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The NADPH oxidase in Volvariella volvacea and its differential expression in response to mycelial ageing and mechanical injury

  • Bacterial, Fungal and Virus Molecular Biology - Research Paper
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Abstract

NADPH oxidases are enzymes that have been reported to generate reactive oxygen species (ROS) in animals, plants and many multicellular fungi in response to environmental stresses. Six genes of the NADPH oxidase complex components, including vvnoxa, vvnoxb, vvnoxr, vvbema, vvrac1 and vvcdc24, were identified based on the complete genomic sequence of the edible fungus Volvariella volvacea. The number of vvnoxa, vvrac1, vvbema and vvcdc24 transcripts fluctuated with ageing, and the gene expression patterns of vvnoxa, vvrac1 and vvbema were significantly positively correlated. However, the expression of vvnoxb and vvnoxr showed no significant difference during ageing. In hyphae subjected to mechanical injury stress, both O2− and H2O2 concentrations were increased. The expression of vvnoxa, vvrac1, vvbema and vvcdc24 was substantially upregulated, but vvnoxb and vvnoxr showed no response to mechanical injury stress at the transcriptional level. Additionally, the transcription of vvnoxa, vvrac1, vvbema and vvcdc24 could be repressed when the intracellular ROS were eliminated by diphenyleneiodonium (DPI) chloride and reduced glutathione (GSH) treatments. These results indicated a positive feedback loop involving NADPH oxidase and intracellular ROS, which might be the reason for the oxidative burst during injury stress.

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Acknowledgements

The authors thank the “Excellent initiative” for graduate students of College of Life Sciences, Fujian Agriculture and Forestry University; the Fujian Edible Fungi Engineering Technology Research Center and the National Fungi Breeding Center (Fujian Branch) provide the experimental facilities for this study.

Funding

This work was financially supported by grants from the China Agriculture Research System (CARS24), the National Key Basic Research Program of China (2014CB138302) and the Scientific Research Foundation of Graduate School of Fujian Agriculture and Forestry University.

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Authors and Affiliations

  1. Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China

    Jun-Jie Yan, Zong-Jun Tong, Yuan-Yuan Liu, Zi-Yan Lin, Ying Long, Xing Han, Wei-Nan Xu, Qian-Hui Huang, Yong-Xin Tao & Bao-Gui Xie

  2. College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

    Xing Han, Wei-Nan Xu, Qian-Hui Huang & Yong-Xin Tao

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  1. Jun-Jie Yan
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Contributions

XBG and YJJ conceived and designed the experiments; YJJ, TZJ, LYY, LZY, LY, HX and XWN performed the experiments; YJJ, HQH and TYX analysed the data; YJJ, LYY and TZJ contributed reagents/materials/analysis tools; YJJ wrote the paper; XBG edited the paper; and all authors reviewed the manuscript.

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Correspondence to Bao-Gui Xie.

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Yan, JJ., Tong, ZJ., Liu, YY. et al. The NADPH oxidase in Volvariella volvacea and its differential expression in response to mycelial ageing and mechanical injury. Braz J Microbiol 51, 87–94 (2020). https://doi.org/10.1007/s42770-019-00165-4

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