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The Sponge-Associated Fungus Eurotium chevalieri MUT 2316 and its Bioactive Molecules: Potential Applications in the Field of Antifouling

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Abstract

The need for new environmentally friendly antifouling and the observation that many marine organisms have developed strategies to keep their surface free of epibionts has stimulated the search for marine natural compounds with antifouling activities. Sponges and in particular fungi associated with them represent one of the most appropriate sources of defence molecules and could represent a promising biomass for the supply of new antifouling compounds. The objective of this work was therefore to evaluate the antifouling potency of 7 compounds isolated from the sponge derived fungus Eurotium chevalieri MUT 2316. The assessment of their activity targeted the inhibition of the adhesion and/or growth of selected marine bacteria (5) and microalgae (5), as well as the inhibition of the mussel’s byssus thread formation (tyrosinase activity). The 7 compounds showed bioactivity, with various levels of selectivity for species. Cyclo-L-Trp-L-Ala was the most promising active compound, and led to the inhibition, at very low concentrations (0.001 μg ml−1 in 61.5% of cases), of adhesion and growth of all the microalgae, of selected bacteria, and towards the inhibition of tyrosinase. Promising results were also obtained for echinulin, neoechinulin A, dihydroauroglaucin and flavoglaucin, respectively, leading to inhibition of adhesion and/or growth of 9, 7, 8 and 8 microfouling species at various concentrations.

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Funding

This research was supported by the Galileo project [grant number G18-701] and the Vinci project [grant number C2-22] from the Italo-French University. Biodimar team was supported by funding from Biogenouest. M. Mehiri is supported by the EMBRIC project (EU grant No 654008) and the Galileo project from the Italo-French University [grant number 34595SA]. This work was supported by the French government, managed by the French National Research Agency under the project Investissements d’Avenir UCAJEDI (# ANR-15-IDEX-01).

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  1. Mycotheca Universitatis Taurinensis, Department of Life Sciences and Systems Biology, University of Turin, Viale Mattioli 25, 10125, Turin, Italy

    Elena Bovio & Giovanna Cristina Varese

  2. CNRS, Nice Institute of Chemistry, UMR 7272, Marine Natural Products Team, University Nice Côte d’Azur, 60103, Nice, France

    Elena Bovio & Mohamed Mehiri

  3. University Brest, CNRS, IRD, Ifremer, LEMAR, Institut Universitaire Européen de la Mer, F-29280, Plouzané, France

    Marilyne Fauchon, Yannick Toueix & Claire Hellio

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  1. Elena Bovio
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  2. Marilyne Fauchon
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  5. Giovanna Cristina Varese
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  6. Claire Hellio
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Correspondence to Giovanna Cristina Varese or Claire Hellio.

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Bovio, E., Fauchon, M., Toueix, Y. et al. The Sponge-Associated Fungus Eurotium chevalieri MUT 2316 and its Bioactive Molecules: Potential Applications in the Field of Antifouling. Mar Biotechnol 21, 743–752 (2019). https://doi.org/10.1007/s10126-019-09920-y

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