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Bioprocess optimization and production of biosurfactant from an unexplored substrate: Parthenium hysterophorus

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Abstract

Biosurfactants are one among the best alternative for synthetic surfactants that are exploited by many researchers. Several agro wastes help to reduce the cost biosurfactants by being renewable and economical. The present research focuses on the biosurfactant production from Pseudomonas mosselii utilizing Parthenium hysterophorus as a relatively cheap substrate. P. hysterophorus being a hazardous weed, its eradication is quite tedious. So, the utilization of the weed for useful purposes serves as a choice to overcome the problems posed by the weed. In the study, this weed has been successfully utilized as a substrate and the optimized fermentative production of biosurfactant was done. From one—factor-at a—time analysis it was known that the substrate level of 3% incubation time of 96 h, pH 6.0, temperature 35 °C, glucose and yeast extract was found to be the best C and N sources for a high yield. The extracted biosurfactant was partially purified and characterized using FTIR. The biosurfactant produced from the weed could help to render the milestone for distinct biomedical and other applications.

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References

  • Anzai Y, Kim H, Park J-Y et al (2000) Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol 50:1563–1589

    Article  CAS  PubMed  Google Scholar 

  • Aparna A, Srinikethan G, Smitha H (2012) Production and characterization of biosurfactant produced by a novel Pseudomonas sp. 2B. Colloids Surf B 95:23–29

    Article  CAS  Google Scholar 

  • Bajwa AA, Chauhan BS, Adkins S (2017) Morphological, physiological and biochemical responses of two Australian biotypes of Parthenium hysterophorus to different soil moisture regimes. Environ Sci Pollut Res 24:16186–16194

    Article  CAS  Google Scholar 

  • Banat IM, Makkar RS, Cameotra SS (2000) Potential commercial applications of microbial surfactants. Appl Microbiol Biotechnol 53:495–508

    Article  CAS  PubMed  Google Scholar 

  • Cameotra SS, Makkar RS (2004) Recent applications of biosurfactants as biological and immunological molecules. Curr Opin Microbiol 7:262–266

    Article  CAS  PubMed  Google Scholar 

  • Channappagoudar BB, Biradar NR, Patil JB, Gasimani CAA (2007) Utilization of weed biomass as an organic source in sorghum. Karnataka J Agric Sci 20(2):245–248

    Google Scholar 

  • Costa GAN (2005) Biotechnological production of surfactant agro-industrial residue in Bacillus subtilis, characterization and application. Campinas, Dissertation-(Master in FoodScience), Faculty of Food Engineering, State University of Campinas-Unicamp. 2005: 87

  • Dabboussi F, Hamze M, Singer E et al (2002) Pseudomonas mosselii sp. nov., a novel species isolated from clinical specimens. Int J Syst Evol Microbiol 52:363–376

    Article  CAS  PubMed  Google Scholar 

  • Das D, Ashoka D, Aradhya R, Inamdar M (2008) Gene expression analysis in post-embryonic pericardial cells of Drosophila. Gene Expr Patterns 8:199–205

    Article  CAS  PubMed  Google Scholar 

  • de Quadros CP, Duarte MCT, Pastore GM (2011) Biological activities of a mixture of biosurfactant from Bacillus subtilis and alkaline lipase from Fusarium oxysporum. Braz J Microbiol 42:354–361

    Article  Google Scholar 

  • Desai JD, Banat IM (1997) Microbial production of surfactants and their commercial potential. Microbiol Mol Biol Rev 61:47–64

    CAS  PubMed  PubMed Central  Google Scholar 

  • Dubey K, Juwarkar A (2001) Distillery and curd whey wastes as viable alternative sources for biosurfactant production. World J Microbiol Biotechnol 17(1):61–69

    Article  CAS  Google Scholar 

  • Elazzazy AM, Abdelmoneim TS, Almaghrabi OA (2015) Isolation and characterization of biosurfactant production under extreme environmental conditions by alkali-halo-thermophilic bacteria from Saudi Arabia. Saudi J Biol Sci 22:466–475

    Article  CAS  PubMed  Google Scholar 

  • Folch J, Lees M, Sloane Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–509

    CAS  Google Scholar 

  • Ghribi D, Ellouze-Chaabouni S (2011) Enhancement of Bacillus subtilis lipopeptide biosurfactants production through optimization of medium composition and adequate control of aeration. Biotechnol Res Int 2011:1–6

    Article  CAS  Google Scholar 

  • Gudiña EJ, Teixeira JA, Rodrigues LR (2011) Biosurfactant-producing lactobacilli: screening, production profiles, and effect of medium composition. Appl Environ Soil Sci. https://doi.org/10.1155/2011/201254

    Article  Google Scholar 

  • Gurib-Fakim A, Sewraj MD, Gueho J, Dulloo E (1996) Medicinal plants of Rodrigues. Int J Pharmacogn 34:2–14

    Article  Google Scholar 

  • Hassanshahian M (2014) Isolation and characterization of biosurfactant producing bacteria from Persian Gulf (Bushehr provenance). Mar Pollut Bull 86:361–366

    Article  CAS  PubMed  Google Scholar 

  • Ibrahim ML, Ijah UJJ, Manga SB et al (2013) Production and partial characterization of biosurfactant produced by crude oil degrading bacteria. Int Biodeterior Biodegradation 81:28–34

    Article  CAS  Google Scholar 

  • Ismail HY, Ijah UJJ, Riskuwa ML, Allamin II (2014) Biodegradation of spent engine oil by bacteria isolated from the rhizosphere of legumes grown in contaminated soil. Int J Environ 3:63–75

    Article  Google Scholar 

  • Jain DK, Collins-Thompson DL, Lee H, Trevors JT (1991) A drop-collapsing test for screening surfactant-producing microorganisms. J Microbiol Methods 13:271–279

    Article  Google Scholar 

  • Marimuthu K, Ravi D (2014) Phytochemical analysis of Parthenium hysterophorus L. leaf. World J Pharm Res 3(6):1066–1074

    Google Scholar 

  • Mukherjee S, Das P, Sivapathasekaran C, Sen R (2008) Enhanced production of biosurfactant by a marine bacterium on statistical screening of nutritional parameters. Biochem Eng J 42:254–260

    Article  CAS  Google Scholar 

  • Mulligan CN (2005) Environmental applications for biosurfactants. Environ Pollut 133:183–198

    Article  CAS  Google Scholar 

  • Oussou KR, Yolou S, Boti JB, Guessennd KN, Kanko C, Ahibo C, Casanova J (2008) Chemical study and ant diarrheal activity of the essential oils of two aromatic plants of the Ivorian pharmacopoeia. Eur J Sci Res 24:94–103

    Google Scholar 

  • Padmavathi AR, Pandian SK (2014) Antibiofilm activity of biosurfactant producing coral associated bacteria isolated from Gulf of Mannar. Indian J Microbiol 54:376–382

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Pereira JFB, Gudiña EJ, Costa R et al (2013) Optimization and characterization of biosurfactant production by Bacillus subtilis isolates towards microbial enhanced oil recovery applications. Fuel 111:259–268

    Article  CAS  Google Scholar 

  • Płaza GA, Pacwa-Płociniczak Magdalena, Piotrowska-Seget Z, Jangid K, Wilk KA (2011) Agroindustrial wastes as unconventional substrates for growing of Bacillus strains and production of biosurfactant. Environ Prot Eng 37:63–71

    Google Scholar 

  • Pumiput P, Chuntranuluck S, Kitpreechavanich V, Pilanee Vaithanomsat VP (2008) Production process of hydrolysate from steam explosion of oil palm trunk for xylitol fermentation. Kasetsart J 42:73

    CAS  Google Scholar 

  • Sabapathy PC, Devaraj S, Kathirvel P (2017) Parthenium hysterophorus: low cost substrate for the production of polyhydroxyalkanoates. Curr Sci 112:2106–2111

    Article  CAS  Google Scholar 

  • Saharan BS, Sahu RK, Sharma D (2011) A review on biosurfactants: fermentation, current developments and perspectives. Genet Eng Biotechnol J 2011:1–14

    Google Scholar 

  • Silva EJ, e Silva NMPR, Rufino RD et al (2014) Characterization of a biosurfactant produced by Pseudomonas cepacia CCT6659 in the presence of industrial wastes and its application in the biodegradation of hydrophobic compounds in soil. Colloids Surf B Biointerfaces 117:36–41

    Article  CAS  PubMed  Google Scholar 

  • Vaz DA, Gudina EJ, Alameda EJ et al (2012) Performance of a biosurfactant produced by a Bacillus subtilis strain isolated from crude oil samples as compared to commercial chemical surfactants. Colloids Surf B Biointerfaces 89:167–174

    Article  CAS  PubMed  Google Scholar 

  • Wikler MA (2005). Methods for dilution for antimicrobial susceptibility tests for bacteria that grown aerobically. Clin. Laboratorial Standards Inst.—CLSI. M7-A6, 23

  • Wittgens A, Tiso T, Arndt TT et al (2011) Growth independent rhamnolipid production from glucose using the non-pathogenic Pseudomonas putida KT2440. Microb Cell Fact 10:80

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

Author Devaraj Sabarinathan, greatly thank G. Kalaiarasi and K.Murali Research scholar, Department of Chemistry, Bharathiar University for her valuable guidance in the spectroscopic analysis. Nehru Lavanya is thankful to the Department of Science and Technology ((DST- PURSE) Government of India) for the Financial Support throughout project.

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Authors and Affiliations

  1. Biopharmacy Lab, Department of Microbial Biotechnology, Bharathiar University, Coimbatore, 641046, Tamil Nadu, India

    Sabarinathan Devaraj, Poorna Chandrika Sabapathy, Lavanya Nehru & Kathirvel Preethi

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  1. Sabarinathan Devaraj
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  2. Poorna Chandrika Sabapathy
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  3. Lavanya Nehru
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  4. Kathirvel Preethi
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Correspondence to Sabarinathan Devaraj.

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Devaraj, S., Sabapathy, P.C., Nehru, L. et al. Bioprocess optimization and production of biosurfactant from an unexplored substrate: Parthenium hysterophorus. Biodegradation 30, 325–334 (2019). https://doi.org/10.1007/s10532-019-09878-7

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  • DOI: https://doi.org/10.1007/s10532-019-09878-7

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