Abstract
Purpose
To describe and compare the stromal bed roughness obtained after laser in situ keratomileusis (LASIK) flap creation using a corneal femtosecond laser platform (iFS 150) and a “dual” femtosecond (FS) laser platform (LenSx).
Methods
This ex vivo experimental study was conducted in an animal model using 12 freshly enucleated porcine eyes, six with each femtosecond laser. The standard laser treatment parameters were used for the experiment. After LASIK flap creation, the corneal stromal roughness was assessed using atomic force microscopy (AFM) in contact mode immersed in liquid. In each sample, surface measurements were obtained in 60 regions of six eyes per FS laser in 10 20 × 20-micron areas of the central corneal stroma at 512 × 512-point resolution. The surface roughness was measured and the root-mean-square (RMS) values of the roughness were obtained.
Results
The mean RMS ± standard deviation values were 430 ± 150 nm for the corneal femtosecond laser platform and 370 ± 100 nm for the dual FS laser platform (P < 0.011).
Conclusions
In this experimental study with AFM, we found smoother stromal beds after LASIK flap creation with LenSx compared to iFS 150 kHz. Further studies are needed to understand visual implications of the differences found.
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Funded in part by an unrestricted grant from Abbott Medical Optics (216/3).
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Miguel A. Teus has received research grants form Alcon, Allergan, Abbott, and Novartis; consultant Fees from Alcon, Allergan, Abbott, Novartis, Santen, and Glaukos; and financial support from Alcon, Novartis, and Thea. The other authors declare that they have no conflict of interest.
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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.
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Gros-Otero, J., Ketabi, S., Cañones-Zafra, R. et al. Analysis of corneal stromal roughness after iFS 150 kHz and LenSx femtosecond LASIK flap creation in porcine eyes. Graefes Arch Clin Exp Ophthalmol 257, 2665–2670 (2019). https://doi.org/10.1007/s00417-019-04497-7
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DOI: https://doi.org/10.1007/s00417-019-04497-7