Abstract
This study investigated the protective effect of Kaempferol against CdCl2-induced hippocampal damage and memory deficit in rats and investigated if such effects involve modulating the activity of AMPK/PTEN/Akt/mTOR axis. Adult male rats (n = 12/group) were divided into control or CdCl2-treated rats received the vehicle of Kaempferol for consecutive 6 weeks. Also, hippocampal cells were treated with CdCl2 in the presence or absence of Kaempferol for 24 h with or without 1 h pre-incubation with compound C, an AMPK inhibitor or with bpV a PTEN inhibitor. Kaempferol improved the behavioral of CdCl2-treated rats, preserved hippocampus structure and reduced hippocampal levels of ROS and protein levels of Bax and cleaved caspase-3. In both control and CdCl2-treated rats, Kaempferol significantly increased hippocampal levels of GSH levels and protein levels of Nfr2, Bcl2 and synaptic proteins (SNAP-25, PSD-25, and synapsin). Concomitantly, it increased the activity of PTEN and AMPK and subsequently, decreased the activity of Akt and mTOR. In cultured cells, individual pharmacological inhibition of PTEN by bpv or AMPK of compound C (CC) partially prevented the stimulatory effect of Kaempferol on Akt/mTOR and its inhibitory effect on cell death whereas a combination of both inhibitors completely prevented this. Also, inhibition of PTEN alone completely abolished the inhibitory effect of Kaempferol by synaptic proteins, whereas inhibition of AMPK completely abolished its stimulatory effect of Nfr2. In conclusion, Kaempferol protects against CdCl2-induced memory deficits and hippocampal apoptosis by its antioxidant potential and inhibition of Akt/mTOR axis and requires the activation of PTEN and AMPK.
Similar content being viewed by others
References
Shati AA, Alfaifi MY (2019) Trans-resveratrol inhibits tau phosphorylation in the brains of control and cadmium chloride-treated rats by activating PP2A and PI3K/Akt induced-inhibition of GSK3β. Neurochem Res 44:357–373. https://doi.org/10.1007/s11064-018-2683-8
Cao Y, Chen A, Radcliffe J et al (2009) Postnatal cadmium exposure, neurodevelopment, and blood pressure in children at 2, 5, and 7 years of age. Environ Health Perspect 117:1580–1586. https://doi.org/10.1289/ehp.0900765
Siu ER, Mruk DD, Porto CS, Cheng CY (2009) Cadmium-induced testicular injury. Toxicol Appl Pharmacol 238:240–249
Chen L, Xu B, Liu L et al (2011) Cadmium induction of reactive oxygen species activates the mTOR pathway, leading to neuronal cell death. Free Radic Biol Med 50:624–632. https://doi.org/10.1016/j.freeradbiomed.2010.12.032
Bai X, Jiang Y (2010) Key factors in mTOR regulation. Cell Mol Life Sci 67:239–253
Polak P, Hall MN (2009) mTOR and the control of whole body metabolism. Curr Opin Cell Biol 21:209–218
Yuan Y, Wang Y, Hu FF et al (2016) Cadmium activates reactive oxygen species-dependent AKT/mTOR and mitochondrial apoptotic pathways in neuronal cells. Biomed Environ Sci 29:117–126. https://doi.org/10.3967/bes2016.013
Spencer JPE (2010) The impact of fruit flavonoids on memory and cognition. Br J Nutr 104(Suppl 3):S40–S47
Yu L, Chen C, Wang LF et al (2013) Neuroprotective effect of kaempferol glycosides against brain injury and neuroinflammation by inhibiting the activation of NF-κB and STAT3 in transient focal stroke. PLoS ONE 8:e55839. https://doi.org/10.1371/journal.pone.0055839
Alkhalidy H, Moore W, Wang A et al (2018) Kaempferol ameliorates hyperglycemia through suppressing hepatic gluconeogenesis and enhancing hepatic insulin sensitivity in diet-induced obese mice. J Nutr Biochem 58:90–101. https://doi.org/10.1016/j.jnutbio.2018.04.014
Abdel-Aleem GA, Khaleel EF (2018) Rutin hydrate ameliorates cadmium chloride-induced spatial memory loss and neural apoptosis in rats by enhancing levels of acetylcholine, inhibiting JNK and ERK1/2 activation and activating mTOR signalling. Arch Physiol Biochem 124:367–377. https://doi.org/10.1080/13813455.2017.1411370
Morris R (1984) Developments of a water-maze procedure for studying spatial learning in the rat. J Neurosci Methods 11:47–60. https://doi.org/10.1016/0165-0270(84)90007-4
Al Dera H, Alassiri M, Eleawa SM et al (2019) Melatonin improves memory deficits in rats with cerebral hypoperfusion, possibly, through decreasing the expression of small-conductance Ca2+-activated K+ channels. Neurochem Res.https://doi.org/10.1007/s11064-019-02820-6
Seibenhener ML, Wooten MW (2012) Isolation and culture of hippocampal neurons from prenatal mice. J Vis Exp. https://doi.org/10.3791/3634
Arun RP, Sivanesan D, Vidyasekar P, Verma RS (2017) PTEN/FOXO3/AKT pathway regulates cell death and mediates morphogenetic differentiation of colorectal cancer cells under simulated microgravity. Sci Rep. https://doi.org/10.1038/s41598-017-06416-4
Xu J, Wu L, Zhang Y et al (2017) Activation of AMPK by OSU53 protects spinal cord neurons from oxidative stress. Oncotarget. https://doi.org/10.18632/oncotarget.22055
Horobin RW (2013) How histological stains work. Bancroft’s theory and practice of histological techniques. Elsevier, Amsterdam, pp 157–171
Hwang DF, Wang LC (2001) Effect of taurine on toxicity of cadmium in rats. Toxicology 167:173–180. https://doi.org/10.1016/S0300-483X(01)00472-3
Amara S, Abdelmelek H, Garrel C et al (2008) Preventive effect of zinc against cadmium-induced oxidative stress in the rat testis. J Reprod Dev 54:129–134. https://doi.org/10.1262/jrd.18110
Nwokocha CR, Nwokocha MI, Owu DU et al (2012) Comparative analysis on the effect of palm oil (Elaeis guineensis) in reducing cadmium and lead accumulation in liver of Wistar rats. Pharmacognosy Res 4:214–218. https://doi.org/10.4103/0974-8490.102266
Wang Q, Zhu J, Zhang K et al (2013) Induction of cytoprotective autophagy in PC-12 cells by cadmium. Biochem Biophys Res Commun 438:186–192. https://doi.org/10.1016/j.bbrc.2013.07.050
Chen L, Liu L, Huang S (2008) Cadmium activates the mitogen-activated protein kinase (MAPK) pathway via induction of reactive oxygen species and inhibition of protein phosphatases 2A and 5. Free Radic Biol Med 45:1035–1044. https://doi.org/10.1016/j.freeradbiomed.2008.07.011
Afifi O, Embaby A (2016) Histological study on the protective role of ascorbic acid on cadmium induced cerebral cortical neurotoxicity in adult male albino rats. J Microsc Ultrastruct 4:36. https://doi.org/10.1016/j.jmau.2015.10.001
Isaev NK, Avilkina S, Golyshev SA et al (2018) N-acetyl-L-cysteine and Mn2 + attenuate Cd2+-induced disturbance of the intracellular free calcium homeostasis in cultured cerebellar granule neurons. Toxicology 393:1–8. https://doi.org/10.1016/j.tox.2017.10.017
Sobaniec-Lotowska ME (2001) Ultrastructure of Purkinje cell perikarya and their dendritic processes in the rat cerebellar cortex in experimental encephalopathy induced by chronic application of valproate. Int J Exp Pathol 82:337–348. https://doi.org/10.1046/j.1365-2613.2001.00206.x
Carageorgiou H, Tzotzes V, Pantos C et al (2004) In vivo and in vitro effects of cadmium on adult rat brain total antioxidant status, acetylcholinesterase, (Na+,K+)-ATPase and Mg2+-ATPase activities: protection by l-cysteine. Basic Clin Pharmacol Toxicol 94:112–118. https://doi.org/10.1111/j.1742-7843.2004.pto940303.x
Panickar KS, Noremberg MD (2005) Astrocytes in cerebral ischemic injury: morphological and general considerations. Glia 50:287–298
Kouhestani S, Jafari A, Babaei P (2018) Kaempferol attenuates cognitive deficit via regulating oxidative stress and neuroinflammation in an ovariectomized rat model of sporadic dementia. Neural Regen Res 13:1827–1832. https://doi.org/10.4103/1673-5374.238714
Chitturi J, Santhakumar V, Kannurpatti SS (2019) Beneficial effects of kaempferol after developmental traumatic brain injury is through protection of mitochondrial function, oxidative metabolism, and neural viability. J Neurotrauma 36:1264–1278. https://doi.org/10.1089/neu.2018.6100
Yuan Y, Jiang CY, Xu H et al (2013) Cadmium-induced apoptosis in primary rat cerebral cortical neurons culture is mediated by a calcium signaling pathway. PLoS One 8:e64330. https://doi.org/10.1371/journal.pone.0064330
Jiang C, Yuan Y, Hu F et al (2014) Cadmium induces PC12 cells apoptosis via an extracellular signal-regulated kinase and c-Jun N-terminal kinase-mediated mitochondrial apoptotic pathway. Biol Trace Elem Res 158:249–258. https://doi.org/10.1007/s12011-014-9918-6
Zhu G, Liu X, Li H et al (2018) Kaempferol inhibits proliferation, migration, and invasion of liver cancer HepG2 cells by down-regulation of microRNA-21. Int J Immunopathol Pharmacol.https://doi.org/10.1177/2058738418814341
Kashafi E, Moradzadeh M, Mohamadkhani A, Erfanian S (2017) Kaempferol increases apoptosis in human cervical cancer HeLa cells via PI3K/AKT and telomerase pathways. Biomed Pharmacother 89:573–577. https://doi.org/10.1016/j.biopha.2017.02.061
Chin HK, Horng CT, Liu YS et al (2018) Kaempferol inhibits angiogenic ability by targeting VEGF receptor-2 and downregulating the PI3K/AKT, MEK and ERK pathways in VEGF-stimulated human umbilical vein endothelial cells. Oncol Rep 39:2351–2357. https://doi.org/10.3892/or.2018.6312
Han B, Yu YQ, Yang QL et al (2017) Kaempferol induces autophagic cell death of hepatocellular carcinoma cells via activating AMPK signaling. Oncotarget 8:86227–86239. https://doi.org/10.18632/oncotarget.21043
Itoh K, Tong KI, Yamamoto M (2004) Molecular mechanism activating Nrf2-Keap1 pathway in regulation of adaptive response to electrophiles. Free Radic Biol Med 36:1208–1213
Suzuki T, Yamamoto M (2015) Molecular basis of the Keap1-Nrf2 system. Free Radic Biol Med 88:93–100
Joo MS, Kim WD, Lee KY et al (2016) AMPK facilitates nuclear accumulation of Nrf2 by phosphorylating at serine 550. Mol Cell Biol 36:1931–1942. https://doi.org/10.1128/mcb.00118-16
Zimmermann K, Baldinger J, Mayerhofer B et al (2015) Activated AMPK boosts the Nrf2/HO-1 signaling axis—a role for the unfolded protein response. Free Radic Biol Med 88:417–426. https://doi.org/10.1016/j.freeradbiomed.2015.03.030
Sperow M, Berry RB, Bayazitov IT et al (2012) Phosphatase and tensin homologue (PTEN) regulates synaptic plasticity independently of its effect on neuronal morphology and migration. J Physiol 590:777–792. https://doi.org/10.1113/jphysiol.2011.220236
Butler MG, Dazouki MJ, Zhou XP et al (2005) Subset of individuals with autism spectrum disorders and extreme macrocephaly associated with germline PTEN tumour suppressor gene mutations. J Med Genet 42:318–321. https://doi.org/10.1136/jmg.2004.024646
Endersby R, Baker SJ (2008) PTEN signaling in brain: neuropathology and tumorigenesis. Oncogene 27:5416–5430
Goffin A, Hoefsloot LH, Bosgoed E et al (2001) PTEN mutation in a family with Cowden syndrome and autism. Am J Med Genet - Neuropsychiatr Genet 105:521–524. https://doi.org/10.1002/ajmg.1477
Jurado S, Benoist M, Lario A et al (2010) PTEN is recruited to the postsynaptic terminal for NMDA receptor-dependent long-term depression. EMBO J 29:2827–2840. https://doi.org/10.1038/emboj.2010.160
Harrison FE, Hosseini AH, McDonald MP (2009) Endogenous anxiety and stress responses in water maze and Barnes maze spatial memory tasks. Behav Brain Res 198:247–251. https://doi.org/10.1016/j.bbr.2008.10.015
Wang B, Du Y (2013) Cadmium and its neurotoxic effects. Oxid Med Cell Longev. https://doi.org/10.1155/2013/898034
Acknowledgements
All authors extend their appreciation to the deanship of Scientific Research at King Khalid University, Abha, KSA for funding this work through the research groups program under Grant Number (R.G.P.1/46/40). They also would like to thank their technical staff at the animal house for taking care of the animals and helping in tissue dissection.
Funding
This study was funded by the Deanship of Scientific Research at King Khalid University, Abha, Saudi Arabia (R.G.P.1/46/40). Also, this research was funded by Deanship of Scientific Research at Princess Nourah Bint Abdulrahman University through the Fast-track Research Funding Program.
Author information
Authors and Affiliations
Contributions
AE and MBM proposed the study and obtained the fund. AE, SME, HD designed the experimental procedure. AE, HD, MA performed the experimental procedure and analyzed the data. AE and HD wrote the initial draft and SME finalized the final version.
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations
Rights and permissions
About this article
Cite this article
El-kott, A.F., Bin-Meferij, M.M., Eleawa, S.M. et al. Kaempferol Protects Against Cadmium Chloride-Induced Memory Loss and Hippocampal Apoptosis by Increased Intracellular Glutathione Stores and Activation of PTEN/AMPK Induced Inhibition of Akt/mTOR Signaling. Neurochem Res 45, 295–309 (2020). https://doi.org/10.1007/s11064-019-02911-4
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11064-019-02911-4