Skip to main content
SpringerLink
Log in

Characterisation of Two Polyketides from Streptomyces sp. SKH1-2 Isolated from Roots of Musa (ABB) cv. ‘Kluai Sao Kratuep Ho’

  • Original Article
  • Published:
International Microbiology Aims and scope Submit manuscript

Abstract

An endophytic actinomycete strain SKH1-2 isolated from Musa (ABB) cv. ‘Kluai Sao Kratuep Ho’ collected in Suphan Buri province (14° 54′ 22.5″ N/100° 04′ 50″ E), Thailand, was identified as Streptomyces pseudovenezuelae based on phenotypic and chemotaxonomic characteristics, and 16S rRNA sequence analyses. A chemical investigation led to the isolation of two polyketide molecules from the n-butanol crude extract of the strain SKH1-2 culture broth. The compounds were purified using various chromatographic techniques and identified using spectroscopic methods compared with earlier published data. Compound 1, chartreusin, is known as an anti-Gram (+) bacterial compound and was active against Bacillus subtilis ATCC 6633, Kocuria rhizophila ATCC 9341 and Staphylococcus aureus ATCC 6538p with MIC values of 3.1, 1.6 and 12.5 μg/mL, respectively. Compound 2, lumichrome, did not show activity against all tested microbes. To our knowledge, this is the first report of chartreusin and lumichrome isolated from S. pseudovenezuelae. Taken together, it could be proved that Thai plant species are valuable reservoirs of interesting endophytic actinomycetes producing several interesting biologically active compounds.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Aoyama Y, Katayama T, Yamamoto M, Tanaka H, Kon K (1992) A new antitumor antibiotic product, demethylchartreusin. Isolation and biological activities. J Antibiot 45:875–878

    Article  CAS  Google Scholar 

  • Arai T (1975) Culture media for actinomycetes. The Society for Actinomycetes, Japan

    Google Scholar 

  • Berrocal MM, Rodríguez J, Ball AS, Pérez-Leblic MI, Arias ME (1997) Solubilisation and mineralisation of [14C] lignocellulose from wheat straw by Streptomyces cyaneus CECT 3335 during growth in solid-state fermentation. Appl Microbiol Biotechnol 48:379–384

    Article  CAS  Google Scholar 

  • Clinical and Laboratory Standards Institute (CLSI) (2012) Method for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard. M07-A9, Volume 32, No. 3, Replaces M07-A8, Volume 29, No .2

  • Ding L, Maier A, Fiebig HH, Lin WH, Peschel G, Hertweck C (2012) Kandenols A-E, eudesmenes from an endophytic Streptomyces sp of the mangrove tree Kandelia candel. J Nat Prod 75:2223–2227

    Article  CAS  Google Scholar 

  • Ding L, Goerls H, Dornblut K, Lin WH, Maier A, Fiebig HH, Hertweck C (2015) Bacaryolanes A-C, rare bacterial caryolanes from a mangrove endophyte. J Nat Prod 78:2963–2967

    Article  CAS  Google Scholar 

  • El-Shatoury SA, El-Kraly OA, Trujillo ME, El-Kazzaz WM, El-Din ESG, Dewedar A (2013) Generic and functional diversity in endophytic actinomycetes from wild Compositae plant species at South Sinai - Egypt. Res Microbiol 164:761–769

    Article  Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Gordon RE, Barnett DA, Handerhan JE, Hor-Nay Pang C (1974) Nocardia coeliaca, Nocardia autotrophica, and nocardin strain. Int J Syst Bacteriol 24:54–63

    Article  Google Scholar 

  • Inahashi Y et al (2011) Spoxazomicins A-C, novel antitrypanosomal alkaloids produced by an endophytic actinomycete, Streptosporangium oxazolinicum K07-0460T. J Antibiot 64:303–307

    Article  CAS  Google Scholar 

  • Inahashi Y et al (2015) Actinoallolides A-E, new anti-trypanosomal macrolides, produced by an endophytic actinomycete, Actinoallomurus fulvus MK10-036. Org Lett 17:864–867

    Article  CAS  Google Scholar 

  • Kelly KL (1964) Inter-Society Colour Council - National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. US Government Printing Office, Washington DC

    Google Scholar 

  • Kimura MA (1980) Simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    Article  CAS  Google Scholar 

  • Kudo T, Matsushima K, Itoh T, Sasaki J, Suzuki K (1998) Description of four new species of the genus Kineosporia: Kineosporia succinea sp. nov., Kineosporia rhizophila sp. nov., Kineosporia mikuniensis sp. nov. and Kineosporia rhamnosa sp. nov., isolated from plant samples, and amended description of the genus Kineosporia. Int J Syst Bacteriol 48 Pt 4:1245–1255

    Article  CAS  Google Scholar 

  • Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874

    Article  CAS  Google Scholar 

  • Kuncharoen N, Pittayakhajonwut P, Tanasupawat S (2018) Micromonospora globbae sp nov., an endophytic actinomycete isolated from roots of Globba winitii C. H. Wright. Int J Syst Evol Microbiol 68:1073–1077

    Article  CAS  Google Scholar 

  • Küster E, Williams ST (1964) Selection of media for isolation of streptomycetes. Nature 202:928–929

    Article  Google Scholar 

  • Lane DJ (1991) 16S/23S rRNA sequencing. Nucleic acid techniques in bacterial systematics. Wiley, Chichester

    Google Scholar 

  • Leach BE, Calhoun KM, Johnson LE, Teeters TM, Jackson WG (1953) Chartreusin, a new antibiotic produced by Streptomyces chartreusis, a new species. J Am Chem Soc 75:4011–4012

    Article  CAS  Google Scholar 

  • Lechevalier HA, Lechevalier MP, Gerber NN (1971) Chemical composition as a criterion in the classification of actinomycetes. Adv Appl Microbiol 14:47–72

    Article  CAS  Google Scholar 

  • Liu C, Cao Z, Zhang W, Tickner J, Qiu H, Wang C, Chen K, Wang Z, Tan R, Dong S, Xu J (2018) Lumichrome inhibits osteoclastogenesis and bone resorption through suppressing RANKL-induced NFAT activation and calcium signaling. J Cell Physiol 233:8971–8983

    Article  CAS  Google Scholar 

  • Makoto H, Hidetoshi E, Masami T, Takeshi H, Hiroshi N, Tsuyoshi T, Hamao U (1982) An inhibitor of sodium-potassium ATPase produced by Streptomyces pseudovenezuelae MF722-02; purification and properties. J Antibiot 35:1448–1453

    Article  Google Scholar 

  • Mearns-Spragg A, Bregu M, Boyd KG, Burgess JG (1998) Cross-species induction and enhancement of antimicrobial activity produced by epibiotic bacteria from marine algae and invertebrates, after exposure to terrestrial bacteria. Lett Appl Microbiol 27:142–146

    Article  CAS  Google Scholar 

  • Omura S, Tanaka H, Iwai Y, Nishigaki K, Awaya J, Takahashi Y, Masuma R (1978) A new antibiotic, setomimycin, produced by a strain of Streptomyces. J Antibiot 31:1091–1098

    Article  CAS  Google Scholar 

  • Petinate SD, Branquinha MH, Coelho RR, Vermelho AB, Giovanni-De-Simone S (1999) Purification and partial characterization of an extracellular serine-proteinase of Streptomyces cyaneus isolated from Brazilian cerrado soil. J Appl Microbiol 87:557–563

    Article  CAS  Google Scholar 

  • Pholo M, Coetzee B, Maree HJ, Young PR, Lloyd JR, Kossmann J, Hills PN (2018) Cell division and turgor mediate enhanced plant growth in Arabidopsis plants treated with the bacterial signalling molecule lumichrome. Planta 248:477–488

    Article  CAS  Google Scholar 

  • Pridham TG (1970) New names and new combinations in the order Actinomycetales Buchanan 1917. US Dept Agric Tech Bull 1424:1–55

    Google Scholar 

  • Qin S, Li J, Chen HH, Zhao GZ, Zhu WY, Jiang CL, Xu LH, Li WJ (2009) Isolation, diversity, and antimicrobial activity of rare actinobacteria from medicinal plants of tropical rain forests in Xishuangbanna, China. Appl Environ Microbiol 75:6176–6186

    Article  CAS  Google Scholar 

  • Rivlin RS (1973) Riboflavin and cancer: a review. Cancer Res 33:1977–1986

    CAS  PubMed  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  Google Scholar 

  • Shi YS, Zhang Y, Chen XZ, Zhang N, Liu YB (2015) Metabolites produced by the endophytic fungus Aspergillus fumigatus from the stem of Erythrophloeum fordii Oliv. Molecules 20:10793–10799

    Article  CAS  Google Scholar 

  • Shirling EB, Gottlieb D (1966) Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340

    Article  Google Scholar 

  • Stackebrandt E, Ebers J (2006) Taxonomic parameters revisited: tarnished gold standards. Microbiology Today 33:152–155

    Google Scholar 

  • Staneck JL, Roberts GD (1974) Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231

    CAS  PubMed  PubMed Central  Google Scholar 

  • Strobel G, Daisy B, Castillo U, Harper J (2004) Natural products from endophytic microorganisms. J Nat Prod 67:257–268

    Article  CAS  Google Scholar 

  • Supong K et al (2016) Antimicrobial compounds from endophytic Streptomyces sp BCC72023 isolated from rice (Oryza sativa L.). Res Microbiol 167:290–298

    Article  CAS  Google Scholar 

  • Suriyachadkun C, Chunhametha S, Thawai C, Tamura T, Potacharoen W, Kirtikara K, Sanglier JJ (2009) Planotetraspora thailandica sp. nov., isolated from soil in Thailand. Int J Syst Evol Microbiol 59:992–997

    Article  CAS  Google Scholar 

  • Tsukamoto S, Kato H, Hirota H, Fusetani N (1999) Lumichrome - a larval metamorphosis-inducing substance in the ascidian Halocynthia roretzi. Eur J Biochem 264:785–789

    Article  CAS  Google Scholar 

  • Yamamoto K, Asano Y (2015) Efficient production of lumichrome by Microbacterium sp. strain TPU 3598. Appl Environ Microbiol 81:7360–7367

    Article  CAS  Google Scholar 

  • Yoon SH, Ha SM, Kwon S, Lim J, Kim Y, Seo H, Chun J (2017) Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 67:1613–1617

    Article  CAS  Google Scholar 

  • Yu Z et al (2011) Bafilomycins produced by an endophytic actinomycete Streptomyces sp. YIM56209. J Antibiot 64:159–162

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We faithfully thank Dr. A. Matsumoto, Dr. K. Nonaka, Dr. Y. Asami and Mr. M. Watanabe, Department of Drug Discovery, Kitasato University, for helpful the antimicrobial activity assay.

Funding

We thank the 2015 Royal Golden Jubilee Ph. D. Programme as a scholarship to N. K. for financial support under the Thailand Research Fund.

Author information

Authors and Affiliations

  1. Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, 10330, Thailand

    Nattakorn Kuncharoen & Somboon Tanasupawat

  2. Graduate School of Infection Control Sciences, Kitasato University, Minato-ku, Tokyo, 108-8641, Japan

    Wataru Fukasawa, Masato Iwatsuki, Mihoko Mori & Kazuro Shiomi

  3. Kitasato Institute for Life Sciences, Kitasato University, Minato-ku, Tokyo, 108-8641, Japan

    Wataru Fukasawa, Masato Iwatsuki, Mihoko Mori & Kazuro Shiomi

Authors
  1. Nattakorn Kuncharoen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wataru Fukasawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Masato Iwatsuki
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mihoko Mori
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Kazuro Shiomi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Somboon Tanasupawat
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Somboon Tanasupawat.

Ethics declarations

Conflict of Interest

The authors declare that they have no conflict of interest.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kuncharoen, N., Fukasawa, W., Iwatsuki, M. et al. Characterisation of Two Polyketides from Streptomyces sp. SKH1-2 Isolated from Roots of Musa (ABB) cv. ‘Kluai Sao Kratuep Ho’. Int Microbiol 22, 451–459 (2019). https://doi.org/10.1007/s10123-019-00071-7

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10123-019-00071-7

Keywords

Search

Navigation