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NMR assignment of free 1H, 15N and 13C-Grb2-SH2 domain

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Abstract

Growth factor receptor-bound protein 2 (Grb2) is an adaptor protein composed of three domains, an N-terminal SH3 (nSH3), SH2 and a C-terminal SH3 (cSH3) domains. This multi-domain protein has been reported to be a key factor in many signaling pathways related to controlling cell survival, differentiation, and growth. The Grb2-SH2 domain has been a focus for the study of the interaction with peptides and small molecules to act as inhibitors in uncontrolled cell growth, and consequently inhibit tumor proliferation. Here we describe the almost complete assignment of the free SH2 domain at pH 7. This work prepares the ground for further structural studies, backbone dynamics, mapping of interactions and drug screening and development. TalosN secondary structure prediction showed great similarity with the available structures in the PDB.

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Acknowledgements

This work was supported by FAPESP Grants Nos. (2014/17630-0, 2017/20642-8), CNPq Grants No. 442951/2014-0, UNESP-PROPG Grants Nos. (09/2017, 12/2017) awarded to FAM; FAPESP Grants No. 2009/53989-4; and by FAPERJ Grants Nos. (239229, 204432) and CNPq Grants No. 309564/2017-4 awarded to FCLA. We thank NMRbox: National Center for Biomolecular NMR Data Processing and Analysis, a Biomedical Technology Research Resource (BTRR), which is supported by NIH Grants No. P41GM111135 (NIGMS). We also thank INBEB-INCT for funding. The assignment was deposit at the Biomagnetic Resonance Data Bank (BMRB ID 27781). KS is funded by CNPq scholarship (131968/2017-3) and IPC is funded by a postdoctoral fellowship by FAPERJ.

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Authors and Affiliations

  1. Multiuser Center for Biomolecular Innovation (CMIB), Department of Physics, São Paulo State University (UNESP), São José Do Rio Preto, São Paulo, Brazil

    Karoline Sanches, Ícaro P. Caruso & Fernando A. Melo

  2. Institute of Medical Biochemistry (IBqM) and National Center of Nuclear Magnetic Resonance (CNRMN), Center for Structural Biology and Bioimaging (CENABIO), Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

    Ícaro P. Caruso & Fábio C. L. Almeida

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  1. Karoline Sanches
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  2. Ícaro P. Caruso
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  3. Fábio C. L. Almeida
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Correspondence to Fábio C. L. Almeida or Fernando A. Melo.

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Sanches, K., Caruso, Í.P., Almeida, F.C.L. et al. NMR assignment of free 1H, 15N and 13C-Grb2-SH2 domain. Biomol NMR Assign 13, 295–298 (2019). https://doi.org/10.1007/s12104-019-09894-x

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  • DOI: https://doi.org/10.1007/s12104-019-09894-x

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