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Fusarium oxysporum KB-3 from Bletilla striata: an orchid mycorrhizal fungus

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Abstract

Orchid mycorrhizal fungi are essential for the seed germination and vegetative growth of orchids. The orchid Bletilla striata has great medical value in China because its tuber is rich in mannan. Some endophytic fungi were isolated from the roots of B. striata. The isolate KB-3 was selected for experiments because it could promote the germination of B. striata seeds. Based on morphological characters and phylogenetic analysis, the isolate KB-3 was identified as Fusarium oxysporum. Co-cultivation experiments of KB-3 with B. striata and Dendrobium candidum were performed to demonstrate orchid mycorrhizal structures. Microscopic examination showed that KB-3 established colonization and produced coiled hyphal structures known as pelotons within the cortical cells of both orchid roots. The results confirm that F. oxysporum KB-3 can behave as an orchid mycorrhizal fungus.

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Funding

This study is funded by the National Key Research and Development Program (2017YFD0201106), the Natural Science Fund of Education Department of Hubei province (D20181304), and the vegetable fertilizer and pesticide reducing team project of Hubei province.

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Author notes

  1. Ke Zhang

    Present address: Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences, Allmas alle 5, 75651, Uppsala, Sweden

  2. Jianwei Jiang and Ke Zhang contributed equally to this work.

Authors and Affiliations

  1. College of Agriculture, Yangtze University, Jingzhou, 434025, Hubei, China

    Jianwei Jiang, Ke Zhang, Sheng Cheng, Qianwen Nie, Qingqing Chen, Jinglong Zhou, Xiao Zhen, Xue ting Li, Tong wen Zhen, Mingyue Xu, Zhengxiang Sun & Yi Zhou

  2. Qingdao Agricultural University, Qingdao, 266109, Shandong, China

    Shen-xian Zhou

  3. Environmental Sciences, University of Guelph, Guelph, Ontario, Canada

    Tom Hsiang

Authors
  1. Jianwei Jiang
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  2. Ke Zhang
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  3. Sheng Cheng
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Contributions

JJ executed all experiments except phylogenetic analysis and wrote the draft, KZ did the phylogenetic analysis and manuscript writing, ZS and YZ designed the experiments.

Corresponding authors

Correspondence to Zhengxiang Sun or Yi Zhou.

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Jiang, J., Zhang, K., Cheng, S. et al. Fusarium oxysporum KB-3 from Bletilla striata: an orchid mycorrhizal fungus. Mycorrhiza 29, 531–540 (2019). https://doi.org/10.1007/s00572-019-00904-3

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