SNP genotyping. LZ, ZZ and YZ performed resistance evaluation.

Corresponding authors

Correspondence to Guiru Liu or Ruihui Wang.

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All research conducted in relation to this publication is in compliance with ethical standards. The authors declare that this study was performed and reported in accordance with the ethical standards of scientific conduct.

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Communicated by Steven S. Xu.

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Zhang, L., Geng, M., Zhang, Z. et al. Molecular mapping of major QTL conferring resistance to orange wheat blossom midge (Sitodiplosis mosellana) in Chinese wheat varieties with selective populations. Theor Appl Genet 133, 491–502 (2020). https://doi.org/10.1007/s00122-019-03480-4

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