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Adventitious root cultures of Oplopanax elatus inhibit LPS-induced inflammation via suppressing MAPK and NF-κB signaling pathways

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Abstract

Bioreactor-cultured adventitious roots (ARs) of the endangered medicinal plant Oplopanax elatus Nakai is a novel alternative plant material. To utilize ARs in the product production, the present study investigated the anti-inflammatory effect of O. elatus ARs. In the in vivo experiment, lipopolysaccharide (LPS)-induced acute lung injury disease model was established and several inflammatory indexes were determined. For the LPS-stimulated mice, after pretreatment of AR crude extract (200 mg/kg), cell infiltration in lungs was decreased, the production of proinflammatory mediators, including nitric oxide (NO), tumor necrosis factor (TNF)-α, and interleukin (IL)-6, and 1β in the bronchoalveolar lavage fluid was evidently reduced, which indicated that O. elatus ARs had an anti-inflammatory effect. In the in vitro experiment, ethyl acetate (EtOAc) fractions (12.5, 25, and 50 μg/mL) were used to treat LPS-induced peritoneal macrophages (PMs) of mice. The production of NO, prostaglandin E2, TNF-α, IL-6, and IL-1β in LPS-stimulated PMs was obviously inhibited (p < 0.05) after pretreatment with EtOAc fractions, and the expression of the inducible nitric oxide synthase and cyclooxygenase were also suppressed. To clarify the anti-inflammatory mechanism, effects of EtOAc fraction on changes of proteins related to the pathways of mitogen-activated protein kinases (MAPKs) and nuclear factor-kappa B (NF-κB) were investigated. The phosphorylation of extracellular regulated protein kinases, c-jun n-terminal kinase, and p38 MAPK in LPS-induced PMs was inhibited after pretreatment of EtOAc fractions. In addition, EtOAc fractions enhanced inhibitor of nuclear factor-kappa B-α expression and decreased nuclear translocation of p65 NF-κB. Thus, EtOAc from O. elatus ARs is involved in regulating MAKP and NF-κB signaling pathways to inhibit LPS-induced inflammation.

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Funding

This work was supported by the Jilin Scientific and Technological Development Program (Grant No. 20180101278JC) and the National Natural Science Foundation of China (Grant No. 21662038).

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Authors and Affiliations

  1. Key Laboratory of Natural Resource of Changbai Mountain and Functional Molecules, Ministry of Education, Yanbian University, Yanji, 133002, Jilin, China

    Wen Tian, Cheng-Ri Yin, Xiao-Long Jiang, Hao-Ding Sun, Xiao-Li An, Jun Jiang & Mei-Lan Lian

  2. Department of Urology, College of Medicine, Chungbuk National University, Cheongju, 28644, Republic of Korea

    Xuan-Mei Piao

Authors
  1. Wen Tian
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  2. Xuan-Mei Piao
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  3. Cheng-Ri Yin
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  6. Xiao-Li An
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  7. Jun Jiang
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  8. Mei-Lan Lian
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Correspondence to Jun Jiang or Mei-Lan Lian.

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Editor: Tetsuji Okamoto

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Tian, W., Piao, XM., Yin, CR. et al. Adventitious root cultures of Oplopanax elatus inhibit LPS-induced inflammation via suppressing MAPK and NF-κB signaling pathways. In Vitro Cell.Dev.Biol.-Animal 55, 766–775 (2019). https://doi.org/10.1007/s11626-019-00396-7

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