Abstract
Flower development is essential for the sexual reproduction and crop yield of plants. Thus, a better understanding of plant sterility from the perspective of morphological and molecular genetics is imperative. In our previous study, a recessive female-sterile Chinese cabbage mutant fsm was obtained from a doubled haploid line ‘FT’ via an isolated microspore culture combined with EMS mutagenesis. Pistil aniline blue staining and stigma scanning observation showed that the growth of the stigma papillar cells and pollen tubes of the mutant fsm were normal. Therefore, the female sterility was due to abnormal development of the ovules. To map the mutant fsm, 3108 F2 individuals were selected for linkage analysis. Two closely linked markers, Indel-I2 and Indel-I7, were localized on the flanking region of fsm at distances of 0.05 cM and 0.06 cM, respectively. The physical distance between Indel-I2 and Indel-I7 was ~ 1376 kb, with 107 genes remaining in the target region. This region was located on the chromosome A04 centromere, on which low recombination rates and a high frequency of repetitive sequences were present. Whole-genome re-sequencing detected a single-nucleotide (C-to-A) transition (TCG/TAG) on the exon of BraA04001030, resulting in a premature stop codon. Genotyping revealed that the female-sterile phenotype was fully cosegregated with this SNP. BraA04001030 encodes a homologue of STERILE APETALA (SAP) transcriptional regulator, which plays vital roles in floral development. The results of the present study suggest that BraA04001030 is a strong candidate gene for fsm and provide the basis for exploring the molecular mechanism underlying female sterility in Chinese cabbage.
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The research was supported by the National Natural Science Foundation of China (Grant No. 31672144).
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497_2019_368_MOESM1_ESM.tif
Alignment of nucleotide sequences of BraA04001030 between seven recombinant individuals, female-sterile mutant fsm, and wild-type ‘FT’ (TIFF 5158 kb)
497_2019_368_MOESM2_ESM.tif
Phylogenetic tree of SAP homologs. The phylogenetic tree was constructed using the neighbor-joining method of MEGA 5.0 program. The scale bar at bottom represents the genetic distance (TIFF 3223 kb)
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Liu, W., Huang, S., Liu, Z. et al. A missense mutation of STERILE APETALA leads to female sterility in Chinese cabbage (Brassica campestris ssp. pekinensis). Plant Reprod 32, 217–228 (2019). https://doi.org/10.1007/s00497-019-00368-7
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DOI: https://doi.org/10.1007/s00497-019-00368-7