Abstract
The claustrum is a subcortical nucleus, found in the telencephalon of all placental mammals. Earlier Golgi studies have mostly focused on a qualitative description of the types of neurons. The aim of the present study was to describe the types of neurons found in the dorsal claustrum of the cat using the Golgi impregnation method and to perform a quantitative analysis of the following morphometric parameters: number of terminals (ends), total dendritic length, dendritic complexity, spine density (in spiny projection neurons), varicosity density (in aspiny interneurons). We used specimens from 5 healthy male cats stained according to the Golgi-Cox method. The dendritic trees of the studied neurons were then reconstructed through the Neurolucida software. Values of the studied quantitative parameters were obtained automatically and tested for statistically significant differences. Five types of spiny neurons were observed—large, medium-sized and small multipolar, bipolar and pyramidal-like. In addition, we described three types of aspiny neurons. The quantitative values and the statistical analysis were presented with tables and diagrams. In conclusion, we have presented a detailed analysis of the cytoarchitecture of the DC of the cat and have reported the first quantitative data on a number of morphometric parameters.
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Acknowledgements
The authors of the present manuscript would like to express their most sincere gratitude to Assoc. Prof. Todor Kundurzhiev, PhD, of the Medical University of Sofia, Bulgaria for his valuable assistance in the preparation of the statistical analysis. We would also like to thank Assoc. Prof. Jordanka Angelova, PhD, of the University of Chemical Technology and Metallurgy, Sofia, Bulgaria for her help in the preparation of the figures related to the statistical analysis.
This work is supported by the Bulgarian Ministry of Education and Science under the National Program for Research ‘Young Scientists and Postdoctoral Students’.
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Hinova-Palova, D., Kotov, G., Landzhov, B. et al. Cytoarchitecture of the dorsal claustrum of the cat: a quantitative Golgi study. J Mol Hist 50, 435–457 (2019). https://doi.org/10.1007/s10735-019-09839-7
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DOI: https://doi.org/10.1007/s10735-019-09839-7