Issue 1, 2010
From the journal:

Molecular BioSystems

Temperature and salt concentration alter base-sequence selectivity of a duplex DNA-bindingprotein†

Satoru Nagatoishi,a   Yoshikazu Tanaka,a   Motonori Kudoua  and  Kouhei Tsumoto*a  

* Corresponding authors

a Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan
E-mail: tsumoto@k.u-tokyo.ac.jp
Fax: +81-4-7136-3601
Tel: +81-4-7136-5402

Abstract

A structural polymorphism of nucleic acids, which depends on the concentration of cations and the conditions of hydration, are strongly involved with interactions between DNA and proteins. In this paper, we report that different DNA sequences bound to hyperthermostable TATA-box-binding protein (PhoTBP) at different combinations of temperature and salt concentration in in vitro selection experiments. As a result of the interaction of-these selected DNAs with PhoTBP, characteristic changes in the numbers of water molecules and ions occurred under each condition of the selection experiment. This finding could help us to understand the solvent environment-dependent preference for base sequences in protein–DNA interactions.

Graphical abstract: Temperature and salt concentration alter base-sequence selectivity of a duplex DNA-bindingprotein

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Article information

DOI
https://doi.org/10.1039/B914828K
Article type
Communication
Submitted
22 Jul 2009
Accepted
05 Oct 2009
First published
19 Oct 2009

Mol. BioSyst., 2010,6, 98-101

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Temperature and salt concentration alter base-sequence selectivity of a duplex DNA-bindingprotein

S. Nagatoishi, Y. Tanaka, M. Kudou and K. Tsumoto, Mol. BioSyst., 2010, 6, 98 DOI: 10.1039/B914828K

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