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Integration of Biochemical, Cellular, and Genetic Indicators for Understanding the Aging Process in a Bivalve Mollusk Chlamys farreri

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Abstract

The major causal factors for the irreversible decline in physical vitality during organismal aging are postulated to be a chronic state of cellular redox imbalance, metabolic toxicity, and impaired energy homeostasis. We assessed whether the relevant enzyme activity, oxidative stress, and intracellular ATP might be causally involved in the aging of short-lived Chlamys farreri (life span 4~5 years). A total of eight related biochemical and cellular indicators were chosen for the subsequent analysis. All the indicators were measured in seven different tissues from scallops aged one to four years, and our data support that the aging of C. farreri is associated with attenuated tissue enzyme activity as well as a decreased metabolic rate. Through principal component analysis, we developed an integrated vigor index for each tissue for comprehensive age-related fitness evaluation. Remarkably, all tissue-integrated vigor indexes significantly declined with age, and the kidney was observed to be the most representative tissue. Further transcriptional profiling of the enzymatic genes provided additional detail on the molecular responses that may underlie the corresponding biochemical results. Moreover, these critical molecular responses may be attributed to the conserved hierarchical regulators, e.g., FOXO, AMPKs, mTOR, and IGF1R, which were identified as potentially novel markers for chronic fitness decline with age in bivalves. The present study provides a systematic approach that could potentially benefit the global assessment of the aging process in C. farreri and provide detailed evaluation of the biochemical, cellular, and genetic indicators that might be involved. This information may assist in a better understanding of bivalve adaptability and life span.

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Data Availability

The gene sequences used in the present research can be obtained from cfBase (http://mgb.ouc.edu.cn/cfbase/cgi-bin/blast) and NCBI (https://www.ncbi.nlm.nih.gov/).

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Acknowledgments

We acknowledge the grant support from the National Natural Science Foundation of China (U1706203), the Fundamental Research Funds for the Central Universities (201841001), the key Research and Development Program of Shandong Province (2016ZDJQ0208), Youth Talent Program Supported by Laboratory for Marine Fisheries Science and Food Production Processes (2018-MFS-T06) and Taishan Scholar Project Fund of Shandong Province of China.

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  1. Shanshan Lian and Jing Wang contributed equally to this work.

Authors and Affiliations

  1. MOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao, 266003, China

    Shanshan Lian, Jing Wang, Lingling Zhang, Qiang Xing, Naina Hu, Sinuo Liu, Xiaoting Dai, Fengmei Zhang, Xiaoli Hu, Zhenmin Bao & Shi Wang

  2. Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China

    Shanshan Lian, Lingling Zhang & Shi Wang

  3. Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China

    Qiang Xing, Xiaoli Hu & Zhenmin Bao

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  1. Shanshan Lian
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Lian, S., Wang, J., Zhang, L. et al. Integration of Biochemical, Cellular, and Genetic Indicators for Understanding the Aging Process in a Bivalve Mollusk Chlamys farreri. Mar Biotechnol 21, 718–730 (2019). https://doi.org/10.1007/s10126-019-09917-7

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