Abstract
Response surface methodology was applied to optimize the production of biosurfactants from Bacillus mojavensis I4 using Box–Behnken design with four variables. The optimal variable combination was 3% of glucose as carbon source, 0.6% of glutamic acid as nitrogen source, temperature of 35 °C and 10 g/l of NaCl which yielded to an optimal production of 4.12 g/l. Compositional analysis and FTIR spectrum revealed that the extracted biosurfactants was a lipopeptides. The biosurfactants achieved a critical micelle concentration value of 100 mg/l. Moreover, the extracted biosurfactants were effective at recovering up to 89.2% of motor oil from sand beach and achieved a dispersion rate of 78% of the initial diameter of the oil. These findings suggested the potential use of I4 biosurfactants in the oil industry.
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This work was funded by the Tunisian MESRS (Ministère de l’Enseignement Supérieur et de la Recherche Scientifique).
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Ghazala, I., Bouallegue, A., Haddar, A. et al. Characterization and production optimization of biosurfactants by Bacillus mojavensis I4 with biotechnological potential for microbial enhanced oil recovery. Biodegradation 30, 235–245 (2019). https://doi.org/10.1007/s10532-018-9844-y
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DOI: https://doi.org/10.1007/s10532-018-9844-y