Skip to main content
SpringerLink
Log in

NSP Protein Encoded in Negative NS RNA Strand of Influenza A Virus Induces Cellular Immune Response in Infected Animals

  • BIOCHEMISTRY, BIOPHYSICS, AND MOLECULAR BIOLOGY
  • Published:
Doklady Biochemistry and Biophysics Aims and scope Submit manuscript

Abstract

Infection of mice with influenza A viruses led to the formation of clones of lymphocytes that specifically recognizes viral domains in the central zone of the NSP protein (amino acid positions 83–119). Computer analysis of the primary structure of the NSP protein showed the presence of T-cell epitopes in the central part of the NSP molecule. The findings indicate that the viral NSP gene is expressed in the infected animals and verify the concept of the bipolar strategy (ambisense strategy) of the influenza A virus genome.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.

Similar content being viewed by others

REFERENCES

  1. Dou, D., Revol, R., Ostbye, H., Wang, H., and Daniels, R., Influenza A virus cell entry, replication, virion assembly and movement, Front. Immunol., 2018, vol. 9, p. 1581. https://doi.org/10.3389/fimmu.2018.01581

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Palese Baez, M., Zazra, J.J., Elliott, R.M., Young, J.F., and Palese, P., Nucleotide sequence of the influenza A/duck/Alberta/60/76 virus NS RNA: conservation of the NS1/NS2 overlapping gene structure in a divergent influenza virus RNA segment, Virology, 1981, vol. 113, no. 1, pp. 397–402.

    Article  PubMed  Google Scholar 

  3. Zhirnov, O.P., Poyarkov, S.V., Vorob’eva, I.V., Safonova, O.A., Malyshev, N.A., and Klenk, N.D., Segment NS of influenza A virus contains an additional gene NSP in positive-sense orientation, Dokl. Biochem. Biophys., 2007, vol. 414, pp. 127–133. PMID .17695319

    Article  CAS  PubMed  Google Scholar 

  4. Zhirnov, O.P., Vorobjeva, I.V., Saphonova, O.A., Poyarkov, S.V., Ovcharenko, A.V., Anhlan, D., and Malyshev, N.A., Structural and evolutionary characteristics of HA, NA, NS, and M genes of clinical influenza A/H3N2 viruses passaged in human and canine cells, J. Clin. Virol., 2009, vol. 45, no. 4, pp. 322–333. https://doi.org/10.1016/j.jcv.2009.05.030

    Article  CAS  PubMed  Google Scholar 

  5. Zhirnov, O.P., Akulich, K.A., Lipatova, A.V., and Usachev, E.V., Negative-sense virion RNA of segment 8 (NS) of influenza A virus is able to translate in vitro a new viral protein, Dokl. Biochem. Biophys., 2017, vol. 473, pp. 122–127. https://doi.org/10.1134/S1607672917020090

    Article  CAS  PubMed  Google Scholar 

  6. Clifford, M., Twigg, J., and Upton, C., Evidence for a novel gene associated with human influenza A viruses, Virol. J., 2009, vol. 6, p. 198. https://doi.org/10.1186/1743-422X-6-198

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Gong, Y.N., Chen, G.W., Chen, C.J., Kuo, R.L., and Shih, S.R., Computational analysis and mapping of novel open reading frames in influenza A viruses, PLoS One, 2014, vol. 9, no. 12. e115016. https://doi.org/10.1371/journal.pone.0115016

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Yang, C.W. and Chen, M.F., Uncovering the potential pan proteomes encoded by genomic strand RNAs of influenza A viruses, PLoS One, 2016, vol. 11, no. 1. e0146936. https://doi.org/10.1371/journal.pone.0146936

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Sabath, N., Morris, J.S., and Graur, D., Is there a twelfth protein-coding gene in the genome of influenza A? A selection-based approach to the detection of overlapping genes in closely related sequences, J. Mol. Evol., 2011, vol. 73, nos. 5–6, pp. 305–315. https://doi.org/10.1007/s00239-011-9477-9

    Article  CAS  PubMed  Google Scholar 

  10. Jensen, K.K., Andreatta, M., Marcatili, P., Buus, S., Greenbaum, J.A., Yan, Z., Sette, A., Peters, B., and Nielsen, M., Improved methods for predicting peptide binding affinity to MHC class II molecules, Immunology, 2018, vol. 154, no. 3, pp. 394–406. https://doi.org/10.1111/imm.12889

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Bhasin, M. and Raghava, G.P.S., A hybrid approach for predicting promiscuous MHC class I restricted T cell epitopes, J. Biosci., 2006, vol. 32, pp. 31–42.

    Article  Google Scholar 

  12. Jameson, B.A. and Wolf, H., The antigenic index: a novel algorithm for predicting antigenic determinants, Comput. Appl. Biosci., 1988, vol. 4, no. 1, pp. 181–186.

    CAS  PubMed  Google Scholar 

  13. Panthu, B., Terrier, O., Carron, C., Traversier, A., Corbin, A., Balvay, L., Lina, B., Rosa-Calatrava, M., and Ohlmann, T., The NS1 protein from influenza virus stimulates translation initiation by enhancing ribosome recruitment to mRNAs, J. Mol. Biol., 2017, vol. 429, no. 21, pp. 3334–3352. https://doi.org/10.1016/j.jmb.2017.04.007

    Article  CAS  PubMed  Google Scholar 

  14. Zhong, W., Reche, P.A., Lai, C.C., Reinhold, B., and Reinherz, E.L., Genome-wide characterization of a viral cytotoxic T lymphocyte epitope repertoire, J. Biol. Chem., 2003, vol. 278, no. 46, pp. 45135–45144.

    Article  CAS  PubMed  Google Scholar 

  15. Nguyen, M. and Haenni, A.L., Expression strategies of ambisense viruses, Virus Res., 2003, vol. 93, no. 2, pp. 141–150.

    Article  CAS  PubMed  Google Scholar 

Download references

Funding

This work was partially supported by the Russian Foundation for Basic Research (project nos. 16-04-01271, 13-04-001824) and the Scientific Program SFB 293 of the German Research Society (DFG).

Author information

Authors and Affiliations

  1. Ivanovsky Institute of Virology, Gamaleya Research Center of Epidemiology and Microbiology, Ministry of Healthcare of the Russian Federation, 123098, Moscow, Russia

    O. P. Zhirnov & E. I. Isaeva

  2. Russian–German Academy of Medical and Biotechnological Sciences, 142782, Moscow, Russia

    O. P. Zhirnov

Authors
  1. O. P. Zhirnov
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. I. Isaeva
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to O. P. Zhirnov.

Ethics declarations

Conflict of interests. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Additional information

Translated by M. Batrukova

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhirnov, O.P., Isaeva, E.I. NSP Protein Encoded in Negative NS RNA Strand of Influenza A Virus Induces Cellular Immune Response in Infected Animals. Dokl Biochem Biophys 486, 201–205 (2019). https://doi.org/10.1134/S1607672919030128

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1607672919030128

Search

Navigation