Inactivation of foot-and-mouth disease virus in epithelium samples for safe transport and processing in low-containment laboratories
Section snippets
Declaration of Competing Interest
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Acknowledgements
This project is supported by Meat & Livestock Australia (MLA), through funding from the Australian Government Department of Agriculture and Water Resources as part of its Rural R&D for Profit program, and by producer levies from Australian FMD-susceptible livestock (cattle, sheep, goats and pigs) industries and Charles Sturt University (CSU), leveraging significant in-kind support from the research partners. The research partners for this project are the Commonwealth Science and Industrial
References (17)
- et al.
Simple, quick and cost-efficient: a universal RT-PCR and sequencing strategy for genomic characterisation of foot-and-mouth disease viruses
J. Virol. Methods
(2017) - et al.
A universal heterologous internal control system for duplex real-time RT-PCR assays used in a detection system for pestiviruses
J. Virol. Methods
(2006) - et al.
Fish viruses stored in RNAlater can remain infectious and even be temporarily protected from inactivation by heat or by tissue homogenates
J. Virol. Methods
(2018) - et al.
Detection and typing of foot-and-mouth disease virus by enzyme-linked immunosorbent assay: a sensitive, rapid and reliable technique for primary diagnosis
Res. Vet. Sci.
(1987) - et al.
Viral infectivity is maintained by an RNA protection buffer
J. Virol. Methods
(2005) - et al.
Iactivation of foot-and-mouth disease virus by pH and temperature changes and by formaldehyde
Proc. Soc. Exp. Biol. Med.
(1957) - et al.
Rescue of foot-and-mouth disease viruses that are pathogenic for cattle from preserved viral RNA samples
PLoS One
(2011) - et al.
Use of a portable real-time reverse transcriptase-polymerase chain reaction assay for rapid detection of foot-and-mouth disease virus
J. Am. Vet. Med. Assoc.
(2002)
Cited by (7)
Evaluation of long-term preservation methods for viral RNA in mosquitoes at room temperature
2024, Journal of Virological MethodsDiagnostic application of formalin fixed archived tissues for detection of foot-and-mouth disease
2023, Journal of Virological MethodsChemical inactivation of foot-and-mouth disease virus in bovine tongue epithelium for safe transport and downstream processing
2022, Journal of Virological MethodsCitation Excerpt :Apart from an apparent reduction in viral RNA in O/ALG/3/2014 samples treated with PAXgene Tissue System Stabiliser at all time points compared to all other samples, there was no drastic effect on RT-qPCR RNA detection following incubation with the different buffers, up to at least 48 h (Fig. 2). Sequencing of the VP1 capsid protein coding region (1D) of FMDV is widely used for determination of serotype, lineage and strain which aids in outbreak characterisation and vaccine strain selection (Dill and Eschbaumer, 2019; Horsington et al., 2020). RNA specimens extracted from the 24-h incubation samples (n = 64) were tested as follows.
Field Evaluation of a Safe, Easy, and Low-Cost Protocol for Shipment of Samples from Suspected Cases of Foot-and-Mouth Disease to Diagnostic Laboratories
2023, Transboundary and Emerging DiseasesDNA/RNA Preservation in Glacial Snow and Ice Samples
2022, Frontiers in MicrobiologyNon-discriminatory Exclusion Testing as a Tool for the Early Detection of Foot-and-Mouth Disease Incursions
2020, Frontiers in Veterinary Science