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RETRACTED ARTICLE: Bifidobacterium plays a protective role in TNF-α-induced inflammatory response in Caco-2 cell through NF-κB and p38MAPK pathways

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This article was retracted on 29 February 2024

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Abstract

Kawasaki disease is an immune-mediated acute, systemic vasculitis and is the leading cause of acquired heart disease in children in the developed world. Bifidobacterium (BIF) is one of the dominant bacteria in the intestines of humans and many mammals and is able to adjust the intestinal flora disorder. The Caco-2 cell monolayers were treated with tumor necrosis factor-α (TNF-α) at 10 ng/ml for 24 h to induce the destruction of intestinal mucosal barrier system. Cells viability was detected through Cell Counting Kit-8 assay. Cell apoptosis was measured by flow cytometry and the expression of apoptosis related proteins was also detected through Western blot. The level of pro-inflammatory cytokines interleukin-6 (IL-6) and IL-8 was detected through ELISA, Western blot and qRT-PCR, respectively. Transepithelial electrical resistance (TEER) assay was conducted to value the barrier function of intestinal mucosa. Cell autophagy and NF-κB and p38MAPK pathways associated proteins were examined through Western blot. In the absence of TNF-α treatment, cell viability and apoptosis showed no significant change. TNF-α decreased cell viability and increased cell apoptosis and BIF treatment mitigated the TNF-α-induced change. Then, we found that BIF treatment effectively suppressed TNF-α-induced overexpression of IL-6 and IL-8. Besides, the results of TEER assay showed that barrier function of intestinal mucosa which was destroyed by TNF-α was effectively recovered by BIF treatment. In addition, TNF-α induced autophagy was also suppressed by BIF. Moreover, TNF-α activated NF-κB and p38MAPK signal pathways were also blocked by BIF, SN50 and SB203580. Our present study reveals that BIF plays a protective role in TNF-α-induced inflammatory response in Caco-2 cells through NF-κB and p38MAPK pathways.

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Acknowledgements

This research received no specific grant from any funding agency in the public, commercial or not-for-profit sectors.

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Authors and Affiliations

  1. Department of Pediatric Cardiology, Nephrology and Rheumatology, The Affiliated Hospital of Qingdao University, Qingdao, 266000, China

    Nana Nie & Cui Bai

  2. Department of Oncology, The Affiliated Hospital of Qingdao University, Qingdao, 266000, China

    Shanai Song

  3. Department of Neonatal Intensive Care Unit, Qingdao Women and Children’s Hospital, No. 6 Tongfu Road, Shibei District, Qingdao, 266034, China

    Yanyan Zhang

  4. Heart Center, Qingdao Women and Children’s Hospital, No. 6 Tongfu Road, Shibei District, Qingdao, 266034, China

    Benzhen Wang & Zipu Li

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  1. Nana Nie
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Correspondence to Zipu Li.

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This article has been retracted. Please see the retraction notice for more detail:https://doi.org/10.1007/s11010-024-04979-1

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Nie, N., Bai, C., Song, S. et al. RETRACTED ARTICLE: Bifidobacterium plays a protective role in TNF-α-induced inflammatory response in Caco-2 cell through NF-κB and p38MAPK pathways. Mol Cell Biochem 464, 83–91 (2020). https://doi.org/10.1007/s11010-019-03651-3

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