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Quantitative Analysis of the Biologically Active Compounds Present in Leaves of Mexican Sweet Potato Accessions: Phenols, Flavonoids, Anthocyanins, 3,4,5-Tri-Caffeoylquinic Acid and 4-Feruloyl-5-Caffeoylquinic Acid

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Abstract

Sweet potato is one of the oldest crops cultivated in Mexico, and Mesoamerica is considered as a region with the greatest diversity of this species. Therefore, the present study focused on the evaluation of biologically active compounds, such as caffeoylquinic acid derivatives and flavonoid compounds, in sweet potato leaves of 200 accessions of the main producing regions of Mexico. The analysis of total phenol content (TPC) showed a great variability of concentrations among the examined accessions (54.41 to 284.64 mgTPC/g DW). Likewise, total flavonoid content (TFC) was determined and ranged from 10.01 to 40.17 mgTFC /g DW. Finally, total anthocyanin content (TAC) was evaluated and concentrations obtained varied from 0.05 to 0.98 mgTAC/g DW. Additionally, HPLC analysis of all 200 accessions demonstrated the presence of caffeic acid (CA), 5-caffeoylquinic acid (5-CQA), three isomers of di-caffeoylquinic acid (di-CCA) and 4-feruloyl-5-caffeoylquinic acid (4F-5CQA) in all test samples. Only 21 accessions tested showed the quantitative amount of 3,4,5-tri-caffeoylquinic acid (3,4,5-tri-CQA) with concentrations ranging from 44.73 to 193.22 mg/100 g DW and high content of 4F-5CQA (139.46 to 419.99 mg/100 g DW). The gathered data indicate that leaves of Mexican sweet potatoes are a promising source of phenolic compounds with remarkable nutraceutical potential.

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Abbreviations

CA:

Caffeic acid

4,5-Di-CQA:

4,5-di-caffeoylquinic acid

3,4-Di-CQA:

3,4-di-caffeoylquinic acid

3,5-Di-CQA:

3,5-di-caffeoylquinic acid

4F-5CQA:

4-feruloyl-5-caffeoylquinic acid

I. batatas :

Ipomoea batatas

5-CQA:

5-caffeoylquinic acid

3,4,5-Tri-CQA:

3,4,5-tri-caffeoylquinic acid

TAC:

Total anthocyanin content

TFC:

Total flavonoid content

TPC:

Total phenol content

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Acknowledgments

The CONACYT doctoral scholarship of Andrea Torres (scholarship number: 308249); the technical support of Margarita Guzman; project PAPIIT-UNAM-IT202318.

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Authors and Affiliations

  1. Departamento de Alimentos y Biotecnología, Facultad de Química, UNAM, C.P., 04510, Mexico City, Mexico

    Andrea Torres & Arturo Navarro-Ocana

  2. Jardín Botánico, Instituto de Biología, UNAM, C.P., 04510, Mexico City, Mexico

    Francisco Basurto

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  1. Andrea Torres
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Correspondence to Arturo Navarro-Ocana.

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Torres, A., Basurto, F. & Navarro-Ocana, A. Quantitative Analysis of the Biologically Active Compounds Present in Leaves of Mexican Sweet Potato Accessions: Phenols, Flavonoids, Anthocyanins, 3,4,5-Tri-Caffeoylquinic Acid and 4-Feruloyl-5-Caffeoylquinic Acid. Plant Foods Hum Nutr 74, 531–537 (2019). https://doi.org/10.1007/s11130-019-00774-2

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