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Anti-inflammatory and antinociceptive effects of an ethanol extract from Senna septemtrionalis

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Abstract

Senna septemtrionalis (Viv.) H.S. Irwin & Barneby (Fabaceae) is a medicinal plant used as a folk remedy for inflammation and pain. The objective of this study was to evaluate the anti-inflammatory and antinociceptive actions of an ethanol extract of Senna septemtrionalis aerial parts (SSE). The in vitro anti-inflammatory effects of SSE were assessed using LPS-stimulated macrophages and the subsequent quantification of the levels of cytokines (IL-6, IL-1β, and TNF-α) with ELISA kits, nitric oxide (NO), and hydrogen peroxide (H2O2). The in vivo anti-inflammatory actions of SSE were evaluated with the TPA-induced ear oedema test and the carrageenan-induced paw oedema test. The antinociceptive actions of SSE (10–200 mg/kg p.o.) were assessed using three models: two chemical assays (formalin-induced orofacial pain and acetic acid-induced visceral pain) and one thermal assay (hot plate). SSE showed in vitro anti-inflammatory actions with IC50 values calculated as follows: 163.3 µg/ml (IL-6), 154.7 µg/ml (H2O2) and > 200 µg/ml (IL-1β, TNF-α, and NO). SSE showed also in vivo anti-inflammatory actions in the TPA test (40% of inhibition of ear oedema) and the carrageenan test (ED50 = 137.8 mg/kg p.o.). SSE induced antinociceptive activity in the formalin orofacial pain test (ED50 = 80.1 mg/kg) and the acetic acid-induced writhing test (ED50 = 110 mg/kg). SSE showed no antinociceptive actions in the hot plate assay. The pre-treatment with glibenclamide abolished the antinociceptive action shown by SSE alone. Overall, SSE exerted in vitro and in vivo anti-inflammatory actions, and in vivo antinociceptive effects by the possible involvement of ATP-sensitive K + channels.

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Acknowledgements

This work was partially supported by the Directorate for Research Support and Postgraduate Programs (DAIP) at University of Guanajuato (DAIP, CIIC 67/2019, provided to AJAC). An experimental section of this work was supported by the program SEP-PRODEP (Apoyo a la Incorporación de Nuevos PTC, Grant Number: UDG-PTC-1438 Number 511-6/18/9169, provided to MAIE).

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Authors and Affiliations

  1. Facultad de Química, Universidad Autónoma de Yucatán, Calle 43, Calle 90 613 x, Inalámbrica, 97069, Mérida, Yucatán, Mexico

    Víctor Ermilo Arana-Argáez, Julio Cesar Lara-Riegos & Emanuel Ceballos-Góngora

  2. Laboratorio de Biotecnología de Productos Naturales, Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Km 4.5 Carretera Federal Atlixco-Metepec, C.P. 74360, Metepec, Puebla, Mexico

    Fabiola Domínguez

  3. Phytochemistry and Healthy Foods Lab., Food Science and Technology Department, CEBAS-CSIC, Campus Universitario Espinardo – 25, 30100, Espinardo, Murcia, Spain

    Diego A. Moreno

  4. Departamento de Clínicas, División de Ciencias Biomédicas, Centro Universitario de los Altos, Universidad de Guadalajara, Av. Rafael Casillas Aceves 1200, 47620, Tepatitlán de Morelos, Jalisco, Mexico

    Mario Alberto Isiordia-Espinoza, Lorenzo Franco-de la Torre & Sergio Sánchez-Enríquez

  5. División de Ciencias Naturales y Exactas, Universidad de Guanajuato, Noria Alta S/N, C.P. 36050, Guanajuato, Gto, Mexico

    Juan Ramón Zapata-Morales & Angel Josabad Alonso-Castro

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  1. Víctor Ermilo Arana-Argáez
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  2. Fabiola Domínguez
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  3. Diego A. Moreno
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  4. Mario Alberto Isiordia-Espinoza
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  5. Julio Cesar Lara-Riegos
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  6. Emanuel Ceballos-Góngora
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  7. Juan Ramón Zapata-Morales
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  8. Lorenzo Franco-de la Torre
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  9. Sergio Sánchez-Enríquez
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  10. Angel Josabad Alonso-Castro
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Correspondence to Angel Josabad Alonso-Castro.

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Arana-Argáez, V.E., Domínguez, F., Moreno, D.A. et al. Anti-inflammatory and antinociceptive effects of an ethanol extract from Senna septemtrionalis. Inflammopharmacol 28, 541–549 (2020). https://doi.org/10.1007/s10787-019-00657-7

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