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Influence of prolonged fasting on monoamine oxidase and semicarbazide-sensitive amine oxidase activities in rat white adipose tissue

Influencia del ayuno prolongado sobre la actividad monoamino oxidasa y amino oxidasa sensible al semicarbazide en el tejido adiposo blanco de rata

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Abstract

Monoamine oxidase (MAO) and semicarbazide-sensitive amine oxidase (SSAO) activities are very high in white adipose tissue (WAT). SSAO, also known as Vascular Adhesion Protein-1 in vessels, is present at the surface of fat cells and independent approaches have evidenced its impressive increase during adipogenesis. However, the factors that might regulate the expression SSAO and MAO in adipose tissue are still poorly defined. Here, we report the influence of fasting on MAO and SSAO activities in adipose depots. A decrease of MAO activity occurred after three days of starvation in the intra-abdominal adipose tissue (INWAT) of male Wistar rats, regardless of their initial adiposity or fat loss. The reduced fat stores of seven-week old rats, loosing 59% of INWAT mass during fasting, contained only one half of the MAO activity found in fed control. The same reduction of MAO was observed after prolonged fasting in older rats which lose only 26% of their INWAT during the same starvation duration, leading to a fat mass comparable to that of younger fed control rats. It was therefore the endocrine and metabolic changes occurring during fasting that were responsible for the reduced MAO activity and not the amount of INWAT. Surprisingly, SSAO activity remained unchanged during starvation. In subcutaneous WAT, the changes in MAO and SSAO activities exhibited the same tendencies than those found in INWAT. Taken together, these data show that both MAO and SSAO activities increase in INWAT with age-dependent fattening, and indicate that only MAO diminishes during fasting.

Resumen

La actividad monoamino oxidasa (MAO) y aminooxidasa sensible al semicarbazide (SSAO) están muy elevadas en el tejido adiposo blanco (TAB). SSAO, también conocida como proteína de adhesión vascular-1, está presente en la superficie de los adipocitos maduros. Diferentes investigaciones muestran incremento de su expresión durante la adipogénesis, aunque los factores que regulan la expresión en el TAB no son bien conocidos. Este trabajo describe la influencia del ayuno sobre la actividad MAO y SSAO en TAB. Se ha observado que tras tres días de ayuno disminuye la actividad MAO en el tejido adiposo intra-abdominal (INWAT) de ratas macho Wistar, independientemente de la grasa inicial o de la pérdida de peso inducida por el ayuno. El ayuno redujo un 59% el peso del INWAT y un 50% la actividad MAO en ratas de 7 semanas de edad comparadas con su control (ratas sin ayuno). La misma disminución de la actividad MAO se encontró en ratas de mayor edad (10 semanas) aunque solo perdieron el 26 % de su INWAT durante el mismo ayuno, igualando dicha reserva grasa a la de las ratas más jóvenes sin ayunar. Los resultados indican que serían los cambios endocrinos y metabólicos que ocurren durante el ayuno los responsables de la disminución de la actividad MAO y no la perdida de tejido adiposo en sí. Sorprendentemente, no se observó ningún cambio significativo en la actividad SSAO durante el ayuno. En el tejido adiposo subcutáneo, los cambios de actividad MAO y SSAO mostraron las mismas tendencias que en el INWAT. Los resultados muestran que la edad conlleva un aumento de la actividad de la MAO y de la SSAO en tejido adiposo blanco de rata y que el ayuno reduce la actividad de la MAO, no la de la SSAO.

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Authors and Affiliations

  1. INSERM, U858, I2MR, University Paul Sabatier of Toulouse, France and Semmelweis University of Budapest, Hungary

    Z. Iffiú-Soltész, D. Prévot & C. Carpéné

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  1. Z. Iffiú-Soltész
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  2. D. Prévot
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  3. C. Carpéné
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Correspondence to C. Carpéné.

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Iffiú-Soltész, Z., Prévot, D. & Carpéné, C. Influence of prolonged fasting on monoamine oxidase and semicarbazide-sensitive amine oxidase activities in rat white adipose tissue. J. Physiol. Biochem. 65, 11–23 (2009). https://doi.org/10.1007/BF03165965

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