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Tunneling nanotubes mediate intercellular communication between endothelial progenitor cells and osteoclast precursors

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Abstract

Tunneling nanotube (TNT)-mediated cell communication play pivotal roles in a series of physiological and pathological processes in multicellular organism. This study was designed to investigate the existence of TNTs between EPCs and osteoclast precursors and evaluate their effects on the differentiation of osteoclast precursors. For these purposes, EPCs and osteoclast precursors (RAW264.7 cells) were stained with different fluorescent dyes before direct co-culture; then, the co-cultured cells were sorted by fluorescence activated cell sorter (FACS), and the differentiation of co-cultured RAW264.7 cells was evaluated. The results showed that the differentiation potential of RAW264.7 cells was significantly inhibited after their co-culture with EPCs. Additionally, the expression of macrophage migration inhibitory factor (MIF) was up-regulated in RAW264.7 cells after co-culture. Moreover, the MIF inhibitor ISO-1 could rescue the formation of TRAP-positive multinuclear osteoclasts and the expression of osteoclastogenesis-associated genes in the co-cultured RAW264.7 cells. The present study demonstrates that EPCs can affect the differentiation of osteoclast precursors through the TNT-like structures formed across these two types of cells and might inform new therapeutic strategies for osteolytic diseases.

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Abbreviations

TNT:

Tunneling nanotube

EPCs:

Endothelial progenitor cells

MIF:

Macrophage migration inhibitory factor

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Funding

This study was supported by the National Natural Science Foundation of China (81570994), National Natural Science Foundation of China (81800946) and National Natural Science Foundation of China (81602780).

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Authors and Affiliations

  1. State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, Wuhan University, Wuhan, China

    Rui-Fang Li, Wei Zhang, Qi-Wen Man & Yi-Fang Zhao

  2. Department of Oral and Maxillofacial Surgery, Wuhan University, Wuhan, China

    Rui-Fang Li, Wei Zhang, Qi-Wen Man & Yi-Fang Zhao

  3. State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, and Department of Prosthodontics, School & Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan, 430079, China

    Yi Zhao

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  1. Rui-Fang Li
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  2. Wei Zhang
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  4. Yi-Fang Zhao
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Correspondence to Yi Zhao.

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Li, RF., Zhang, W., Man, QW. et al. Tunneling nanotubes mediate intercellular communication between endothelial progenitor cells and osteoclast precursors. J Mol Hist 50, 483–491 (2019). https://doi.org/10.1007/s10735-019-09842-y

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  • DOI: https://doi.org/10.1007/s10735-019-09842-y

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