Abstract
Pseudomonas strains isolated from oil contaminated soils were screened for biosurfactant production. Three out of eleven Pseudomonas isolates were selected for their high emulsifying activity (E24 value on n-hexadecane ~ 78%). These isolates (E39, E311 and E313) were identified as members of the P. putida group using phenotypical methods and a molecular approach. To identify the chemical nature of produced biosurfactants, thin layer chromatography and MALDI-ToF mass spectrometry analysis were carried out and revealed lipopeptides belonging to the syringafactin family. The activity of the produced biosurfactants was stable over a pH range of 6–12, at high salinity (10%) and after heating at 80 °C. Tests in contaminated sand micro-bioreactors showed that the three strains were able to degrade diesel. These results suggest the potential of these syringafactin producing strains for application in hydrocarbon bioremediation.
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Acknowledgements
This work was funded by the Ministry of Higher Education and Scientific Research of Tunisia and a scholarship from the region Hauts-de-France. This work was supported by the European Union SMARTBIOCONTROLPORTFOLIO-Interreg FWVLBIOPROD program, the FEDER program ALIBIOTECH and the CPER project ALIBIOTECH from the region Hauts-de-France. The authors are grateful to the Advanced High Throughput Technologies Platform for Biorefineries Catalysts Design ‘REALCAT’ for allowing the implementation of MALDI-ToF mass spectrometry analysis. The authors are grateful to Kamel Maaloul, translator and English professor for having proofread the manuscript.
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Zouari, O., Lecouturier, D., Rochex, A. et al. Bio-emulsifying and biodegradation activities of syringafactin producing Pseudomonas spp. strains isolated from oil contaminated soils. Biodegradation 30, 259–272 (2019). https://doi.org/10.1007/s10532-018-9861-x
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DOI: https://doi.org/10.1007/s10532-018-9861-x