Abstract
Ractopamine HCl (RHC) is supplemented to feedlot cattle to improve feed efficiency and increase carcass weight. Supplementation of RHC clearly benefits livestock production, but it is of note that the adrenergic system through which it acts is typically associated with stress. The purpose of this study was to identify changes in the transcriptome of whole blood in RHC-supplemented feedlot cattle. We hypothesized that transcripts related to inflammatory processes would be upregulated after 35 days of dietary RHC supplementation. To test this hypothesis, RNA from whole blood collected from 16 cattle before and after supplementation with 300 mg/day of RHC was sequenced using 3′ tag-seq. Eight transcripts were differentially expressed (Adjp < 0.10) between pre- and post-supplementation blood samples. Although several of these transcripts including IFI35, TYROBP, and TP53INP1 are associated with inflammation, a systemic dysregulation of inflammatory pathways was not evident. These data provide insight into the response of cattle to RHC supplementation that will direct future studies examining how the transcriptome of whole blood and other tissues responds during acute exposure to RHC and how this supplement mechanistically improves growth performance.
Data Availability
Transcriptome sequence is available through the Sequence Read Archive at NCBI under BioProject PRJNA543614.
References
Bittner CJ, Greenquist MA, Burken DB, Shreck AL, MacDonald JC, Klopfenstein TJ, Platter WJ, Van Koevering MT, Pyatt NA, Erickson GE (2017) Evaluation of ractopamine hydrochloride (Optaflexx) on growth performance and carcass characteristics of finishing steers across different feeding durations. J Anim Sci 95:485–498
Bouvier-Muller J, Allain C, Tabouret G, Enjalbert F, Portes D, Noirot C, Rupp R, Foucras G (2017) Whole blood transcriptome analysis reveals potential competition in metabolic pathways between negative energy balance and response to inflammatory challenge. Sci Rep 7:2379
Buntyn JO, Burdick Sanchez NC, Schmidt TB, Erickson GE, Sieren SE, Jones SJ, Carroll JA (2016) The metabolic, stress axis, and hematology response of zilpaterol hydrochloride supplemented beef heifers when exposed to a dual corticotropin-releasing hormone and vasopressin challenge. J Anim Sci 94:2798–2810
Cadaret CN, Beede KA, Riley HE, Yates DT (2017) Acute exposure of primary rat soleus muscle to zilpaterol HCl (beta2 adrenergic agonist), TNFalpha, or IL-6 in culture increases glucose oxidation rates independent of the impact on insulin signaling or glucose uptake. Cytokine 96:107–113
Dobin A, Gingeras TR (2015) Mapping RNA-seq reads with STAR. Curr Protoc Bioinformatics 51:11.14.1–11.1419
Finelli MJ, Oliver PL (2017) TLDc proteins: new players in the oxidative stress response and neurological disease. Mamm Genome 28:395–406
Fukuda M, Kanno E, Ishibashi K, Itoh T (2008) Large scale screening for novel Rab effectors reveals unexpected broad Rab binding specificity. Mol Cell Proteomics 7:1031–1042
Hausdorff WP, Caron MG, Lefkowitz RJ (1990) Turning off the signal: desensitization of beta-adrenergic receptor function. FASEB J 4:2881–2889
Kim MH, Yang JY, Upadhaya SD, Lee HJ, Yun CH, Ha JK (2011) The stress of weaning influences serum levels of acute-phase proteins, iron-binding proteins, inflammatory cytokines, cortisol, and leukocyte subsets in Holstein calves. J Vet Sci 12:151–157
Law CW, Chen Y, Shi W, Smyth GK (2014) Voom: precision weights unlock linear model analysis tools for RNA-seq read counts. Genome Biol 15:R29
Luo J, Sladek R, Carrier J, Bader JA, Richard D, Giguere V (2003) Reduced fat mass in mice lacking orphan nuclear receptor estrogen-related receptor alpha. Mol Cell Biol 23:7947–7956
Oster M, Murani E, Ponsuksili S, D’Eath RB, Turner SP, Evans G, Tholking L, Kurt E, Klont R, Foury A, Mormede P, Wimmers K (2014) Transcriptional responses of PBMC in psychosocially stressed animals indicate an alerting of the immune system in female but not in castrated male pigs. BMC Genomics 15:967
Prince OD, Langdon JM, Layman AJ, Prince IC, Sabogal M, Mak HH, Berger AE, Cheadle C, Chrest FJ, Yu Q, Andrews NC, Xue QL, Civin CI, Walston JD, Roy CN (2012) Late stage erythroid precursor production is impaired in mice with chronic inflammation. Haematologica 97:1648–1656
Russell JR, Sexten WJ, Kerley MS, Hansen SL (2016) Relationship between antioxidant capacity, oxidative stress, and feed efficiency in beef steers. J Anim Sci 94:2942–2953
Seillier M, Pouyet L, N’Guessan P, Nollet M, Capo F, Guillaumond F, Peyta L, Dumas JF, Varrault A, Bertrand G, Bonnafous S, Tran A, Meur G, Marchetti P, Ravier MA, Dalle S, Gual P, Muller D, Rutter GA, Servais S, Iovanna JL, Carrier A (2015) Defects in mitophagy promote redox-driven metabolic syndrome in the absence of TP53INP1. EMBO Mol Med 7:802–818
Walker DK, Titgemeyer EC, Baxa TJ, Chung KY, Johnson DE, Laudert SB, Johnson BJ (2010) Effects of ractopamine and sex on serum metabolites and skeletal muscle gene expression in finishing steers and heifers. J Anim Sci 88:1349–1357
Wistow G (2012) The human crystallin gene families. Hum Genomics 6:26
Xiahou Z, Wang X, Shen J, Zhu X, Xu F, Hu R, Guo D, Li H, Tian Y, Liu Y, Liang H (2017) NMI and IFP35 serve as proinflammatory DAMPs during cellular infection and injury. Nat Commun 8:950
Xing J, Xing F, Zhang C, Zhang Y, Wang N, Li Y, Yang L, Jiang C, Zhang C, Wen C, Jiang Y (2014) Genome-wide gene expression profiles in lung tissues of pig breeds differing in resistance to porcine reproductive and respiratory syndrome virus. PLoS One 9:e86101
Yuk JM, Kim TS, Kim SY, Lee HM, Han J, Dufour CR, Kim JK, Jin HS, Yang CS, Park KS, Lee CH, Kim JM, Kweon GR, Choi HS, Vanacker JM, Moore DD, Giguere V, Jo EK (2015) Orphan nuclear receptor ERRalpha controls macrophage metabolic signaling and A20 expression to negatively regulate TLR-induced inflammation. Immunity 43:80–91
Funding
This research was supported by a University of Nebraska-Lincoln Layman award and by the Nebraska Agricultural Experiment Station with funding from the Hatch Multistate Research capacity funding program (Accession Number 1011055) from the United States Department of Agriculture – National Institute of Food and Agriculture.
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All procedures performed in studies involving animals were in accordance with the ethical standards of the Institutional Animal Care and Use Committee at the University of Nebraska – Lincoln, at which the studies were conducted. The University of Nebraska is accredited by AAALAC International.
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Communicated by: Maciej Szydlowski
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Burrack, R.M., Duffy, E.M., Yates, D.T. et al. Whole blood transcriptome analysis in feedlot cattle after 35 days of supplementation with a β1-adrenergic agonist. J Appl Genetics 61, 117–121 (2020). https://doi.org/10.1007/s13353-019-00527-6
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DOI: https://doi.org/10.1007/s13353-019-00527-6