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Culture of goat preantral follicles in situ associated with mesenchymal stem cell from bone marrow

Published online by Cambridge University Press:  18 November 2019

Camila Arrivabene Neves ,
Lucilene dos Santos Silva ,
Camila Ernanda Sousa de Carvalho ,
Marina Silva Carvalho ,
José Lindenberg Rocha Sarmento ,
Tânia Vasconcelos Cavalcante ,
Mônica Arrivabene ,
Tábatta Arrivabene Neves ,
Maria Éllida de Sousa Bezerra  and
Antônio Luiz Gomes Júnior
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Camila Arrivabene Neves
Affiliation:
Federal University of Piauí, Integrated Nucleus of Morphology and Stem Cell Research (NUPCelt), Teresina, PI, Brazil
Lucilene dos Santos Silva
Affiliation:
Federal University of Piauí, Integrated Nucleus of Morphology and Stem Cell Research (NUPCelt), Teresina, PI, Brazil
Camila Ernanda Sousa de Carvalho
Affiliation:
Federal University of Piauí, Integrated Nucleus of Morphology and Stem Cell Research (NUPCelt), Teresina, PI, Brazil Federal University of Piauí, Agricultural Science Centre, Teresina, PI, Brazil
Marina Silva Carvalho
Affiliation:
Federal University of Piauí, Integrated Nucleus of Morphology and Stem Cell Research (NUPCelt), Teresina, PI, Brazil
José Lindenberg Rocha Sarmento
Affiliation:
Federal University of Piauí, Agricultural Science Centre, Teresina, PI, Brazil
Tânia Vasconcelos Cavalcante
Affiliation:
Federal University of Piauí, Agricultural Science Centre, Teresina, PI, Brazil
Mônica Arrivabene
Affiliation:
Federal University of Piauí, Agricultural Science Centre, Teresina, PI, Brazil
Tábatta Arrivabene Neves
Affiliation:
Federal University of Piauí, Agricultural Science Centre, Teresina, PI, Brazil
Maria Éllida de Sousa Bezerra
Affiliation:
San Francisco Valley Federal University, Nucleus of Biotechnology Applied to the Development of Ovarian Follicles, Petrolina, PE, Brazil
Antônio Luiz Gomes Júnior
Affiliation:
Federal University of Piauí, Health Science Centre, Teresina, PI, Brazil
Cleidson Manoel Gomes da Silva*
Affiliation:
Federal University of South and Southeast of Pará, Institute of Studies of the Humid Tropics, Xinguara, PA, Brazil
Maria Acelina Martins de Carvalho
Affiliation:
Federal University of Piauí, Integrated Nucleus of Morphology and Stem Cell Research (NUPCelt), Teresina, PI, Brazil
*
Author for correspondence: Cleidson Manoel Gomes da Silva, Federal University of South and Southeast of Pará, Institute of Studies of the Humid Tropics, Street Alberto Santos Dumont, s/n. Xinguara – PA. Brazil. CEP 68557-335. Tel: +55 94 2101 5934. E-mail: gomesvet@hotmail.com
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Summary

This study aims to develop an in vitro co-culture system of in situ goat preantral follicles with bone marrow-derived mesenchymal stem cells (BM-MSC), evaluating the influence of these cells on follicular growth, rate of activation and morphologically normal follicles. Fragments of ovarian cortex were cultured for 1 or 7 days in the presence of BM-MSC (BM-MSC+) and absence of BM-MSC (BM-MSC−). Histological sections of the fragments were analysed and data were obtained regarding morphological classification, survival rate of morphologically normal follicles and rate of follicular activation. Culture medium on days 1 and 7 was also sampled for nitrite concentration and reduced glutathione activity. There was a reduction (P < 0.05) in the percentage of morphologically normal follicles in the BM-MSC+ compared with the fresh control only on the seventh day of culture. When comparing treatments, on the seventh day of culture, a higher rate of morphologically normal preantral follicles was observed in BM-MSC+ (P < 0.05). In both treatments, primordial and developing follicle rates were similar to the fresh control (P > 0.05). When comparing treatments with each other, as well as with the fresh control, no differences were observed in follicular diameter (P > 0.05) or nitrite concentration (P > 0.05). The concentration of reduced glutathione was lower on the seventh day of co-culture in both treatments (P < 0.05). In conclusion, co-culture had no influence on follicular or oocyte development. However, it was critical to maintain the survival of preantral follicles during 7 days of culture.

Keywords

Co-cultureGlutathioneGoatNitriteOvary
Type
Research Article
Information
Zygote , Volume 28 , Issue 1 , February 2020 , pp. 65 - 71
Copyright
© Cambridge University Press 2019 

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