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Stark fluorescence spectroscopy on peridinin–chlorophyll–protein complex of dinoflagellate, Amphidinium carterae

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Abstract

Because of their peculiar but intriguing photophysical properties, peridinin–chlorophyll–protein complexes (PCPs), the peripheral light-harvesting antenna complexes of photosynthetic dinoflagellates have been unique targets of multidimensional theoretical and experimental investigations over the last few decades. The major light-harvesting chlorophyll a (Chl a) pigments of PCP are hypothesized to be spectroscopically heterogeneous. To study the spectral heterogeneity in terms of electrostatic parameters, we, in this study, implemented Stark fluorescence spectroscopy on PCP isolated from the dinoflagellate Amphidinium carterae. The comprehensive theoretical modeling of the Stark fluorescence spectrum with the help of the conventional Liptay formalism revealed the simultaneous presence of three emission bands in the fluorescence spectrum of PCP recorded upon excitation of peridinin. The three emission bands are found to possess different sets of electrostatic parameters with essentially increasing magnitude of charge-transfer character from the blue to redder ones. The different magnitudes of electrostatic parameters give good support to the earlier proposition that the spectral heterogeneity in PCP results from emissive Chl a clusters anchored at a different sites and domains within the protein network.

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Acknowledgements

Md. W., A. M. A., and R. v. G. were supported by the VU University Amsterdam, the Laserlab-Europe Consortium and the advanced investigator Grant (267333, PHOTPROT) from the European Research Council. Md. W. and R. v. G. were supported further by the TOP grant (700.58.305) from the Foundation of Chemical Sciences part of NWO.

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Authors and Affiliations

  1. Biophysics of Photosynthesis, Department of Physics and Astronomy, Faculty of Sciences, VU University Amsterdam, Amsterdam, The Netherlands

    Anjue Mane Ara, Rienk van Grondelle & Md. Wahadoszamen

  2. Department of Physics, Jagannath University, Dhaka, 1100, Bangladesh

    Anjue Mane Ara & Md. Shakil Bin Kashem

  3. Department of Physics, University of Dhaka, Dhaka, 1000, Bangladesh

    Md. Wahadoszamen

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  1. Anjue Mane Ara
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  2. Md. Shakil Bin Kashem
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  3. Rienk van Grondelle
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Correspondence to Md. Wahadoszamen.

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Ara, A.M., Shakil Bin Kashem, M., van Grondelle, R. et al. Stark fluorescence spectroscopy on peridinin–chlorophyll–protein complex of dinoflagellate, Amphidinium carterae. Photosynth Res 143, 233–239 (2020). https://doi.org/10.1007/s11120-019-00688-9

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