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Effects of bovine serum albumin (BSA) on the excited-state properties of meso-tetrakis(sulfonatophenyl) porphyrin (TPPS4)

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Abstract

To infer changes in the photophysical properties of porphyrins due to complexation with albumin, a combination of Z-scan and conventional spectroscopic techniques was employed. We measured the characteristics of excited states of meso-tetrakis(sulfonatophenyl) porphyrin bound to bovine serum albumin and observed that the binding reduces the intersystem crossing quantum yield and increases the internal conversion one. A reverse saturable absorption process was observed in the nanosecond timescale. These results are important for prediction of the efficiency of this complex in medical and optical applications, because associating porphyrins to proteins enables better accumulation in tumors and improves its stability in optical devices, but at the same time, decreases its triplet quantum yield.

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Acknowledgements

The authors acknowledge the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq Grant nos. 305303/2013-9, 309404/2015-0, 458436/2014-3 and 425124/2018-5), Fundação de Amparo à Pesquisa do Estado de Goiás (FAPEG Grant nos. 201410267001776 and 201710267000533) for this research financial support.

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Authors and Affiliations

  1. Instituto de Física, Universidade Federal de Goiás, Goiânia, GO, 74001-970, Brazil

    Pablo J. Gonçalves, Fabio C. Bezerra, Lais Alonso, Antonio Alonso, Sergio C. Zílio & Iouri E. Borissevitch

  2. Instituto de Química, Universidade Federal de Goiás, Goiânia, GO, 74001-970, Brazil

    Pablo J. Gonçalves

  3. Campus Henrique Santillo, Universidade Estadual de Goiás, Anápolis, GO, 75132-400, Brazil

    Luciane M. Almeida

  4. Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, 74001-970, Brazil

    Guilherme R. L. Souza

  5. Instituto de Física de São Carlos, Universidade de São Paulo, Caixa Postal 369, São Carlos, SP, 13560-970, Brazil

    Sergio C. Zílio

  6. Departamento de Física, FFLCRP, Universidade de São Paulo, Av. Bandeirantes 3900, Ribeirão Preto, SP, 14040-901, Brazil

    Iouri E. Borissevitch

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  1. Pablo J. Gonçalves
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Correspondence to Pablo J. Gonçalves.

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Gonçalves, P.J., Bezerra, F.C., Almeida, L.M. et al. Effects of bovine serum albumin (BSA) on the excited-state properties of meso-tetrakis(sulfonatophenyl) porphyrin (TPPS4) . Eur Biophys J 48, 721–729 (2019). https://doi.org/10.1007/s00249-019-01397-w

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