Abstract
Passiflora edulis, the yellow passion fruit, is the main crop from the Passiflora genus, which comprises 525 species with its diversity center in South America. Genetic maps and a BAC (bacterial artificial chromosome) genomic library are available, but the nine chromosome pairs of similar size and morphology (2n = 18) hamper chromosome identification, leading to different proposed karyotypes. Thus, the aim of this study was to establish chromosome-specific markers for the yellow passion fruit using single-copy and repetitive sequences as probes in fluorescent in situ hybridizations (FISH) to allow chromosome identification and future integration with whole genome data. Thirty-six BAC clones harboring genes and three retrotransposons (Ty1-copy, Ty3-gypsy, and LINE) were selected. Twelve BACs exhibited a dispersed pattern similar to that revealed by retroelements, and one exhibited subtelomeric distribution. Twelve clones showed unique signals in terminal or subterminal regions of the chromosomes, allowing their genes to be anchored to six chromosome pairs that can be identified with single-copy markers. The markers developed herein will provide an important tool for genomic and evolutionary studies in the Passiflora genus.
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19 May 2020
The article “Identification of passion fruit (<Emphasis Type="Italic">Passiflora edulis</Emphasis>) chromosomes using BAC-FISH,” written by Mariela A. Sader, Yhanndra Dias, Zirlane P. da Costa, Carla Munhoz, Helen Penha, Hélène Bergès, Maria Lucia C. Vieira, and Andrea Pedrosa-Harand, was originally published with incorrect values in Figure 4.
Abbreviations
- BACs:
-
Bacterial artificial chromosomes
- FISH:
-
Fluorescent in situ hybridizations
- NORs:
-
Active nucleolar organizer regions
- CMA:
-
Chromomycin A3
- DAPI:
-
4′,6-Diamidino-2-phenlindol
- rDNA:
-
Ribosomal DNA
- TEs:
-
Transposable elements
- SSRs:
-
Microsatellite sequences
- PCR:
-
Polymerase chain reaction
- SSC:
-
Saline–sodium citrate buffer
- SDS:
-
Sodium dodecyl sulfate
- Cy3-dUTP:
-
5-Amino-propargyl-2′-deoxyuridine 5′-triphosphate coupled to red cyanine fluorescent dye
- Alexa:
-
Alexa Fluor 488
- FITC:
-
Fluorescein isothiocyanate
- BSA:
-
Bovine serum albumin
- LINE:
-
Long interspersed nuclear element
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Acknowledgments
We thank Mr. Steve Simmons for proofreading the manuscript.
Funding
The authors received financial support from CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico). Scholarships from CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) were awarded to MA Sader, ZP Costa, and A. Pedrosa-Harand. This study was supported in part by the Coordenacão de Aperfeicoamento de Pessoal de Nível Superior - Brasil (CAPES, Finance Code 001), and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP grant no. 2014/25215-2).
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MAS and APH conceived and designed the research. HB, HP, and MLCV contributed to BAC library, plant material, and bioinformatic data. MAS and YD conducted the experiments. CM, MAS, YD, and ZPC analyzed the data. MAS and APH wrote the manuscript. All authors read and approved the manuscript.
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Figure S1
Fluorescent in situ hybridization to P. edulis chromosomes. (a) BAC Pe134H15 (red); (b) Pe164A12 (red) and 35S rDNA (green); (c) BAC Pe173B16 (green) and Pe164M13 (red); (d) BAC Pe195F4 (red) and 35S rDNA (green); (e) BAC Pe173B16 (red) and 35S rDNA (green). Chromosomes were counterstained with DAPI, are visualized in grey and indicated by number. BACs were labeled with Cy3-dUTP and pseudocolored. Bar = 5 μm. (PNG 8848 kb)
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Sader, M.A., Dias, Y., Costa, Z.P. et al. Identification of passion fruit (Passiflora edulis) chromosomes using BAC-FISH. Chromosome Res 27, 299–311 (2019). https://doi.org/10.1007/s10577-019-09614-0
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DOI: https://doi.org/10.1007/s10577-019-09614-0