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Cloning, Expression and Characterization of a Novel α-Amylase from Salinispora arenicola CNP193

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Abstract

α-Amylases are used in various biotechnological processes including the textile, paper, food, biofuels, detergents and pharmaceutical industries. In this study, a novel gene encoding α-amylase was cloned from marine bacterium Salinispora arenicola CNP193 and the protein was expressed in Escherichia coli. The α-amylase gene from S. arenicola CNP193 had a length of 1839 bp and encoded a α-amylase with an estimated molecular mass of 74 kDa. The optimum temperature and pH for the recombinant α-amylase was 50 °C and 7 respectively. Na+, K+ and Ca2+ increased the activity of the recombinant α-amylase whereas the enzyme was inhibited by Cu2+, Zn2+, Hg2+, Pb2+, Fe3+ and Mn2+. Thin layer chromatography results confirmed that monosaccharide, disaccharide and maltotriose are the hydrolysis products. The results of our study suggest that this enzyme has considerable potential in industrial applications.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant No. 31772016), Jiangsu Province Marine Science and Technology Innovation Project (HY2018-10), the Priority Academic Program Development of Jiangsu Higher Education Institutions, the Scientific Research Foundation for the Returned Overseas Chinese Scholars, Six talent peaks project in Jiangsu Provincev (2016-SWYY-195), and Project “333” of Jiangsu Province.

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Authors and Affiliations

  1. Jiangsu Key Laboratory of Marine Bioresource and Environment, Lianyungang, 222005, China

    Shu Liu & Yaowei Fang

  2. University of California San Diego, 9500 Gilman Drive La Jolla, San Diego, CA, 92093, USA

    Sibtain Ahmed

  3. Jiangsu Marine Resources Development Research Institute, Lianyungang, 222000, China

    Shu Liu & Yaowei Fang

  4. College of Marine Life and Fisheries, Huaihai Institute of Technology, Lianyungang, 222005, China

    Yaowei Fang

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  1. Shu Liu
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  2. Sibtain Ahmed
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  3. Yaowei Fang
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Correspondence to Sibtain Ahmed or Yaowei Fang.

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Liu, S., Ahmed, S. & Fang, Y. Cloning, Expression and Characterization of a Novel α-Amylase from Salinispora arenicola CNP193. Protein J 38, 716–722 (2019). https://doi.org/10.1007/s10930-019-09870-3

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