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Characterization and application of a gall midge resistance gene (Gm6) from Oryza sativa ‘Kangwenqingzhan’

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The resistance gene Gm6 was mapped and characterized using near-isogenic and pyramided lines, followed by marker-assisted selection to develop lines with resistance to both gall midge and brown planthopper.

Abstract

The Asian rice gall midge (GM; Orseolia oryzae; Diptera: Cecidomyiidae) is a major destructive pest affecting rice cultivation regions. The characterization of GM-resistance genes and the breeding of resistant varieties are together considered the most efficient strategy for managing this insect. Here, the Gm6 resistance gene derived from the Kangwenqingzhan (KW) variety was found to be located on the long arm of chromosome 4 using the F2 population of 9311/KW. The region was narrowed to a 90-kb segment flanked by the markers YW91 and YW3-4 using backcrossing populations. Based on no-choice feeding and host choice tests, GM development and growth in near-isogenic lines (NILs) were severely restricted compared to that in the 9311 control. On day 8, the average GM body length was 0.69 mm and 0.56 mm on NILs and 9311, respectively, and the differences were more significant at later time points. However, GM insects exhibited no host preference between NILs and 9311, and there was normal egg hatching on the resistant plants. We developed pyramided lines carrying BPH27, BPH36, and Gm6 by crossing and backcrossing with marker-assisted selection. These lines were similar to the KW parent in terms of agronomic traits while also exhibiting high resistance to brown planthopper (BPH) and GM. The present mapping and characterization of Gm6 will facilitate map-based cloning of this important resistance gene and its application in the breeding of insect-resistant rice varieties.

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Acknowledgements

This research was supported by the National Key R&D Program of China (2016YFD0100600), the Guangxi Natural Science Foundation (2018GXNSFAA294028, 2013GXNSFGA019009), and the National Natural Science Foundation of China (31560423).

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  1. Yang Li and Yi Mo contributed equally to this work.

Authors and Affiliations

  1. Agricultural College, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, 530005, China

    Yang Li, Yi Mo, Zhihua Li, Meng Yang, Lihua Tang & Yongfu Qiu

  2. College of Agriculture, Yangtze University, Jingzhou, 434025, China

    Ling Cheng

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Contributions

YQ and YL conceived and designed the study. YL and YM performed most of experiments including the primary and fine mapping, insect characterization test, agronomic survey, and data analysis. ZL, MY, and LT conducted primary mapping and agronomic survey. LC supervised the field tests. YL, YM, and YQ wrote and improved the manuscript. All authors read and approved the manuscript.

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Correspondence to Yongfu Qiu.

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Communicated by Lizhong Xiong.

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Supplementary Figure 1

The pedigree of near isogenic lines (NILs) carrying Gm6 and pyramided lines (PYLs) carrying BPH27, BPH36, and Gm6 developed using marker-assisted selection (MAS) (TIFF 1506 kb)

Supplementary Figure 2

The developed PYL in the KW genetic background and parents. RBPH16 was the donor of BPH27 and BPH36 (Huang et al. 2013, Li et al. 2019) (TIFF 715 kb)

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Li, Y., Mo, Y., Li, Z. et al. Characterization and application of a gall midge resistance gene (Gm6) from Oryza sativa ‘Kangwenqingzhan’. Theor Appl Genet 133, 579–591 (2020). https://doi.org/10.1007/s00122-019-03488-w

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