Abstract
Plants respond to environmental stress with a number of physiological and developmental changes. Water deficit is one of the major factors limiting plant growth and development and crop productivity. One response of plants to water deficit is accumulation of abscisic acid (ABA). An increase of ABA is responsible for the induction of many genes, presumably some of which contribute to drought tolerance. Analysis of gene expression in barley seedling shoots by differential display reverse transcriptase polymerase chain reaction (DDRT-PCR) led to the isolation of several drought-, cold- and ABA-induced partial cDNA fragments. Here we extensively characterize one of these cDNAs, designated DD6. First, a larger cDNA was extended from DD6 by 5′-RACE (rapid amplification of cDNA ends). Subsequently, the corresponding gene was isolated by screening a barley BAC library, and the sequences of the transcribed and flanking regions were determined. The deduced amino acid sequence has similarity to an Arabidopsis hypothetical protein and to a human and mouse DNA-binding protein. The corresponding gene, named Srg6 (stress-responsive gene), was mapped in a barley doubled haploid mapping population to chromosome 7H between markers ABC455 and salfp76, within a region that previously has been linked to osmotic adaptation in barley and other grass genomes.
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Abe, H., Yamaguchi-Shinozaki, K., Urao, T., Iwasaki, T., Hosokawa, D. and Shinozaki, K. 1997. Role of Arabidopsis MYC and MYB homologs in drought and abscisic acid regulated gene expression. Plant Cell 9: 1859–1868.
Altschul, S.F., Madden, T.L., Schäffer, A.A., Zhang, J.H., Zhang, Z., Miller, W. and Lipman, D.J. 1997. Gapped BLAST and PSIBLAST: a new generation of protein database search programs. Nucl. Acids Res. 25: 3389–3402.
Baker, J., Steel, C. and Dure, L. 1988. Sequence and characterization of 6 Lea proteins and their genes from cotton. Plant Mol. Biol. 11: 277–291.
Champoux, M.C., Wang, G., Sarkarung, S., Mackill, D.J., O'Toole, J.C., Huang, N. and McCouch, S.R. 1995. Locating genes associated with root morphology and drought avoidance in rice via linkage to molecular markers. Theor. Appl. Genet. 90: 969–981.
Choi, D.-W., Zhu, B. and Close, T.J. 1999. The barley (Hordeum vulgare L.) dehydrin multigene family: sequence, allele types, chromosome assignments, and expression characteristics of 11 Dhn genes cv. Dicktoo. Theor. Appl. Genet. 98: 1234–1247.
Choi, H.I., Hong, J.H., Ha, J.O., Kang, J.Y. and Kim, S.Y. 2000. ABFs, a family of ABA-responsive elements binding factors. J. Biol. Chem. 275: 1723–1730.
Close, T.J. 1996. Dehydrins: emergence of a biochemical role of a family of plant dehydration proteins. Physiol. Plant. 97: 795–803.
Cohen, A. and Bray, E.A. 1992. Nucleotide sequence of an ABA-induced tomato gene that is expressed in wilted vegetative organs and developing seeds. Plant Mol. Biol. 18: 411–413.
Grover, A., Pareek, A., Singla, S.L. et al., 1998. Engineering crop plants for tolerance against abiotic stresses through gene manipulation. Curr. Sci. 75: 689–696.
Hagenbeek, D., Quatrano, R.S. and Rock, C.D. 2000. Trivalent ions activate abscisic acid-inducible promoters through an ABI1-dependent pathway in rice protoplasts. Plant Physiol. 123: 1553–1560.
Han, F., Ullrich, S.E., Kleinhof, A., Jones, B.L., Hayes, P.M. and Wesenberg, D.M. 1997. Fine structure mapping of the barley chromosome-1 centromere region containing malting-quality QTLs. Theor. Appl. Genet. 95: 903–910.
Hasegawa, P.M., Bressan, R.A., Zhu, J.-K. and Bohnert, H.J. 2000. Plant cellular and molecular responses to high salinity. Annu. Rev. Plant Physiol. Plant Mol. Biol. 51: 463–499.
Horikawa, I., Tanaka, H., Yuasa, Y., Suzuki, M. and Oshimura, M. 1995. Molecular cloning of a novel human cDNA on chromosome 1q21 and its mouse homolog encoding a nuclear protein with DNA-binding ability. Biochem. Biophys. Res. Commun. 208: 999–1007.
Ingram, J. and Bartels, D. 1996. The molecular basis of dehydration tolerance in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 47: 377–403.
Ishitani, M., Xiong, L., Stevenson, B. and Zhu, J.K. 1997. Genetic analysis of osmotic and cold stress signal transduction in Arabidopsis: interactions and convergence of abscisic acid-dependent and abscisic acid-independent pathways. Plant Cell 9: 1935–1949.
Ishitani, M., Xiong, L., Lee, H., Stevenson, B. and Zhu, J.K. 1998. HOS1, a genetic locus involved in cold-responsive gene expression in Arabidopsis. Plant Cell. 10: 1151–1161.
Kang, H.G. and Singh, K.B. 2000. Characterization of salicylic acid-responsive, Arabidopsis Dof domain proteins: over-expression of OBP3 leads to growth defects. Plant J. 21: 329–339.
Kyte, J. and Doolittle, R.F. 1982. A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157: 105–132.
Lee, H., Xiong, L., Ishitani, M., Stevenson, B. and Zhu, J.K. 1999. Cold-regulated gene expression and freezing tolerance in an Arabidopsis thaliana mutant. Plant J. 17: 301–308.
Liang, P. and Pardee, A.B. 1992. Differential display of eukaryotic messenger RNA by means of polymerase chain reaction. Science 257: 967–971.
Lilley, J.M., Ludlow, M.M., McCouch, S.R. and O'Toole, J.C. 1996. Locating QTLs for osmotic adjustment and dehydration tolerance in rice. J. Exp. Bot. 47: 1427–1436.
Lin, X., Kaul, S., Rounsley, S., Shea, T.P., Benito, M.-I., Town, C.D., Fujii, C.Y., Manson, T., Bowman, C.L., Barnstead, M. et al. 1999. Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana. Nature 402: 761–768.
Liu, Q., Kasuga, M., Sakuma, Y., Abe, H., Miura, S., Yamaguchi-Shinozaki, K. and Shinozaki, K. 1998. Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 binding domain separate two cellular signal transduction pathways in drought and low-temperature-responsive gene expression, respectively, in Arabidopsis. Plant Cell 10: 1391–1406.
Lohrmann, J., Buchholz, G., Keitel, C., Sweere, U., Kircher, S., Baurle, I., Kudla, J., Schafer, E. and Harter, K. 1999. Differential expression and nuclear localization of response regulator-like proteins from Arabidopsis thaliana. Plant Biol. 1: 495–505.
Magaraggia, F., Solinas, G., Valle, G., Giovinazzo, G. and Coraggio, I. 1997. Maturation and translation machanism involved in the expression of a myb gene of rice. Plant Mol. Biol. 35: 1003–1008.
Moore, G. 1995 Cereal genome evolution: pastoral pursuits with 'Lego' genomes. Curr. Opin. Genet. Dev. 5: 717–724.
Morgan, J.M. 1991. A gene controlling differences in osmoregulation in wheat. Aust. J. Plant Physiol. 18: 249–257.
Morgan, J.M. and Tan, M.K. 1996. Chromosomal location of a wheat osmoregulation gene using RFLP analysis. Aust. J. Plant Physiol. 23: 803–806.
Mundy, J. and Chua, N.-H. 1988. Abscisic acid and water-stress induce the expression of a novel rice gene. EMBO J. 7: 2279–2286.
Pan, A., Hayes P.M., Chen F, Chen, T.H.H., Blake, T., Wright, S., Karasai, L. and Bedo, Z. 1994. Genetic analysis of the component of wintherhardiness in barley (Hordeum vulgare L.). Theor. Appl. Genet. 89: 900–910.
Quandt, K., Frech, K., Karas, H., Wingender, E. and Werner, T. 1995. MatInd and MatInspector. New fast and versatile tools for detection of consensus matches in nucleotide sequence data. Nucl. Acids Res. 23: 4878–4884.
Quarrie, S.A., Gulli, M, Calestani, C., Steed, A. and Marmiroli, N. 1994a. Locating of a gene regulating drought-induced abscisic acid production on the long arm of chromosome 5A of wheat. Theor. Appl. Genet. 89: 794–800.
Quarrie, S.A., Lebreton, C., Gulli, M., Calestani, C. and Marmiroli, N. 1994b. QTL analysis of ABA production in wheat and maize and associated physiological traits. Russ. J. Plant Physiol. 41: 565–571.
Rozen, S. and Skaletsky, H. 2000. Primer3 on the WWW for general users and for biologist programmers. Meth. Mol. Biol. 132: 365–386.
Sambrook, J., Fritsch, E.F. and Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Plainview, NY.
Shen, Q. and Ho, T.-H.D. 1995. Functional dissection of an abscisic acid (ABA)-inducible gene reveals two independent ABA-responsive clomplexes each containing a G-box and a novel cis-acting element. Plant Cell. 7: 295–307.
Shen, Q., Zhang, P. and Ho, T.-H.D. 1996. Modular nature of abscisic acid (ABA)-response complex: composite promoter units that are necessary and sufficient for ABA induction of gene expression in barley. Plant Cell 8: 1107–1119.
Shinozaki, K. and Yamaguchi-Shinozaki, K. 2000. Molecular response to dehydration and low temperature: differences and cross-talk between two stress signaling pathways. Curr. Opin. Plant Biol. 3: 217–223.
Straub, P.F., Shen Q. and Ho T.-H.D. 1994. Structure and promoter analysis of an ABA-and stress regulated barley gene, HVA1. Plant Mol. Biol. 26: 617–630.
Teulat, B., This, D., Khairallah, M., Borries, C., Ragot, C., Sourdille, P., Leroy, P., Monneveux, P. and Charrie, A. 1998. Several QTLs involved in osmotic-adjustment trait variation in barley (Horeum vulgare L.). Theor. Appl. Genet. 96: 688–698.
Thomashow, M.F. 1999. Plant cold acclimation: freezing tolerance genes and regulatory mechanisms. Annu. Rev. Plant Physiol. Plant Mol. Biol. 50: 571–599.
Uno, Y., Furihata, T., Abe, H., Yoshida, R„ Shinozaki, K., Yamaguchi-Shinozaki, K. 2000. Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependent signal transduction pathway under drought and high-salinity conditions. Proc. Natl. Acad. Sci. USA 97: 11632–11637.
Urao, T., Yamaguchi-Shinozaki, K., Mitsukawa, N., Shibata, D. and Shinozaki, K. 1996. Molecular cloning and characterization of a gene encodes a MYC-related protein in Arabidopsis. Plant Mol. Biol. 32: 571–576.
Verwoerd, T.C., Dekker, B.M.M., Hoekema, A. 1989. A small-scale procedure for the rapid isolation of plant RNAs. Nucl. Acids Res. 17: 2362.
Vilardell, J., Goday, A., Freire, M.A., Torrent, M., Martinez, M.C., Tornè, J.M. and Pagès, M. 1990. Gene sequence, developmental expression, and protein phosphorylation of RAB-17 in maize. Plant Mol. Biol. 14: 423–432.
Warren, G., McKown, R., Martin, A.L., Teutonico, R. 1996. Isolation of mutations affecting the development of freezing tolerance in Arabidopsis thaliana (L.) Heynh. Plant Physiol. 111: 1011–1019.
Woo, S.S., Jiang, J., Gill, B.S., Paterson, A.H. and Win, R.A. 1994. Construction and characterization of a bacterial artificial chromosome library in Sorghum bicolor. Nucl. Acids Res. 22: 4922–4931.
Xiong, L., Ishitani, M., Lee, H. and Zhu, J.K. 1999. HOS5-a negative regulator of osmotic stress-induced expression in Arabidopsis thaliana. Plant J. 19: 569–578.
Yanagisawa, S. 2000. Dof1 and Dof2 transcription factors are associated with expression of multiple genes involved in carbon metabolism in maize. Plant J. 21: 281–288.
Yanagisawa, S. and Sheen, J. 1998. Involvement of maize Dof zinc finger proteins in tissue-specific and light-regulated gene expression. Plant Cell 10: 75–89.
Yu, Y., Tomkins, J.P., Waugh, R., Frisch, D.A., Kudrna, D., Kleinhofs, A., Brueggeman, R.S., Muehlbauer, G.J., Wise, R.P. and Wing, R.A. 2000. A bacterial artificial chromosome library for barley (Hordeum vulgare L.) and the identification of clones containing putative resistance genes. Theor. Appl. Genet. 101: 1093–1099.
Zhu, J.-K., Liu, J. and Xiong, L. 1998. Genetic analysis of salt tolerance in Arabidopsis: evidence for a critical role of potassium nutrition. Plant Cell 10: 1181–1191.
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Malatrasi, M., Close, T.J. & Marmiroli, N. Identification and mapping of a putative stress response regulator gene in barley. Plant Mol Biol 50, 141–150 (2002). https://doi.org/10.1023/A:1016051332488
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DOI: https://doi.org/10.1023/A:1016051332488