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SPECT/CT imaging of apoptosis in aortic aneurysm with radiolabeled duramycin

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A Correction to this article was published on 17 August 2019

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Abstract

The objective of this research was to estimate whether a [99mTc]duramycin probe can be used for apoptosis imaging in patients with aortic aneurysm (AA). Vascular smooth muscle cell (SMC) apoptosis has an important influence on AA development. Thus, non-invasive imaging of SMC apoptosis may be able to evaluate AA progress and risk stratification. SMCs were treated with hydrogen peroxide (H2O2; 200 μΜ) or culture medium as a control. Apoptosis was measured using flow cytometry and [99mTc]duramycin to detect the binding efficiency to apoptotic SMCs. C57/BL6 mice were administered angiotensin-II and beta-aminopropionitrile (BAPN) subcutaneously to establish an AA model, or saline for controls. Aortic specimens underwent pathological evaluation and their aortic diameters were measured after 6 weeks. Micro-SPECT/CT scanning of [99mTc]duramycin and 18F-FDG PET detection were performed. SMCs treated with H2O2 showed more apoptosis compared with the control group (67.2 ± 3.8% vs. 16.1 ± 0.6%, P < 0.01). The experimental group showed a high rate of AA formation (70%) compared with no AA formation in the control group. The average aorta diameter was higher and [99mTc]duramycin uptake at the AA site was higher in the experimental group compared with the control group. Compared with the normal aorta in the control group, AA in experiment group had more severe medial degeneration, elastic fiber reduction and fracture, and collagen degeneration. TUNEL staining verified the higher apoptosis rate at the AA site in experiment group compared with the control group (63.9 ± 3.7% in ascending AA, 66.4 ± 4.0% in thoracic AA, vs. 3.5 ± 0.3% in normal aorta, P < 0.01). [99mTc]Duramycin may be an effective probe to evaluate apoptosis in AA.

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  • 17 August 2019

    The original version of this article unfortunately contains errors in Figure 4. An incorrect Figure 4D is published which is actually a repetition of Figure 2C (i.e., apoptosis rate in control vs. H2O2-treated group). The correct Figure 4D should be the aortic diameter of control vs. experimental groups. Also, the order of part figures (a\b\c\d) in Figure 4E is incorrect. The correct Figure 4 is given below.

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Acknowledgements

The authors acknowledge the funding from National Natural Science Foundation of China (Grant No. 81771971), Shanghai Pujiang Program (Grant No. 17PJ1401500), Shanghai Municipal Health Commission (Grant No. 201640134), and the “Chen Guang” Project Supported by Shanghai Municipal Education Commission and Shanghai Education Development Foundation (Grant No. 14CG06).

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Author notes

  1. Chengkai Hu and Hui Tan have contributed equally to this work.

Authors and Affiliations

  1. Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, 1609 Xietu Road, Shanghai, 200032, China

    Chengkai Hu, Mieradilijiang Abudupataer, Yun Zhao, Jun Li, Jiawei Gu, Chunsheng Wang, Kai Zhu & Hao Lai

  2. Shanghai Institute of Cardiovascular Diseases, Shanghai, 200032, China

    Chengkai Hu, Mieradilijiang Abudupataer, Yun Zhao, Jun Li, Jiawei Gu, Chunsheng Wang, Kai Zhu & Hao Lai

  3. Department of Nuclear Medicine, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai, 200032, China

    Hui Tan, Qingyu Lin & Dengfeng Cheng

  4. Institute of Nuclear Medicine, Fudan University, Shanghai, 200032, China

    Hui Tan, Qingyu Lin & Dengfeng Cheng

  5. Shanghai Institute of Medical Imaging, Shanghai, 200032, China

    Hui Tan, Qingyu Lin & Dengfeng Cheng

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Hu, C., Tan, H., Lin, Q. et al. SPECT/CT imaging of apoptosis in aortic aneurysm with radiolabeled duramycin. Apoptosis 24, 745–755 (2019). https://doi.org/10.1007/s10495-019-01554-8

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