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A proof of principle experiment for microbeam radiation therapy at the Munich compact light source

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Abstract

Microbeam radiation therapy (MRT), a preclinical form of spatially fractionated radiotherapy, uses an array of microbeams of hard synchrotron X-ray radiation. Recently, compact synchrotron X-ray sources got more attention as they provide essential prerequisites for the translation of MRT into clinics while overcoming the limited access to synchrotron facilities. At the Munich compact light source (MuCLS), one of these novel compact X-ray facilities, a proof of principle experiment was conducted applying MRT to a xenograft tumor mouse model. First, subcutaneous tumors derived from the established squamous carcinoma cell line FaDu were irradiated at a conventional X-ray tube using broadbeam geometry to determine a suitable dose range for the tumor growth delay. For irradiations at the MuCLS, FaDu tumors were irradiated with broadbeam and microbeam irradiation at integral doses of either 3 Gy or 5 Gy and tumor growth delay was measured. Microbeams had a width of 50 µm and a center-to-center distance of 350 µm with peak doses of either 21 Gy or 35 Gy. A dose rate of up to 5 Gy/min was delivered to the tumor. Both doses and modalities delayed the tumor growth compared to a sham-irradiated tumor. The irradiated area and microbeam pattern were verified by staining of the DNA double-strand break marker γH2AX. This study demonstrates for the first time that MRT can be successfully performed in vivo at compact inverse Compton sources.

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Acknowledgements

This work has been supported by the Deutsche Forschungsgemeinschaft (MAP C.3.4, CALA) Cluster of Excellence: Munich-Centre for Advanced Photonics as well as the Munich School of BioEngineering of the Technical University of Munich. This work was also supported by the Centre of Advanced Laser Applications with respect to resources necessary for and at the Munich Compact Light Source.

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Authors and Affiliations

  1. Institute of Radiation Medicine, Helmholtz Zentrum München GmbH, 85764, Neuherberg, Germany

    Annique C. Dombrowsky, Marlon Stein, Stefan Bartzsch, Stephanie E. Combs & Thomas E. Schmid

  2. Department of Radiation Oncology, School of Medicine, Klinikum rechts der Isar, Technical University of Munich, 81675, Munich, Germany

    Annique C. Dombrowsky, Karin Burger, Ann-Kristin Porth, Marlon Stein, Stefan Bartzsch, Stephanie E. Combs, Jan J. Wilkens & Thomas E. Schmid

  3. Chair of Biomedical Physics, Department of Physics, Technical University of Munich, 85748, Garching, Germany

    Karin Burger, Martin Dierolf, Klaus Achterhold, Franz Pfeiffer & Jan J. Wilkens

  4. Munich School of BioEngineering, Technical University of Munich, 85748, Garching, Germany

    Karin Burger, Martin Dierolf, Benedikt Günther, Klaus Achterhold, Bernhard Gleich & Franz Pfeiffer

  5. Research Unit Analytical Pathology, Helmholtz Zentrum München GmbH, 85764, Neuherberg, Germany

    Annette Feuchtinger

  6. Helmholtz-Zentrum Dresden-Rossendorf, 01328, Dresden, Germany

    Elke Beyreuther

  7. OncoRay, National Center for Radiation Research in Oncology, Faculty of Medicine, University Hospital Carl Gustav Carus, Technische Universität Dresden, Helmholtz-Zentrum Dresden-Rossendorf, 01328, Dresden, Germany

    Elke Beyreuther

  8. German Consortium for Translational Cancer Research, Deutsches Konsortium für Translationale Krebsforschung (dktk), Technical University Munich, 81675, Munich, Germany

    Stephanie E. Combs

  9. Department of Diagnostic and Interventional Radiobiology, School of Medicine, Klinikum rechts der Isar, Technical University of Munich, 81675, Munich, Germany

    Franz Pfeiffer

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  1. Annique C. Dombrowsky
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  2. Karin Burger
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Correspondence to Thomas E. Schmid.

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All applicable national and institutional guidelines for the care and use of animals were followed. All procedures performed in this study involving animals were in accordance with ethical standards of the institution at which the study was conducted (project license 55.2-1-54-2531-62-2016).

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Dombrowsky, A.C., Burger, K., Porth, AK. et al. A proof of principle experiment for microbeam radiation therapy at the Munich compact light source. Radiat Environ Biophys 59, 111–120 (2020). https://doi.org/10.1007/s00411-019-00816-y

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