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Study of dCTCF Insulator Activity in Drosophilamelanogaster Model Systems

  • BIOCHEMISTRY, BIOPHYSICS, AND MOLECULAR BIOLOGY
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Abstract

Using transgenic Drosophila model systems, we showed that four binding sites for the architectural protein dCTCF per se cannot form an effective insulator that blocks enhancers and protects against the Polycomb-dependent repression. These results suggest that, in the known Drosophila insulators, the dCTCF protein functions in cooperation with other architectural proteins.

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Funding

The study was supported by the Russian Foundation for Basic Research (project no. 19-04-00340).

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Authors and Affiliations

  1. Institute of Gene Biology, Russian Academy of Sciences, 119334, Moscow, Russia

    N. E. Postika, T. A. Ivlieva,  P. G. Georgiev & O. V. Kyrchanova

Authors
  1. N. E. Postika
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  2. T. A. Ivlieva
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  3. P. G. Georgiev
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  4. O. V. Kyrchanova
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Corresponding author

Correspondence to N. E. Postika.

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Conflict of interests. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Translated by M. Batrukova

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Postika, N.E., Ivlieva, T.A., Georgiev, P.G. et al. Study of dCTCF Insulator Activity in Drosophilamelanogaster Model Systems. Dokl Biochem Biophys 486, 187–191 (2019). https://doi.org/10.1134/S1607672919030074

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  • DOI: https://doi.org/10.1134/S1607672919030074

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