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MiR-153 regulates cardiomyocyte apoptosis by targeting Nrf2/HO-1 signaling

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Abstract

MicroRNAs (miRNAs) play various roles in the regulation of human disease, including cardiovascular diseases. MiR-153 has been previously shown to be involved in regulating neuron survival during cerebral ischemia/reperfusion (I/R) injury. However, whether miR-153 is involved in I/R-induced cardiomyocyte apoptosis remains to be elucidated. In this study, we aimed to explore the role of miR-153 in the regulation of I/R-induced cardiomyocyte apoptosis and to investigate the miR-153-mediated molecular signaling pathway responsible for its effect on cardiomyocytes using an oxygen-glucose deprivation and reoxygenation (OGD/R) cellular model. We found that OGD/R treatment induced significant upregulation of miR-153 in cardiomyocytes causing reactive oxygen species (ROS) production and cell apoptosis signaling activation and subsequently leading to cardiomyocyte apoptosis. Suppression of miR-153 protected cardiomyocytes against OGD/R treatment. We further identified that nuclear factor-like 2 (Nrf2) is a functional target of miR-153. Nrf2/ heme oxygenase-1 (HO-1) signaling plays a critical role in miR-153 regulated OGD/R-induced cardiomyocyte apoptosis. Our study indicates that the inhibition of miR-153 or restoration of Nrf2 may serve as a potential therapeutic strategy for ischemia/reperfusion injury prevention.

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Abbreviations

I/R:

Ischemia/reperfusion

OGD/R:

Oxygen-glucose deprivation and reoxygenation

Nrf2:

Nuclear factor-like 2

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Author notes

  1. Xianting Zhu and Yuling Zhao contributed equally to this work.

Authors and Affiliations

  1. Department of Nursing, Yidu Central Hospital of Wei Fang, No. 4138, South Linglongshan Road, Weifang, 262500, Shandong, China

    Xianting Zhu & Ling Guo

  2. Department of Emergency, Yidu Central Hospital of Wei Fang, No. 4138, South Linglongshan Road, Weifang, 262500, Shandong, China

    Yuling Zhao & Wei Hou

Authors
  1. Xianting Zhu
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  2. Yuling Zhao
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  3. Wei Hou
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  4. Ling Guo
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Contributions

XTZ and YLZ were contributed equally to this work. Conception and design: XTZ, YLZ, and WH. Collection and assembly of data: YLZ and WH. Data analysis and interpretation: YLZ and WH. Contribution of reagents, materials, and analysis tools wrote the paper: LG. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Ling Guo.

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Research involving human participants and/or animals

This study was approved by the Ethics Committee of the Yidu Central Hospital of Wei Fang.

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Zhu, X., Zhao, Y., Hou, W. et al. MiR-153 regulates cardiomyocyte apoptosis by targeting Nrf2/HO-1 signaling. Chromosome Res 27, 167–178 (2019). https://doi.org/10.1007/s10577-019-09608-y

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  • DOI: https://doi.org/10.1007/s10577-019-09608-y

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