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Risk assessment of low arsenic exposure using biomarkers of oxidative and genotoxic stress in a piscine model

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Abstract

The high level exposure to arsenic induces marked oxidative and genotoxic stress. However, information on the potential of low level arsenic exposure in this context is still scanty. In the present study, the extent of oxidative stress and genetic toxicity induced by low arsenic exposure was explored in freshwater fish Channa punctatus. Fish were exposed to low levels of arsenic (10 and 50 µg L−1) as well as to its high level (500 µg L−1) using sodium arsenite in aquaria water for 14 consecutive days. The TBARS assay for lipid peroxidation exhibited the increased occurrence of oxidative damage in the erythrocytes of fish at both the lower and higher levels of arsenic exposure. The level of reduced glutathione was also elevated in all the three arsenic exposed groups of fish compared to control. In contrast, significant decline was observed in the levels of three major antioxidant enzymes namely, superoxide dismutase, catalase and glutathione peroxidase, upon exposure to higher as well as lower levels of arsenic. Significant increases in micronucleus induction were found in the erythrocytes of fish even at the low levels of arsenic exposure. The study further revealed the occurrence of DNA fragmentation in the erythrocytes of fish at low arsenic exposures as well. The low level exposure to arsenic (using sodium arsenite), therefore, appeared to be capable of inducing noticeable oxidative stress as well as potential genotoxic effect in Channa punctatus. Moreover, the ability of arsenic to induce oxidative stress invariably appeared correlated with its genotoxic potential.

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Acknowledgements

The financial assistance from DBT-PU-IPLS scheme (Sanction No. BT/PR4577/INF/22/149/2012) of the Department of Biotechnology, Government of India to PKK as Co-Project Investigator, DKJ as Junior Research Fellow and KS as Technical Officer is thankfully acknowledged.

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  1. Department of Zoology, Patna University, Patna, 800 005, India

    Deepak K. Jha, Kumar Sayrav, Gaurav P. Mishra, Anupma Kumari & Parimal K. Khan

  2. Department of Biochemistry, Patna University, Patna, 800 005, India

    Bipin B. Mishra

  3. Kirori Mal College, University of Delhi, New Delhi, 110 007, India

    Amod Kumar

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Jha, D.K., Sayrav, K., Mishra, G.P. et al. Risk assessment of low arsenic exposure using biomarkers of oxidative and genotoxic stress in a piscine model. Ecotoxicology 28, 669–679 (2019). https://doi.org/10.1007/s10646-019-02060-y

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