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RETRACTED ARTICLE: Inhibition of microRNA-875-5p promotes radioiodine uptake in poorly differentiated thyroid carcinoma cells by upregulating sodium–iodide symporter

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This article was retracted on 14 July 2022

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Abstract

Background and Aim

Poorly differentiated thyroid carcinoma (PDTC) is an endocrine malignancy that is challenging to treat due to its limited radioiodine uptake. microRNAs (miRNAs or miRs) have been shown to be useful in treating many types of tumors, including PDTC. This study aims to evaluate the potential effect of miR-875-5p on the radioiodine uptake of PDTC and to clarify the underlying mechanisms.

Methods

Expression of miR-875-5p and sodium–iodide symporter (NIS) in tissues and cell lines was determined using RT-qPCR. The binding relationship between miR-875-5p and NIS was predicted through in silico analysis and verified by dual-luciferase reporter gene assay. A series of miR-875-5p mimic, miR-875-5p inhibitor, shRNA against NIS, and overexpressed NIS plasmids were introduced into PDTC cells. We then evaluated the cell viability, colony formation, apoptosis, and radioiodine uptake of each PDTC sample via CCK-8 assay, clonogenic assay, flow cytometry, and γ counter, respectively.

Results

miR-875-5p was found to be highly expressed, but NIS was poorly expressed in DTC tissues and PDTC cell lines. NIS was verified to be a target gene of miR-875-5p. Upregulation of miR-875-5p was found to induce PDTC cell proliferation, and reduce apoptosis and radioiodine uptake in vitro through down-regulation of NIS. In an in vivo orthotopic model, the enhancement of miR-875-5p led to the reduction of NIS expression and radioiodine uptake in the thyroid tumors.

Conclusions

Altogether, the findings of the current study suggest that down-regulated miR-875-5p expression could promote its target gene NIS to increase radioiodine uptake in PDTC, constituting a preventive strategy against PDTC.

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Acknowledgements

We would like to acknowledge the reviewers for their helpful comments on this paper.

Funding

This work was supported by the National Natural Science Foundation of China (No. 81070637, No. 81579742).

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Authors and Affiliations

  1. Department of Endocrinology, Shandong Provincial Qianfoshan Hospital, Shandong University, No. 16766, Jingshi Road, Jinan, 250014, Shandong Province, People’s Republic of China

    Y. Tang, H. Shang & L. Liao

  2. Department of Endocrinology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, 264000, People’s Republic of China

    Y. Tang, X. Meng, X. Yu & S. Chen

  3. Department of Endocrinology, Qilu Hospital of Shandong University, No. 107, Wenhua West Road, Jinan, 250012, Shandong Province, People’s Republic of China

    J. Dong

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  1. Y. Tang
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Correspondence to L. Liao or J. Dong.

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Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The study was conducted in accordance with the Declaration of Helsinki. The experiments that involved human subjects were approved by the Ethics Committee of the Affiliated Yantai Yuhuangding Hospital of Qingdao University. Experimental procedures performed on animals used in medical research were conducted under the approval of the Animal Ethics Committee of the Affiliated Yantai Yuhuangding Hospital of Qingdao University.

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Participating patients have signed informed consents prior to the study.

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This article has been retracted. Please see the retraction notice for more detail: https://doi.org/10.1007/s40618-022-01852-0"

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Tang, Y., Meng, X., Yu, X. et al. RETRACTED ARTICLE: Inhibition of microRNA-875-5p promotes radioiodine uptake in poorly differentiated thyroid carcinoma cells by upregulating sodium–iodide symporter. J Endocrinol Invest 43, 439–450 (2020). https://doi.org/10.1007/s40618-019-01125-3

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  • DOI: https://doi.org/10.1007/s40618-019-01125-3

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